Visit our Expo - Redox and Inflammation signaling 2012

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Session X : Cell death in cancer Poster X, 25 The secret life of caspase-14 G. Denecker, E. Hoste, T. Hochepied, B. Gilbert, P. Ovaere, K. D’Herde1, C. VandenBroucke, S. Lippens, L. Schoonjans2, C. Libert, P. Vandenabeele and W. Declercq Molecular Signalling and Cell Death Unit, Department for Molecular Biomedical Research, Technologiepark 927, B-9052 Ghent-Zwijnaarde (http://www.dmbr.ugent.be), VIB, Ghent University. E-mail: wim.declercq@dmbr.Ugent.be 1 Department of Anatomy, Embryology, Histology, Medical Physics, Ugent. 2 Department of Transgene Technology, KUL, Leuven, Belgium. Caspase-14 is expressed in the differentiating keratinocytes of the epidermis and the hairfollicles. In addition, caspase-14 could also be detected in the Hassall's body's of the thymus. In the skin caspase-14 activation occurs in the uppermost layers of the epidermis and correlates with epidermal cornification. To unravel the possible role of caspase-14 during skin differentiation, we have generated caspase-14-deficient mice. Caspase-14 knock-out mice were born normally with the expected Mendelian ratio. The absence of caspase-14 protein was confirmed both by western blot and immunohistochemistry. Histological sections of the back skin and thymus of newborn wild-type and caspase-14-deficient mice revealed no macroscopical differences. Immunohistochemical analysis of other early and late differentiation markers demonstrated a normal expression pattern of K1, K10, K14, loricrin, involucrin and filaggrin. However, the skin of caspase-14-deficient mice had a more shiny appearance and showed a lichiniform phenotype. Electron microscopic analysis indicated the presence of high numbers composite keratohyalin granules in the caspase-14-deficient epidermis. These granules are normally used as profilaggrin stores. In accordance, caspase-14 mice have an altered profilaggrin-processing pattern. These data argue for a role of caspase- 14 in the final steps of keratinocyte differentiation. Further analysis of different physiological parameters is ongoing in order to unravel the secret life of caspase-14. - 358 -
Session X : Cell death in cancer Poster X, 26 Involvement of PI-3 Kinase signaling in polyunsaturated fatty acid-induced promotion of apoptosis in CaCo-2 cells. Joe-Lin du Toit, Louise Louw and Anna-Mart Engelbrecht Department of Physiological Sciences, University of Stellenbosch, Stellenbosch, 7600, South Africa. E-mail: jl@sun.ac.za The phosphoinositide 3-kinase (PI3-kinase) signaling pathway plays a pivotal role in the regulation of cell growth. A downstream component of the PI3-kinase pathway, PKB/Akt, phosphorylates and regulates the function of various substrates involved in metabolism, proliferation and apoptosis. Evidence has shown that PKB/Akt signaling also contributes to tumourigenesis and cancer progression and that PI3-kinase signaling is up regulated in colon cancer cells. This study aims to elucidate the mechanisms by which various fatty acids influence the PI3-kinase pathway in order to promote apoptosis of colon adenocarcinoma cells and slow down cancer progression. NCM 460 and CaCo-2 cells were cultured and treated with arachidonic acid (AA), oleic acid (OA), palmitic acid (PMA) and docosahexanoic acid (DHA) respectively, and incubated for 48 hours. Samples were analysed by Western blotting with antibodies directed against total PBK/Akt, phospho-PKB/Akt (Ser473 and Thr308), PI3-kinase and various substrates of PKB/Akt as well as their phosphorylated counterparts. Wortmannin (100 nM) was used to inhibit PI3-kinase. Cell viability was assessed with MTT assays. DHA was most effective to significantly increase NCM 460 cell viability whilst decreasing CaCo2 cell viability, followed by AA. With fatty acid treatment, PI3-kinase signaling was up regulated in NCM460 cells and down regulated in CaCo-2 cells, and for both cell lines this was most profound in cells treated with DHA. Phosphorylation of the substrates of PKB/Akt involved in apoptosis (Bad, FKHR) as well as other substrates such as GSK-3# and mTOR were altered in CaCo-2 cells treated with DHA. We propose a potential role for DHA as a therapeutic agent in the treatment of colon cancer. - 359 -
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- 1 - Luxembourg, January 25th to 2
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Table of content PREFACE ..........
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Preface In 1998, we organized the f
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Acknowledgments This meeting has be
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Group B (15h00 - 17h00) Session V.
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- 11 - Exhibition
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Visit our Expo (in alphabetical ord
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Thursday January 26th, 2006 (Early
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Thursday January 26th, 2006 (Evenin
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Regulation of inflammation and gluc
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Saturday January 28th, 2006 (Early
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Oral presentations (by alphabetical
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4: : Lottin S, Vercoutter-Edouart A
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the B-Raf/mitogen-activated/extrace
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Albertini MC, Accorsi A, Citterio B
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AP-1-dependent gene expression in s
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Jaks and cytokine receptors - an in
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The Role of Met-Gab1 Signalling in
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The mammalian tumor suppressor Scri
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SIGNAL TRANSDUCTION BY STRESS-ACTIV
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Title to be announced Caroline Dive
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Mechanisms of DNA methylation in ma
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Blocking the "4 Integrin-Paxillin I
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Regulation of NF-kB-dependent trans
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The immunomodulator AS101 induces g
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Signaling pathways leading to the a
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Boute, N., Jockers, R. and Issad, T
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Jespsen JS, Sorensen MD and Wengel
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8. Nishikawa, H., Kawai, K., Tanaka
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9) Proteome analysis of rat pancrea
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MicroRNA is an anti-apoptotic facto
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Resveratrol inhibits phorbol ester-
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Cross-talk between angiotensin II a
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Multiple role of histamine H 1 rece
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PI3K Targeting by the Beta-GBP Cyto
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D.P. Chopra, R.E. Menard, J. Janusz
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[2] Meijer, L., Flajolet, M. and Gr
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[4] Niemand, C., Nimmesgern, A., Ha
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Oncogenic signaling by mutant p53 M
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activity of the Peroxisome Prolifer
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Restauration of SHIP-1 activity in
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Hypoxia Signaling. Impact on Tumor
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5. Sokolov EI, Zykov KA, Pukhalsky
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HOW ANTITOXIC AND ANTICANCER AGENTS
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Listeria monocytogenes induced Rac1
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Epigenetic Regulation of Stem Cell
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Survey on in vitro cell death induc
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Distinct roles of IRF-3 and IRF-7 i
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Redox-Sensitive Transcription Facto
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Epigenic control of MHC2TA transcri
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Attenuation of MSK1-driven NF-kB ge
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Talking to chromatin: Silencers Sil
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Ubiquitin ligase Smurf1 controls os
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Workshop presentations (by alphabet
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Ambion MicroRNAs (miRNAs) are small
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Organizer: BIOBASE GmbH Date: Janua
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Reliable and efficient gene Knock-d
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Exploring ubiquitin signaling with
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Poster presentations Poster are cla
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Session I : protein domains Poster
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Session II. Receptor signaling and
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Session II : Receptor signaling and
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Session III. Protein kinase cascade
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Session III : Protein kinase cascad
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Session IV. Protein kinase inhibito
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IV. Protein kinase inhibitors: insi
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IV. Protein kinase inhibitors: insi
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V. Phosphatases as key cell signali
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VI. Proteasome degradation pathways
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VI. Proteasome degradation pathways
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Session VII. Stem cell specific cel
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VIII. Inflammation specific signali
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Session XI : Cell death and cardiov
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Session XI: Cell death and cardiova
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Session XII : Cell death and neurod
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Session XIII : Cell signaling pathw
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Session XIV : Transcriptional and t
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Session XV : Reactive oxygen specie
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Session XVI : Chemopreventive agent
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Session XVII : Cell signaling in he
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Dr. Nassera Aouali L-1526 Luxembour
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Dr. Giordano Bianchi Clinic of inte
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Mrs. Isabel Burghardt Laboratory of
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Pr. Czeslaw Cierniewski 92-215 Lodz
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Mr. Igotz Delgado Biochemistry 4894
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Dr. Mark Ginsberg Mail code 0726 92
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Dr. Jochen Hefl Division of Signal
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Mr. Jan Jepsen 2100 Copenhagen DNK
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Pr. Young Min Kim Division of Biolo
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Mr. Christoph Lahtz AG Tumorgenetik
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Pr. Etta Livneh 84105 Beer Sheva IS
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Mrs. Rasa Merzvinskyte Department o
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Mr. Gustav Nilsonne Department of L
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Mrs. Christel Péqueux Tumour and D
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Dr. Gabriella Regis Tumor Immunolog
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Dr. Carsten Scheller 97078 Wuerzbur
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