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Session XI: Cell death and cardiovascular diseases Poster XI, 8
Effects of selenium deficiency on the protein expression of tissues of the cardiovascular
system
A. Kyriakopoulos., A. Richter., C. Wolf., A. Plotnikov., I. Grbavac., D. Behne
Hahn-Meitner-Institut, Dept. “Molecular Trace Element Research in the Life Sciences”
Glienicker Str. 100, D-14109 Berlin, Germany.
Organs of the cardiovascular system such as Heart and aorta contain a lot of mitochondria
(powerhouse of the cell) in which the reactive oxygen species (ROS) are generated. ROS are
damaging the cell. Antioxidantive system protects the cell against the aggressive ROS and
inflammation. Some components of the antioxidative defence system are also metalloenzymes
and selenoenzymes. In order to examine the expression of the heart and aorta proteins (part of
them) in the homogenate of selenium deficient (Se-) and selenium control rats (Se+), 2 D
electrophoresis was used by means of giga gels (30x35 cm). After the electrophoresis the
protein-expression pattern of the (Se-)-gel and (Se+)-gel were compared. The evaluation of
the protein difference was implemented by means of a computer program suitable for the
analysis of protein separated by the two-dimensional electrophoresis. In this way more than
2000 proteins a gel (heart) were detected and more than 1900 protein spots were detected in
the aorta fraction. Ten significant differences were found between the gel of (Se+) and (Se-)-
heart of the rat and more than 15 significant differences between the gel of (Se+) and (Se-) of
the aorta. By means of MALDI-MS/ESI-MS some of these proteins with the different
expression level were determined until now. Of those three proteins were detected as the
alpha myosin heavy chain (alpha-MHC), myosin light chain 1 and 2 (MLC 1 and 2) and the
mitochondrial enzyme creatinin Kinase. First results suggest that selenium deficiency effects
myocardial energy metabolism and contractile proteins.
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