Visit our Expo - Redox and Inflammation signaling 2012

Session IX. Novel compounds targeting inflammatory signaling pathways Poster IX, 4
The Cyclooxygenase-2 Selective Inhibitor Celecoxib Suppresses matrix
Metalloproteinase and Inhibits in Vitro Invasion of the Human Oral Squamous
Carcinoma cells
Young E. Kwak, Nam K. Jeon, Jin Kim and Eun J. Lee
Department of Oral Pathology, Oral Cancer Research Institute, BK21 project for
Medical Science, Yonsei University College of Dentistry, Seoul, KOREA, 120-752
E-mail: e16lee@yumc.yonsei.ac.kr
Cyclooxygenease-2(COX-2) expression is a critical factor in inflammation, and plays an
important role in defense against exogenous stimuli, while overexpression of COX-2 causes
cells to exhibit changes in tumor phenotype. Recently, celecoxib was shown to reduce the risk
of development of cancer and mortality from it. Tumor metastasis is the most important cause
of cancer death. The present study attempted to determine the effects of celecoxib with regard
to human oral squamous cell carcinoma(OSCC) cell growth and invasion/migration. The YD-
10B cells represented a highly-invasive human oral squamous cell carcinoma(OSCC) cell line
which was found to express the COX-2 protein. Celecoxib inhibited cell invasion/migration
through the type 1 collagen matrix by ~ 60% within 24 hours. Gelatin-based zymography
assay reveal that, in the presence of 10uM celecoxib, both MMP-2 and MMP-9 enzyme
activity decreased by ~25-30%. The current in vitro study indicated that the inhibition of
invasion/migration in OSCC cell lines by the cyclooxgenase-2 specific inhibitor, celecoxib,
results in anticancerous effects via a MMP-2 and MMP-9 suppression. In addition, YD-10B
cells could be useful to study the pathological mechanism of OSCC. (This study was
supported by the BK21 project for Medical Science, and by a grant of the national Cancer
control R&D Program 2003(No. 0320230), Ministry of Health & Welfare, Republic of
Korea.)
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