Visit our Expo - Redox and Inflammation signaling 2012

VIII. Inflammation specific signaling Poster VIII, 19
Lack of association of the cyclooxygenase-2 and inducible nitric oxide synthase gene
polymorphism with risk of cervical cancer in Korean population.
Taek Sang Lee a,b, Jae Weon Kima,b, Noh Hyun Parka,b, Soon Beom Kanga,b, Hyo
Pyo Leea,b, Yong Sang Songa,b*
aDepartment of Obstetrics and Gynecology, bCancer Research Institute, College of
Medicine, Seoul National University, Seoul, 110-744, Korea
E-mail: yssong@snu.ac.kr
In recent years, plenty of studies have shown that nitric oxide (NO) and prostaglandin (PG) as
the main inflammatory mediators are involved in various pathophysiological processes
including inflammation and carcinogenesis. In this study, we have explored the impact of
polymorphisms of two representative inflammatory mediators on the risk of cervical cancer.
This study includes 176 cases of histologically confirmed invasive cervical cancer and 172
healthy controls. Allele frequency of twelve SNPs of COX-2 and 5 of iNOS gene in 43
normal populations were analyzed. 14 of 17 shows monomorphic or minimal minor allele
frequency, and therefore we did not perform further analysis for them. Three SNPs ( 2 for
COX-2, 1 for iNOS) were thus chosen for the study. Genotyping of three SNPs (SNP-rs5275
in the untranslated region of exon 10 and rs5277 in the coding region of exon 3 of COX-2,
and iNOS Ser608Leu allele C/T polymorphism within exon 16 of the iNOS reductase
domain) was performed. No significant increase was found in any genotype of the COX-2 and
iNOS in cancer group. We analyzed the correlation between the genotypes and the
clinicopathologic parameters of cervical cancer. No significant association of genotype was
found with respect to each phenotype including clinical stage, lymph node (LN) status,
histologic type, or parametrial invasion. In conclusion, our data shows the lack of significant
evidence of association of the COX-2 and iNOS polymorphisms with cervical cancer in
Korean population.
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