Visit our Expo - Redox and Inflammation signaling 2012

Session XI: Cell death and cardiovascular diseases Poster XI, 5
The effect of different angiotensin converting enzyme inhibitors on subcellular toxicity
of paraquat in isolated rat liver mitochondria
Mahmoud Ghazi-Khansari, Mir Jamal Hossieni , Ali Mohammadi- bardbori
Department of Pharmacology, School of Medicine, Tehran University of Medical
Sciences, Tehran, I.R. Iran. Email: ghazikha@sina.tums.ac.ir
Widespread use of the herbicide paraquat in the recent years has necessitated further studies
on the mechanisms of its toxicity and metabolism. The defect in electron transfer chain of
mitochondria by paraquat is linked to free radical formation. In the present study we
compared the abilities of different angiotensin converting enzyme inhibitor, captopril (a thiol
ACEi), enalapril, and lisinopril (two nonthiol ACEi) on mitochondria toxicity due to paraquat.
The rat liver mitochondria were first isolated by centrifuge (at 4°C at speed of 7000g) in a
mixture of 0.25M saccharose solution and 0.05M Triss buffer. Various concentrations of
paraquat (1, 5, 10µM), enalapril (0.05, 0.5, 0.0.25µM), lisinopril (0.05, 0.01, 0.1µM) and
captopril (0.5, 0.08, 0.1µM) on the mitochondria isolated from the liver with respect to time
were investigated. Paraquat at concentration of 5µM was determined to be significantly
different compared to control (p