Cancer Immune Therapy Edited by G. Stuhler and P. Walden ...

6.4. Phenotypic and Functional Characteristics of Immune Cells Present at the Tumor Site
Fig. 6.4 Proliferative responses of tumor-associated NK cells as
compared to NK cells isolated from normal peripheral blood
mononuclear cells. The [ 3 H]thymidine uptake was determined
after a 3-day culture in the presence of IL-2. The results are mean
c.p.m. counts ± SD of cell cultures established with cells of four
patients and four normal donors. The asteriskindicates a significantly
reduced proliferation of TAL-NK cells as compared to that
of normal NK cells ( p < 0.001). Reproduced with permission from
Lai et al. [46]. © AACR.
Fig. 6.5 Expression of cytokine
genes in ovarian tumor-associated
NK cells (TAL-NK) as compared
with normal peripheral blood lymphocyte
NK cells. The data are
means ± SEM of the ratio between a
cytokine mRNA expression and bactin.
Hot RT-PCR assays were performed
and cytokine mRNA expres-
sion relative to that of b-actin was
calculated after measuring levels of
32 P in a Phosphorimager. The asterisks
indicate significant differences
( p < 0.01) between experimental
and control specimens. Reproduced
with permission from Rabinowich et
al. Int. J. Cancer 1996; 68: 276±284.
© Wiley-Liss, Inc.
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