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Cancer du sein et micro-environnement tumoral: rôle de la protéase ...

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LRP1, Adipogenesis, ObesityLipolysis AssayCells were incubated in DMEM without serum in the presenceof 2% fatty acid-free bovine serum albumin (A7030, Sigma)overnight. Fresh medium containing isoproterenol was ad<strong>de</strong>d for90 min. Glycerol and NEFA contents in the medium weremeasured with free glycerol reagent (F6428 Sigma) and NEFA Ckit (Wako Chemicals, Germany).References1. Kahn SE, Hull RL, Utzschnei<strong>de</strong>r KM (2006) Mechanisms linking obesity toinsulin resistance and type 2 diab<strong>et</strong>es. Nature 444(7121): 840–846.2. He W, Barak Y, Hevener A, Olson P, Liao D, <strong>et</strong> al. (2003) Adipose-specificperoxisome proliferator-activated receptor gamma knockout causes insulinresistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712–15717.3. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM (1994) mPPARgamma 2: tissue-specific regu<strong>la</strong>tor of an adipocyte enhancer. Genes Dev 8(10):1224–1234.4. 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