Cancer du sein et micro-environnement tumoral: rôle de la protéase ...

More documents

Recommendations

Info

ABcath-D mRNA(ratio RS9)PPARg mRNA(ratio RS9)aP2 mRNA(ratio RS9)864202520151050100806040200cath-DPPARgaP2Ex Co D1 D2 D3 D7 D10 D14 D17days relative to confluenceEx Co D1 D 2 D3 D7 D10 D14 D17** **cellular cath-Dsecreted cath-DHSLb actinERK2C8h 24h 48hInsulin - + - + - +cath-DERK2Figure 3
Figure 3. Cath-D expression increases during adipogenesis of NIH-3T3F442Apreadipocytes(A)Cath-D mRNA expression during adipogenesis. RNA expression of cath-D,PPARg and aP2 were analysed in exponentially growing NIH-3T3F442Apreadipocytes (Ex), in NIH-3T3F442A grown to confluence (Co) and after theindicated time of culture in adipogenic differentiation medium by real-timequantitative RT-PCR. Mean ± SD of 4 independent experiments is shown. P
Page 1 and 2:
Université Montpellier I UFR Méd
Page 3 and 4:
Un grand merci à tous mes collabor
Page 5 and 6:
TitleBreast cancer and tumoral micr
Page 7 and 8:
Cancer du sein et micro-environneme
Page 9 and 10:
C. PRESENTATION DU TRAVAIL DE THESE
Page 11 and 12:
But de la thèseLa cathepsine-D (ca
Page 13 and 14:
I. Le tissu adipeux1) Généralité
Page 15 and 16:
(Adenosine triphosphate). Cette pro
Page 18 and 19:
Cette synthèse de novo a lieu dans
Page 20 and 21:
ABFigure 6 : Schéma de la oxydati
Page 22 and 23:
d. Ladipocyte : une cellule sécré
Page 24 and 25:
3) Ladipogenèsea. Les différentes
Page 26 and 27:
. Les facteurs de transcriptionLa d
Page 28 and 29:
Lexpression de C/EBP est quant à e
Page 30 and 31:
adipeux. Les analyses histologiques
Page 32 and 33:
adipocyteFigure 10 : Schéma repré
Page 34 and 35:
II. La cathepsine-D1) Synthèse, ma
Page 36 and 37: les cath-B et L, en une forme matur
Page 38 and 39: Il existe deux RM6P : le RM6P/IGFII
Page 40 and 41: 2) Fonctions de la cath-Da. Dans la
Page 42 and 43: . Dans les pathologiesEn plus de se
Page 44 and 45: c. Rôles et mécanismes daction da
Page 46 and 47: carcinome de prostate serait respon
Page 48 and 49: La cath-D joue donc un rôle import
Page 50 and 51: Une fois le marquage des peptides e
Page 52 and 53: Afin de réaliser ce projet, nous a
Page 54 and 55: III. Le récepteur LRP11) Organisat
Page 56 and 57: 2) Trafic intra-cellulaireComme le
Page 58 and 59: LRP1αLRP1Membrane associatedprotea
Page 60 and 61: Tableau 3 : Ligands connus du LRP1(
Page 62 and 63: I. Etude du rôle de la cathepsine
Page 64 and 65: Cathepsin-D, a key protease in brea
Page 66 and 67: IntroductionConsumption of meals ri
Page 68 and 69: (Fig. 1A, panel a). This differenti
Page 70 and 71: differentiated adipocyte (Fig. 4B).
Page 72 and 73: DiscussionOur results demonstrate t
Page 74 and 75: from normal and peri-al breast adip
Page 76 and 77: mouse RS9 (sens 5CGGCCCGGGAGCTGTTGA
Page 78 and 79: agreement of local ethic committee.
Page 80 and 81: Statistical analysis. Results are e
Page 82 and 83: Loncarek J, Freiss G, Vignon F and
Page 84 and 85: Aa3000 *b4000*B8000**cath-D mRNA(ar
Page 88 and 89: A D3 D7 D14D0BaD0 D3 D7 D14D0 D3 D7
Page 90 and 91: AshLucshcath-DF442A C34 C37 A4 D10c
Page 92 and 93: AF442-AC34C37A4D10BF442-AC34C37A4D1
Page 94 and 95: II. Etude du rôle du LRP1 dans les
Page 96 and 97: 2) Article 2:LRP1 receptor controls
Page 98 and 99: LRP1, Adipogenesis, Obesityrelative
Page 100 and 101: LRP1, Adipogenesis, ObesityFigure 3
Page 102 and 103: LRP1, Adipogenesis, ObesityFigure 5
Page 104 and 105: LRP1, Adipogenesis, ObesitySome ins
Page 106 and 107: LRP1, Adipogenesis, ObesityLipolysi
Page 108 and 109: CONCLUSIONLa cathepsine D (cath-D)
Page 110 and 111: la souris obèses. Enfin, linhibiti
Page 112 and 113: carcinogénique sont encore mal con
Page 114 and 115: Ce travail de thèse a étudié, po
Page 116 and 117: REFERENCESAhima, R. S. (2006). Adip
Page 118 and 119: implicates them as antigen presenti
Page 120 and 121: Cataldo, A. M., Barnett, J. L., Ber
Page 122 and 123: Folkman, J. (2003). Fundamental con
Page 124 and 125: Hofmann, S. M., Zhou, L., Perez-Til
Page 126 and 127: Langin, D. (2006a). Adipose tissue
Page 128 and 129: Ludwig, T., Ovitt, C. E., Bauer, U.
Page 130 and 131: Nirde, P., Derocq, D., Maynadier, M
Page 132 and 133: Rozanov, D. V., Hahn-Dantona, E., S
Page 134 and 135: Taleb, S., Cancello, R., Clement, K
Page 136 and 137:
Xiao, Y., Junfeng, H., Tianhong, L.
Page 138 and 139:
F. ANNEXE98
Page 140 and 141:
Cathepsin D is a new ligand for ext
Page 142 and 143:
INTRODUCTIONLysosomal aspartic prot
Page 144 and 145:
pcDNA3.1(+)Myc-tagged LRP1b into pc
Page 146 and 147:
(Protein refolding kit, Novagen) fo
Page 148 and 149:
anti-mouse-gold (Aurion). Sections
Page 150 and 151:
not secrete detectable levels of pr
Page 152 and 153:
using cath-D-/- MEF transfected wit
Page 154 and 155:
co-culture outgrowth assays with ca
Page 156 and 157:
REFERENCES1. Vignon, F., Capony, F.
Page 158 and 159:
steps in vivo: proliferation, angio
Page 160 and 161:
28. Laurent-Matha, V., Lucas, A., H
Page 162 and 163:
42. Zurhove, K., Nakajima, C., Herz
Page 164 and 165:
Figure 1. Cath-D interacts with the
Page 166 and 167:
Figure 2. Cath-D binds to residues
Page 168 and 169:
Figure 3. Cath-D interacts with LRP
Page 170 and 171:
Figure 5. Silencing LRP1 in cath-D
Page 172 and 173:
Figure 1. Cath-D interacts with the
Page 174 and 175:
Figure 6. LRP1 is the receptor medi
Page 176 and 177:
Beaujouin, Figure Sup. 2cath-D-/-ME
Page 178:
RésuméLaspartyl protéase catheps
show all

Cancer du sein et micro-environnement tumoral: rôle de la protéase ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?