Cancer du sein et micro-environnement tumoral: rôle de la protéase ...

More documents

Recommendations

Info

28. Laurent-Matha, V., Lucas, A., Huttler, S., Sandhoff, K., Garcia, M., andRochefort, H. Procathepsin D interacts with prosaposin in cancer cells but itsinternalization is not mediated by LDL receptor-related protein. Exp Cell Res,277: 210-219, 2002.29. Herz, J. The switch on the RAPper's necklace. Mol Cell, 23: 451-455, 2006.30. Dedieu, S., Langlois, B., Devy, J., Sid, B., Henriet, P., Sartelet, H., Bellon, G.,Emonard, H., and Martiny, L. LRP-1 silencing prevents malignant cell invasiondespite increased pericellular proteolytic activities. Mol Cell Biol, 28: 2980-2995, 2008.31. Song, H., Li, Y., Lee, J., Schwartz, A. L., and Bu, G. Low-density lipoproteinreceptor-related protein 1 promotes cancer cell migration and invasion byinducing the expression of matrix metalloproteinases 2 and 9. Cancer Res, 69:879-886, 2009.32. Benes, P., Jurajda, M., Zaloudik, J., Izakovicova-Holla, L., and Vacha, J.C766T low-density lipoprotein receptor-related protein 1 (LRP1) genepolymorphism and susceptibility to breast cancer. Breast Cancer Res, 5: R77-81, 2003.33. Montel, V., Gaultier, A., Lester, R. D., Campana, W. M., and Gonias, S. L. Thelow-density lipoprotein receptor-related protein regulates cancer cell survivaland metastasis development. Cancer Res, 67: 9817-9824, 2007.34. Koong, A. C., Denko, N. C., Hudson, K. M., Schindler, C., Swiersz, L., Koch,C., Evans, S., Ibrahim, H., Le, Q. T., Terris, D. J., and Giaccia, A. J. Candidategenes for the hypoxic tumor phenotype. Cancer Res, 60: 883-887, 2000.21
35. Bando, H., Toi, M., Kitada, K., and Koike, M. Genes commonly upregulated byhypoxia in human breast cancer cells MCF-7 and MDA-MB-231. BiomedPharmacother, 57: 333-340, 2003.36. Hu, K., Yang, J., Tanaka, S., Gonias, S. L., Mars, W. M., and Liu, Y. Tissuetypeplasminogen activator acts as a cytokine that triggers intracellular signaltransduction and induces matrix metalloproteinase-9 gene expression. J BiolChem, 281: 2120-2127, 2006.37. Boucher, P. and Gotthardt, M. LRP and PDGF signaling: a pathway toatherosclerosis. Trends Cardiovasc Med, 14: 55-60, 2004.38. Boucher, P., Liu, P., Gotthardt, M., Hiesberger, T., Anderson, R. G., and Herz,J. Platelet-derived growth factor mediates tyrosine phosphorylation of thecytoplasmic domain of the low Density lipoprotein receptor-related protein incaveolae. J Biol Chem, 277: 15507-15513, 2002.39. Yang, M., Huang, H., Li, J., Li, D., and Wang, H. Tyrosine phosphorylation ofthe LDL receptor-related protein (LRP) and activation of the ERK pathway arerequired for connective tissue growth factor to potentiate myofibroblastdifferentiation. Faseb J, 18: 1920-1921, 2004.40. Barnes, H., Ackermann, E. J., and van der Geer, P. v-Src induces Shc bindingto tyrosine 63 in the cytoplasmic domain of the LDL receptor-related protein 1.Oncogene, 22: 3589-3597, 2003.41. May, P., Reddy, Y. K., and Herz, J. Proteolytic processing of low densitylipoprotein receptor-related protein mediates regulated release of itsintracellular domain. J Biol Chem, 277: 18736-18743, 2002.22
Page 1 and 2:
Université Montpellier I UFR Méd
Page 3 and 4:
Un grand merci à tous mes collabor
Page 5 and 6:
TitleBreast cancer and tumoral micr
Page 7 and 8:
Cancer du sein et micro-environneme
Page 9 and 10:
C. PRESENTATION DU TRAVAIL DE THESE
Page 11 and 12:
But de la thèseLa cathepsine-D (ca
Page 13 and 14:
I. Le tissu adipeux1) Généralité
Page 15 and 16:
(Adenosine triphosphate). Cette pro
Page 18 and 19:
Cette synthèse de novo a lieu dans
Page 20 and 21:
ABFigure 6 : Schéma de la oxydati
Page 22 and 23:
d. Ladipocyte : une cellule sécré
Page 24 and 25:
3) Ladipogenèsea. Les différentes
Page 26 and 27:
. Les facteurs de transcriptionLa d
Page 28 and 29:
Lexpression de C/EBP est quant à e
Page 30 and 31:
adipeux. Les analyses histologiques
Page 32 and 33:
adipocyteFigure 10 : Schéma repré
Page 34 and 35:
II. La cathepsine-D1) Synthèse, ma
Page 36 and 37:
les cath-B et L, en une forme matur
Page 38 and 39:
Il existe deux RM6P : le RM6P/IGFII
Page 40 and 41:
2) Fonctions de la cath-Da. Dans la
Page 42 and 43:
. Dans les pathologiesEn plus de se
Page 44 and 45:
c. Rôles et mécanismes daction da
Page 46 and 47:
carcinome de prostate serait respon
Page 48 and 49:
La cath-D joue donc un rôle import
Page 50 and 51:
Une fois le marquage des peptides e
Page 52 and 53:
Afin de réaliser ce projet, nous a
Page 54 and 55:
III. Le récepteur LRP11) Organisat
Page 56 and 57:
2) Trafic intra-cellulaireComme le
Page 58 and 59:
LRP1αLRP1Membrane associatedprotea
Page 60 and 61:
Tableau 3 : Ligands connus du LRP1(
Page 62 and 63:
I. Etude du rôle de la cathepsine
Page 64 and 65:
Cathepsin-D, a key protease in brea
Page 66 and 67:
IntroductionConsumption of meals ri
Page 68 and 69:
(Fig. 1A, panel a). This differenti
Page 70 and 71:
differentiated adipocyte (Fig. 4B).
Page 72 and 73:
DiscussionOur results demonstrate t
Page 74 and 75:
from normal and peri-al breast adip
Page 76 and 77:
mouse RS9 (sens 5CGGCCCGGGAGCTGTTGA
Page 78 and 79:
agreement of local ethic committee.
Page 80 and 81:
Statistical analysis. Results are e
Page 82 and 83:
Loncarek J, Freiss G, Vignon F and
Page 84 and 85:
Aa3000 *b4000*B8000**cath-D mRNA(ar
Page 86 and 87:
ABcath-D mRNA(ratio RS9)PPARg mRNA(
Page 88 and 89:
A D3 D7 D14D0BaD0 D3 D7 D14D0 D3 D7
Page 90 and 91:
AshLucshcath-DF442A C34 C37 A4 D10c
Page 92 and 93:
AF442-AC34C37A4D10BF442-AC34C37A4D1
Page 94 and 95:
II. Etude du rôle du LRP1 dans les
Page 96 and 97:
2) Article 2:LRP1 receptor controls
Page 98 and 99:
LRP1, Adipogenesis, Obesityrelative
Page 100 and 101:
LRP1, Adipogenesis, ObesityFigure 3
Page 102 and 103:
LRP1, Adipogenesis, ObesityFigure 5
Page 104 and 105:
LRP1, Adipogenesis, ObesitySome ins
Page 106 and 107:
LRP1, Adipogenesis, ObesityLipolysi
Page 108 and 109:
CONCLUSIONLa cathepsine D (cath-D)
Page 110 and 111: la souris obèses. Enfin, linhibiti
Page 112 and 113: carcinogénique sont encore mal con
Page 114 and 115: Ce travail de thèse a étudié, po
Page 116 and 117: REFERENCESAhima, R. S. (2006). Adip
Page 118 and 119: implicates them as antigen presenti
Page 120 and 121: Cataldo, A. M., Barnett, J. L., Ber
Page 122 and 123: Folkman, J. (2003). Fundamental con
Page 124 and 125: Hofmann, S. M., Zhou, L., Perez-Til
Page 126 and 127: Langin, D. (2006a). Adipose tissue
Page 128 and 129: Ludwig, T., Ovitt, C. E., Bauer, U.
Page 130 and 131: Nirde, P., Derocq, D., Maynadier, M
Page 132 and 133: Rozanov, D. V., Hahn-Dantona, E., S
Page 134 and 135: Taleb, S., Cancello, R., Clement, K
Page 136 and 137: Xiao, Y., Junfeng, H., Tianhong, L.
Page 138 and 139: F. ANNEXE98
Page 140 and 141: Cathepsin D is a new ligand for ext
Page 142 and 143: INTRODUCTIONLysosomal aspartic prot
Page 144 and 145: pcDNA3.1(+)Myc-tagged LRP1b into pc
Page 146 and 147: (Protein refolding kit, Novagen) fo
Page 148 and 149: anti-mouse-gold (Aurion). Sections
Page 150 and 151: not secrete detectable levels of pr
Page 152 and 153: using cath-D-/- MEF transfected wit
Page 154 and 155: co-culture outgrowth assays with ca
Page 156 and 157: REFERENCES1. Vignon, F., Capony, F.
Page 158 and 159: steps in vivo: proliferation, angio
Page 162 and 163: 42. Zurhove, K., Nakajima, C., Herz
Page 164 and 165: Figure 1. Cath-D interacts with the
Page 166 and 167: Figure 2. Cath-D binds to residues
Page 168 and 169: Figure 3. Cath-D interacts with LRP
Page 170 and 171: Figure 5. Silencing LRP1 in cath-D
Page 172 and 173: Figure 1. Cath-D interacts with the
Page 174 and 175: Figure 6. LRP1 is the receptor medi
Page 176 and 177: Beaujouin, Figure Sup. 2cath-D-/-ME
Page 178: RésuméLaspartyl protéase catheps
show all

Cancer du sein et micro-environnement tumoral: rôle de la protéase ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?