Chapter 1, The Reptilian Spectacle - UWSpace - University of ...

kDa
76 -
52 -
38 -
31 -
24 -
17 -
12 -
8.5 -
kDa
76 -
52 -
38 -
31 -
24 -
17 -
12 -
8.5 -
pH
pH
3.5 4 5 6 7 8 9 10
| | | I I I I I
3.5 4 5 6 7 8 9 10
| | | I I I I I
97
A
B
Figure 4-15. 2D
electrophoresis of
coachwhip spectacle
scales. A: 3.75 µg of
loaded protein to
adequately resolve the
main ß keratins. B: 12.5
µg of loaded protein and
image enhancement to
resolve low
concentration proteins
and isoforms. The
majority of ß keratins are
grouped between pH 4-5
and at 12 kDa. Several
12 kDa ß keratin
isoforms are present at
higher pH and may
correspond to different
post-translational
modifications.