Chapter 1, The Reptilian Spectacle - UWSpace - University of ...

kDa
76 -
52 -
38 -
31 -
24 -
17 -
12 -
8.5 -
kDa
76 -
52 -
38 -
31 -
24 -
17 -
12 -
8.5 -
pH
pH
3.5 4 5 6 7 8 9 10
| | | I I I I I
3.5 4 5 6 7 8 9 10
| | | I I I I I
98
A
B
Figure 4-16. 2D
electrophoresis of
coachwhip parietal
scales. A: 3.75 µg of
loaded protein to
adequately resolve the
main ß keratins. B: 12.5
µg of loaded protein and
image enhancement to
resolve low concentration
proteins and isoforms. As
with the spectacle scale,
most ß keratins are
grouped between pH 4-5
and weight in the range
of 12 kDa. Comparatively
fewer ß keratin isoforms
are present at higher pH,
suggesting less (or
different) post-
translational
modifications taking
place.