Chapter 1, The Reptilian Spectacle - UWSpace - University of ...

3.4.5 Physical reasons for variation in λ50% 73
Chapter 4, Biochemical Analysis of the Spectacle Scale with an Emphasis on Beta(ß)
Keratins 79
4.1 Introduction 80
4.2 Methods & Materials 84
4.2.1 Sample Collection and Solubilization 84
4.2.2 SDS-PAGE 85
4.2.3 Electrophoresis 85
4.2.4 Western Blotting 85
4.2.5 2-Dimensional Electrophoresis 86
4.2.6 Isoelectric Focusing (IEF) 86
4.2.7 2nd Dimension (SDS-PAGE) 86
4.3 Results 88
4.3.1 Keratins of Coachwhip and Corn Snake Scales 88
4.3.2 Keratins of Gecko Spectacle, Labial and Head scales. 91
4.3.3 Comparative Assessment of Snake Spectacle ß Keratins 93
4.3.4 2D Electrophoretic Comparison of Coachwhip Snake Spectacle and Parietal Scale
Proteins 96
4.4 Discussion 99
4.4.1 Keratins of the Snake Spectacle Scale Versus Other Scales 99
4.4.2 Comparative Investigation of Spectacle ß Keratins 100
4.4.3 Keratins of Gecko Scales 101
4.4.4 Conclusion 103
Chapter 5, Summary and Concluding Remarks 104
References 108
Appendix A One cycle of spectacle blood flow in the resting coachwhip 128
Appendix B Spectacle blood flow in a juvenile corn snake during the renewal phase
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