1.1 Porphyrins - Friedrich-Alexander-Universität Erlangen-Nürnberg

Experimental Section 6
6 Experimental Section
6.1 Chemicals, Methods and Equipment
Chemicals were purchased from SIGMA-ALDRICH, FLUKA or ACROS ORGANICS and used without
further purification unless otherwise stated. Dichloromethane, chloroform and ethyl acetate
were freshly distilled from K2CO3, methanol from CaCl2 and acetone from MgSO4. Absolute
solvents were dried by common literature procedures and stored under inert gas
atmospheres. Spirulina platensis algae were purchased from Spirulife (www.spirulife.de),
VitaNatura (www.vitanatura.de) or Xanazon (www.xanazon.de) via Internet.
For purification, characterization and further investigations of/on the synthesized
compounds, the following methods and equipment were used.
Thin Layer Chromatography (TLC) was performed on aluminum sheets coated with silica
gel 60 F254 purchased from MERCK. Visualization was performed by using an UV lamp (254 or
366 nm) or by developing (1% KMnO4 in aqueous solution acidified with H2SO4 or elemental
bromine).
Flash Column Chromatography (FC) was done on silica gel 60 (230-400 mesh, 0.04-
0.063 nm) purchased from either ICN or MACHEREY-NAGEL. Eluents were purified in advance as
stated above.
NMR spectroscopy was conducted on machines from JEOL (EX 400, GX 400) or BRUKER
(AVANCE 300, AVANCE 400). The chemical shifts are given in ppm with the used solvents as
references. Multiplicities are denoted “s” (singlet), “d” (doublet), “t” (triplet), “q” (quartet),
“m” (multiplet) or as combinations thereof. Signals annexed “br” are not clearly resolved or
significantly broadened. Peripheral aryl moieties are referred to as “Ar” whereby primes
indicate higher substituted phenyl rings. The raw data was processed by using MESTRE-C or
ACD Labs.
IR spectroscopy was conducted on an ASI React IR 1000 (Analytical Services Inc.).
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