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Organohalogen concentrations and a gross and histologic ...

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No. skulls collected<br />

50<br />

45<br />

40<br />

35<br />

30<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

velopmental instability (i.e. the magnitude of FA) could have been initiated<br />

(e.g. Siegel <strong>and</strong> Doyle 1975a-c, Doyle et al., 1977, Siegel et al., 1977a). The<br />

model was successively reduced for non-significant interactions (p>0.05) <strong>and</strong><br />

the significance was evaluted from the remaining reduced model.<br />

2.5.6 FA versus contaminants<br />

For the 94 samples from 1999 to 2002 of which both skulls <strong>and</strong> contaminant<br />

analysis were available a non-parametric Spearman correlation coefficient<br />

was used to test for significant correlations between levels of individual<br />

organohalogens <strong>and</strong> FA. Due to the large numbers of tests (k=98) a Bonferroni<br />

correction of the p-estimates was applied to avoid the generation of<br />

significant results simply by chance. The critical alpha level was set to<br />

p=0.05/98=0.0005.<br />

3. Results<br />

3.1 The sample<br />

Before analysing the trend of FA between periods (before <strong>and</strong> after 1960)<br />

<strong>and</strong> age/sex groups, individuals were grouped as being adult males (≥6<br />

years), adult females (≥5 years) <strong>and</strong> the remaining individuals of both sexes<br />

as subadults (Table 2). The sample consisted of a total of 283 individuals<br />

distributed as 142 subadults, 78 adult males <strong>and</strong> 63 adult females (Table 2).<br />

The number of skulls <strong>and</strong> age of individuals were equally distributed over<br />

time with few peaks, although the period 2000-2002 was over-represented<br />

(Fig. 2).<br />

92 08 25 28 33 36 60 66 69 73 85 99 02<br />

Year<br />

Table 2<br />

Age, sex <strong>and</strong> number of polar bear skulls divided on periods. The number of subadults <strong>and</strong><br />

females differ in particular between two periods investigated.<br />

Period/group Subadults Females Males Sum<br />

1892-1960 45 13 37 94<br />

1961-2002 97 50 41 189<br />

Sum 142 63 78 283<br />

Figure 2<br />

Number of skulls collected per year from 1892 to 2002 (n=283) (left) <strong>and</strong> their individual age (right).<br />

Age (years)<br />

30<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

1880 1900 1920 1940 1960 1980 2000 2020<br />

Year<br />

101

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