A new approach to species delimitation in Septoria - CBS - KNAW

A new approach to species delimitation in SeptoriaFig. 5. Caryophylloseptoria spergulae. A, B. Colonies CBS 109010. A. On OA. B. On MEA. C. Conidia and conidiogenous cells in planta (CBS H-21150). D–H. Conidia on OA(CBS 109010). Scale bars = 10 µm.granular material and large vacuoles in the living state, oil-dropletsmerged into larger guttules in the rehydrated state, (18–)24–33(–40) × 2.0–2.5(–3.0) µm (rehydrated). Sexual morph unknown.Description in vitro: Colonies on OA less than 2 mm diam after 2wk (6–8 mm in 28 d), restricted, though not much elevated, withan even, colourless, glabrous margin; colony surface covered bya dense continuous or discontinuous mat of grey, finely felted tosomewhat woolly, low aerial mycelium, agar around the colonyshowing a yellow diffusing pigment; immersed mycelium paleluteous to saffron, reverse concolorous, but olivaceous-blackunder areas with well-developed aerial mycelium or conidiomata.Colony sporulating in the centre after about 2 wk, with spores inlarge pale salmon droplets oozing from pycnidioid complexes.Colonies on CMA 8–10 mm diam in 28 d, as on OA, but immersedmycelium soon darkening and olivaceous-black, while the aerialmycelium is somewhat more greenish, and the mat denser andmore continuous; reverse olivaceous-black. Colonies on MEA 5mm diam in 2 wk (8–10 mm in 28 d), restricted, with an even, buff,glabrous margin; colony surface black, but with a diffuse mat ofgreyish white, often with some sulphur yellow (centre), woolly aerialmycelium; fruitbodies developing tardily on the colony surface,sporulating with large, dirty white to pale reddish masses in waterydroplets; reverse dark brick to olivaceous-black. Colonies on CHA7–9 mm diam in 2 wk, as on MEA, but aerial mycelium higher anddenser, in the centre also conspicuously yellowish-pale citrine. Nosporulation observed.Conidiomata mostly olivaceous-brown, irregular mergedcomplexes of initially closed, but soon widely opening stromata,only rarely pycnidial and structurally similar to those on the naturalsubstratum. Conidiogenous cells hyaline, ampuliform, or elongatedampulliform with a relatively long neck, hyaline or very pale brownwww.studiesinmycology.org237