A new approach to species delimitation in Septoria - CBS - KNAW

More documents

Recommendations

Info

Verkley et al.Fig. 10. Septoria astragali, CBS 109116. A–C. Colonies (15 C, nUV). A. On OA. B. On CHA. C. On MEA. D. Conidia and conidiogenous cells on OA (CBS 109116); E–G. Conidiain planta (CBS H-21258). H. Conidiogenous cells on OA (CBS 123878). I. Conidia on OA (CBS 123878). Scale bars = 10 µm.walls covered with short mycelial outgrowth, with a single openingreleasing a stout cirrhus of pale whitish to rosy-buff conidial slime;reverse mostly olivaceous-black due to the conidiomata; afterincubation of 5–7 wk, more of the immersed mycelium darkensto olivaceous-black, with traces of a red pigment especially nearthe margin, and the aerial mycelium becomes more dominant,white or grey. Colonies on CMA 2–3 mm diam in 10 d (27–28 mmin 7 wk), as on OA, but the reddish pigment at the margin moreconspicuous in old cultures. Colonies on MEA 1.5–3 mm diam in 10d ((8–)14–17 mm in 7 wk), with an even to irregular, glabrous, buffmargin; colonies first restricted, while later faster growing hyphalstrands colonize the medium underneath the surface of the agar,pustulate to hemispherical, the surface first ochreous or amber,later olivaceous-grey or black covered by fairly dense, short,white aerial mycelium; some superficial or immersed pycnidialconidiomata formed, releasing cirrhi of pale buff conidial slime;reverse dark umber to brown-vinaceous. Colonies on CHA 1.5–3mm diam in 10 d (15–17 mm in 7 wk), with an irregular marginwhich is hardly visible from above; colonies restricted, irregularlypustulate to hemispherical, the surface dark brick to dark slate244
A new approach to species delimitation in Septoriablue, covered by a diffuse, very short, felty, white aerial mycelium;abundant superficial conidiomata releasing stout cirrhi of rosy-buffconidial slime; reverse blood colour.Hosts: Astragalus spp.Material examined: Austria, Tirol, Ötztal, Ötz, near Habichen W of Ötztaler Aache,1 Aug. 2000, on living leaves of Astragalus glycyphyllos, G. Verkley 1036, epitypedesignated here CBS H-21151 “MBT175673”, living cultures ex-epitype CBS109116, 109117; Carinthia, near Töschling at Wörthersee, on living leaves of A.glycyphyllos, July (year not indicated), Keissler, distributed in Keissler, Kryptogam.exsicc. 1331, PC 0084566. Czech Republic, Moravia, Pavlov, forest around ruin, 18Sep. 2008, on living leaves of A. glycyphyllos, G. Verkley 6023, CBS H-21258, livingculture CBS 123878. France, Lower Normandy, Calvados, Baynes near Forêt deCerisy, 20-21 Sep. 1842, on leaves of A. glycyphyllos, Roberge, “Col. Desmazieres1863, no. 8, 59”, isotype PC 0084563; Côte-d’Or, Montagne de Bard, same substr.,June 1901, Fautrey, PC 0084565 (herb. Mussat); same substr., Pinsguel, nearToulouse, 30 Aug. 1935, Moesz, PC 0084564. Poland, Puszcza Bialowieska, Aug.1922, on living leaves of A. glycyphyllos, W. Siemaszko, distributed in W. Siemaszko,Fung. Bialowiezenses exsicc. 73, PC 0084569. Romania, Transsilvania, distr.Istriţa-Năsăud, Arcalia Arboretum, 1 July 1966, on living leaves of A. glycyphyllos,A. Crişan, distributed in Flora Romania exsicc 3127, PC 0084567; same substr.,Muntenia, distr. Ilfov, Pantelimon, 18 July 1926, T. Săvulescu & C. Sandhu,distributed in Săvulescu, Herb. Mycol. Romanicum 4, 166, PC 0084568 (sub S.astragali f. santonensis).Notes: The type specimen in PC of S. astragali contains severalmounted leaves and is provided with a hand-written description inFrench. Conidia observed in this material are mostly 7–9-septate,85–130 × 2.5–3.5 μm. The type thus agrees well with the originaldescription which indicated conidia 120 × 3 µm, with 9–10 septa.Of the other collections available for this study that generallyall agree with the type in morphology and leaf symptoms, 1036from Tirol is chosen as epitype. Various authors have reportedcomparable conidial measurements for this large-spored Septoria.Jørstad (1965) reported conidial measurements 48–128 × 3–3.5µm, Teterevnikova-Babayan (1987) 60–140 × 3–4 µm, Vanev et al.(1997), 58–112 × 2.5–3.5 µm. According to the original diagnosis,S. astragali var. brencklei, described from Lathyrus venosus inNorth Dakota, has 8–10-septate conidia, 130–150 × 4–5 µm,and Teterevnikova-Babayan (1987) placed it in synonymy with S.astragali. Septoria astragali is one of the first of over 200 Septoriathat were described from plants of the family Fabaceae.Septoria campanulae (Lév.) Sacc., Syll. Fung. 3: 544. 1884.Fig. 11.Basionym: Ascochyta campanulae Lév., Annls Sci. Nat., sér. 3, Bot.5: 277. 1846.Description in planta: Symptoms definite, circular to irregular, paleto dark brown leaf spots, epigenous, usually delimited by blackenedveinlets. Conidiomata pycnidial, predominantly ephiphyllous, rarelyhyphyllous, scattered, globose to subglobose, immersed to semiimmersed,40–125 µm diam; ostiolum circular, central, 10–20 µmwide, surrounding cells darker; conidiomatal wall 10–20 µm diam,composed of an outer layer of brown-walled cells 3.5–10 µm diam,and an inner layer of hyaline cells 3.5–6 µm diam. Conidiogenouscells discrete or integrated in 1–2-septate conidiophores, cylindrical,or ampuliform, sometimes with an elongated neck, hyaline,holoblastic, proliferating sympodially, and often in the same cellalso percurrently showing indistinct annellations, 5–15 × 3–5 µm.Conidia filiform, straight or slightly curved, gradually attenuated toa narrowly rounded or somewhat pointed apex, gradually or moreabruptly attenuated into a narrowly truncate base, 0–1(–3)-septate,not or indistinctly constricted around the septa, hyaline, contentsFig. 11. Septoria campanulae. A. Conidia and conidiogenous cells in planta (CBSH-21178). B. Ibid., on CHA (CBS 109114). Scale bars = 10 µm.with small oil-droplets and minutely granular material in theliving state and rehydrated state, (12.5–)15–25(–32) × 1.5–2 µm(rehydrated). Sexual morph unknown.Description in vitro: Colonies on OA 6–9 mm diam in 10 d (28–32mm in 3 wk; > 65 mm in 7 wk), with an even, somewhat undulating,glabrous, colourless margin; colonies spreading, the surface plane,immersed mycelium pale luteous to ochreous, but radiating greenishor olivaceous hyphal strands soon developing, which later dominatethe olivaceous-black colonies, then also a distinct red pigment isproduced which diffuses beyond the colony margin; scattered, mostlysuperficial pycnidial conidiomata, which are first dark olivaceous, thenwww.studiesinmycology.org245
Page 1 and 2: Studies in Mycology 75: 213-305.A n
Page 3 and 4: A new approach to species delimitat
Page 15: A new approach to species delimitat
Page 18 and 19: Verkley et al.CaryophyllaceaeRutace
Page 20 and 21: Verkley et al.CaryophyllaceaeRutace
Page 22 and 23: Verkley et al.Fig. 3. Caryophyllose
Page 38 and 39: Verkley et al.wk (15-18 mm in 7 wk)
Page 40 and 41: Verkley et al.Conidiomata simple or
Page 42 and 43: Verkley et al.Fig. 16. Septoria cuc
Page 44 and 45: Verkley et al.Fig. 17. Septoria dig
Page 46 and 47: Verkley et al.Baarnsche bos, ex lea
Page 48 and 49: Verkley et al.Septoria galeopsidis
Page 50 and 51: Verkley et al.already considered th
Page 52 and 53: Verkley et al.Fig. 23. Septoria lac
Page 54 and 55: Verkley et al.much more profound di
Page 56 and 57: Verkley et al.Fig. 26. Septoria lyc
Page 58 and 59: Verkley et al.Fig. 27. Septoria lys
Page 60 and 61: Verkley et al.to very dark dull gre
Page 62 and 63: Verkley et al.Fig. 30. Septoria nap
Page 64 and 65: Verkley et al.Fig. 31. Septoria par
Page 66 and 67: Verkley et al.Fig. 32. Septoria pet
Page 68 and 69: Verkley et al.Fig. 34. Septoria pol
Page 70 and 71: Verkley et al.CBS 179.77; same area
Page 72 and 73: Verkley et al.0(-9-11?)-septate con
Page 74 and 75: Verkley et al.to 60 µm diam, lacki
Page 76 and 77: Verkley et al.Fig. 38. Septoria sis
Page 78 and 79: Verkley et al.on OA, but olivaceous
Page 80 and 81: Verkley et al.Fig. 41. Septoria urt
Page 82 and 83:
Verkley et al.ampulliform to almost
Page 84 and 85:
Verkley et al.diam; Conidiogenous c
Page 86:
Verkley et al.Notes: The type mater
Page 89 and 90:
A new approach to species delimitat
Page 91 and 92:
Page 93:
show all

A new approach to species delimitation in Septoria - CBS - KNAW

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?