A new approach to species delimitation in Septoria - CBS - KNAW

Verkley et al.Fig. 38. Septoria sisyrinchii, CBS 112096. A–D. Colonies (15 °C, nUV). A. On OA. B. Ibid., reverse. C. On MEA. D. Ibid., detail of colony margin. E–G. Conidia on OA. Scalebars = 10 µm.the truncate base, (0–)1–3-septate, not constricted aroundthe septa, hyaline, containing minute oil-droplets and granularmaterial in the rehydrated state, (15.5–)20–30 × 1.5–2(–2.5) µm(rehydrated). Sexual morph unknown.Description in vitro (18 ºC, near UV): Colonies on OA 11–15 mm diamin 2 wk, with an even, buff margin; colonies restricted to spreading,immersed mycelium a mixture of luteous and saffron, the surfaceprovided with a very diffuse, white fluffy to woolly aerial mycelium,which is denser in zones; reverse sienna; numerous conidiomatadeveloping after 5–7 d especially in the centre, releasing milkywhite rosy-buff conidial slime. Colonies on MEA 10–14 mm diamin 2 wk, with a buff, minutely ruffled margin; colonies restricted,radially striate and somewhat elevated in the centre, the surfacedirty greyish brown, soon covered by large masses ochreous topale brown masses of conidia. Reverse chestnut to blood color, orbrown-vinaceous.Conidiomata and conidiogenous cells as in planta. Conidia asin planta, mostly 18–35× 1.5–2.5 µm.Hosts: Sisyrinchum spp.Material examined: New Zealand, Auckland, Manurewa, Auckland BotanicalGardens, on leaf of Sisyrinchum sp., 28 Dec. 2002, C. F. Hill LYN 755, CBSH-21259, living culture CBS 112096.Notes: The material from Auckland agrees well with the originaldiagnosis of S. sisyrinchii, which was based on material fromSisyrinchium bonariense in Argentina. Conidia were described as0–3-septate, 15–24 × 2.5 µm. The multilocus phylogeny indicatesthat S. anthurii of the genus Anthurium (Araceae) is a closelyrelated species (Fig. 2).Septoria stachydis Roberge ex Desm., Annls Sci. Nat., sér.3, Bot. 8: 19. 1847. Fig. 39.Description in planta: Symptoms leaf spots angular or irregular,greyish to yellowish brown, with a somewhat darker to black border.Conidiomata pycnidial, epiphyllous, rarely also hypophyllous,mostly 1–5 in each leaf spot, globose to subglobose, dark brown,semi-immersed, 65–100(–125) µm diam; ostiolum central,circular, 12–20 µm wide, later opening more widely up to 50 µm,surrounding cells somewhat darker; conidiomatal wall 12–18 µmthick, composed of angular and irregular cells 2.5–6 µm diam,the outer cells with brown, somewhat thickened walls, the innercells with hyaline and thinner walls. Conidiogenous cells discrete,sometimes integrated into 1–septate conidiophores, hyaline,broadly ampulliform with a relatively narrow neck, holoblastic,proliferating percurrently with indistinct annellations, rarely alsosympodially, 5–8(–10) × 2.5–3.5(–5) µm. Conidia filiform to filiformcylindrical,curved or irregularly bent, rarely straight or flexuous,with a narrowly rounded or somewhat pointed apex, with a truncatebase, (0–)1–3(–5)-septate, not constricted around the septa,hyaline, contents with several minute oil-droplets and granularmaterial in each cell in the living state, with inconspicuous oildropletsand granular contents in the rehydrated state, (17–)20–42 × 1–2 µm (living; rehydrated, 1–1.5 µm wide). Sexual morphunknown.Description in vitro: Colonies on OA 13–16 mm diam in 2 wk(V1049: 8–10 mm in 12 d, 16–18 mm in 3 wk; > 50 mm in 7 wk),with an even, glabrous, colourless margin; immersed myceliummostly homogeneously coral after 2 wk, the centre of thecolony already appearing almost black by numerous superficialand immersed pycnidia; olivaceous-black sectors with dark288