A new approach to species delimitation in Septoria - CBS - KNAW

More documents

Recommendations

Info

Verkley et al.Fig. 46. Sphaerulina tirolensis. A. Conidia in planta (CBS H-21232, holotype). B. Conidia on OA (CBS 109017). Scale bars = 10 µm.mat of white or grey aerial mycelium; a diffusable pigment stainingthe surrounding agar more or less ochreous; reverse usually darkumber or olivaceous-black in the centre, surrounded by ochreous,which later becomes fulvous to apricot. Colonies on CHA 3–4 mmdiam in 2 wk, 5–6 mm in 3 wk (12–16 mm in 7 wk), with an evenbut later more irregular, glabrous, buff, rosy-buff or flesh margin;colonies pustulate to almost hemispherical, the surface olivaceousblackto dark slate blue, glabrous, or covered by diffuse, greyish orflesh aerial mycelium, some colonies later covered by a pure white,dense mat of aerial mycelium; diffusable pigment not observed;reverse blood colour to umber. Cultures produce large masses ofpale flesh conidial slime, aggregating around the colony margin.Conidiomata pycnidial or merged into stromatic complexes.Conidiogenous cells as in planta. Conidia straight to curvedor flexuous, narrowly to broadly rounded at the apex, narrowlytruncate at the base, 3–7(–9)-septate, not constricted around thesepta, hyaline, contents granular with minute oil-droplets, 54–96(–108) × 2.5–3 µm.Host: Rubus idaeus.Material examined: Austria, Tirol, Pitztal, Arzl, on living leaves of Rubus idaeus, 30July 2000, G. Verkley 1021, holotype CBS H-21232, living cultures ex-type CBS109017, 109018.Notes: Sphaerulina tirolensis differs from another septoria-likefungus described on R. idaeus, viz. Rhabdospora rubi var. rubiidaeidescribed from stems of R. idaeus in Romania, with conidia(36–)40–50(–60) × 2(–2.5) µm. Demaree & Wilcox (1943) studiedSeptoria leaf-spot diseases of raspberry (R. idaeus) in NorthAmerica. Cylindrosporium rubi, of which the sexual morph isSphaerulina rubi cf. Demaree & Wilcox (1943), is also different.The sequences of the various protein-coding genes fully supportSphaer. tirolensis as a separate species from the next taxon,Sphaer. westendorpii. The latter can be distinguished from Sphaer.tirolensis by the smaller conidia in planta [24–45(–50) × 1.8–2.2µm] and also in culture [30–68(–80) × 1.5–2(–2.5) µm].Sphaerulina westendorpii Verkley, Quaedvlieg & Crous,comb. et nom. nov. MycoBank MB804480. Fig. 47.Basionym: Septoria rubi Westend., in Westend. & Wallay, Herb.crypt. Belge, Fasc. 19, no. 938. 1854; Kickx, Fl. crypt. Flandr. 1:432. 1867.= Mycosphaerella rubi Roark, Phytopathology 11: 329. 1921.Description in planta: Symptoms leaf lesions numerous, circularto irregular, single or confluent, pale yellowish brown to greyishbrown, partly well-delimited by a dark red brown line or zone.Conidiomata pycnidial, epiphyllous, several in each lesion,immersed, subglobose to globose, brown to black, 55–90 µmdiam; ostiolum central, circular, initially 20–40 µm wide, laterbecoming more irregular and up to 70 µm wide, surrounding cellssomewhat darker; conidiomatal wall 10–15 µm thick, composed ofa homogenous tissue of hyaline, angular cells 2.5–3.5 µm diam,the outermost cells pale brown with slightly thickened walls, theinner cells thin-walled. Conidiogenous cells hyaline, discrete,rarely integrated in 1-septate conidiophores, narrowly to broadly300
A new approach to species delimitation in SeptoriaFig. 47. Sphaerulina westendorpii. A. conidia in planta (CBS H-21229, epitype); B. conidia on OA (CBS 102327). Scale bars = 10 µm.ampulliform, holoblastic, proliferating percurrently several timeswith indistinct annellations thus forming a relatively elongated neck,rarely also sympodially, 5–10(–15) × 2.5–3.5(–4) µm. Conidiafiliform-cylindrical, straight, slightly curved to flexuous, narrowlyto broadly rounded at the apex, narrowly truncate at the base,(0–)2–3(–5)-septate, not constricted around the septa, hyaline,contents granular material, sometimes with minute oil-droplets bothin the living and rehydrated state, 24–45(–50) × 1.8–2.2 µm (living;rehydrated, 1.5–2.0 µm wide).Description in vitro: Colonies on OA 8–10 mm diam in 19 d, with aneven, glabrous, colourless or buff to rosy-buff margin; immersedmycelium dark green or dull green, but sectors or other parts ofcolonies may be only olivaceous-buff or rosy-buff to salmon;colonies spreading, with irregular elevations in the centre on whichconidiomata are formed, releasing a whitish conidial slime; aerialmycelium almost absent to well developed and forming a dense,white, woolly-floccose mat; reverse olivaceous-black, locally buffto rosy-buff. Colonies on CMA 5–7(–10) mm diam in 19 d, as onOA, but more distinctly elevated and restricted. In faster growingsectors salmon to ochreous pigmentation (due to weak productionof red pigment?) in a peripheral zone preceedes the formationa dominant greens. Conidial slime also milky white, as on OA.Colonies on MEA 9–12 mm diam in 19 d, with an even, glabrouscolourless to buff margin; colonies restricted, irregularly pustulate tohemispherical; immersed mycelium buff to honey near the margin,olivaceous-black in the centre, sometimes mostly honey; almostentirely covered by a dense, appressed mat of white or grey aerialmycelium; a diffusable pigment staining the surrounding agar moreor less ochreous; reverse usually dark umber or olivaceous-black inthe centre, surrounded by ochreous, which later becomes fulvousto apricot. Colonies on CHA 7–9 mm diam in 19 d, with an evenbut later more irregular, glabrous, buff, rosy-buff or flesh margin;colonies pustulate to almost hemispherical, the surface ochreousto sienna, glabrous, or covered by diffuse, greyish or flesh aerialmycelium; diffusable pigment not observed; reverse blood colourto umber.Conidiomata pycnidial or merged into stromatic complexes,as in planta. Conidiogenous cells as in planta, mostly cylindricaland proliferating percurrently, rarely also sympodially, 7–15(–18) ×2.5–3.5(–4) µm; Conidia as in planta but mostly 3–5-septate andconsiderably longer, 30–68(–80) × 1.5–2(–2.5) µm.Hosts: Rubus spp.Material examined: Belgium, Oostacker, near Gand, on leaves of Rubus sp.,isotype BR-MYCO 159265-88, also distributed in Westend. & Wallay, Herb. crypt.Belge, Fasc. 19, no. 938. Czech Republic, Mikulov, on living leaves of Rubussp., 15 Sep. 2008, G. Verkley 6002, CBS H-21257. Netherlands, prov. Limburg,Gerendal, on living leaves of R. fruticosus s.l., 28 June 2000, G. Verkley 964,epitype designated here CBS H-21229 “MBT175362”, living cultures ex-epitypeCBS 109002, 109003; Prov. Limburg, Mookerheide, in mixed forest, on living leavesof R. fruticosus s.l., 9 Sep. 1999, G. Verkley 923, CBS H-21205, living culture CBS102327; same loc. and substr., 23 Aug. 2004, G. Verkley & M. Starink 3036, CBSH-21263, living culture CBS 117478; same substr., Prov. Limburg, St. Jansbergnear Plasmolen, in mixed forest, G. Verkley 924, CBS H-21206; Prov. Flevoland,Erkemeder strand, in sandy dunes, on living leaves of R. fruticosus s.l., 8 Sep. 1999,G. Verkley 930, CBS H-21210.Notes: Jørstad (1965) discussed the problems regarding thetaxonomy of Septoria species described from Rubus. Some of thelater described taxa have been placed in synonymy with Septoriarubi, but most still need to be reevaluated based on fresh material,culture studies, and molecular characterisation. The type materialin BR contains several well-preserved leaves of the R. fruticosuscomplex, showing typical symptoms. Fruitbodies investigatedcontained mostly 1–3-septate conidia, 17.5–40 × 1–1.5 µm, andwith the typical shape of this common fungus on Rubus spp. Thespecimen CBS H-21229 from R. fruticosus in the south of theNetherlands, is chosen as epitype. This species is nested withinthe Sphaerulina-clade, and a new name in Sphaerulina shouldwww.studiesinmycology.org301
Page 1 and 2:
Studies in Mycology 75: 213-305.A n
Page 3 and 4:
A new approach to species delimitat
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15:
Page 18 and 19:
Verkley et al.CaryophyllaceaeRutace
Page 20 and 21:
Verkley et al.CaryophyllaceaeRutace
Page 22 and 23:
Verkley et al.Fig. 3. Caryophyllose
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35:
Page 38 and 39: Verkley et al.wk (15-18 mm in 7 wk)
Page 40 and 41: Verkley et al.Conidiomata simple or
Page 42 and 43: Verkley et al.Fig. 16. Septoria cuc
Page 44 and 45: Verkley et al.Fig. 17. Septoria dig
Page 46 and 47: Verkley et al.Baarnsche bos, ex lea
Page 48 and 49: Verkley et al.Septoria galeopsidis
Page 50 and 51: Verkley et al.already considered th
Page 52 and 53: Verkley et al.Fig. 23. Septoria lac
Page 54 and 55: Verkley et al.much more profound di
Page 56 and 57: Verkley et al.Fig. 26. Septoria lyc
Page 58 and 59: Verkley et al.Fig. 27. Septoria lys
Page 60 and 61: Verkley et al.to very dark dull gre
Page 62 and 63: Verkley et al.Fig. 30. Septoria nap
Page 64 and 65: Verkley et al.Fig. 31. Septoria par
Page 66 and 67: Verkley et al.Fig. 32. Septoria pet
Page 68 and 69: Verkley et al.Fig. 34. Septoria pol
Page 70 and 71: Verkley et al.CBS 179.77; same area
Page 72 and 73: Verkley et al.0(-9-11?)-septate con
Page 74 and 75: Verkley et al.to 60 µm diam, lacki
Page 76 and 77: Verkley et al.Fig. 38. Septoria sis
Page 78 and 79: Verkley et al.on OA, but olivaceous
Page 80 and 81: Verkley et al.Fig. 41. Septoria urt
Page 82 and 83: Verkley et al.ampulliform to almost
Page 84 and 85: Verkley et al.diam; Conidiogenous c
Page 86: Verkley et al.Notes: The type mater
Page 91 and 92: A new approach to species delimitat
Page 93: A new approach to species delimitat
show all

A new approach to species delimitation in Septoria - CBS - KNAW

Create successful ePaper yourself

Delete template?

Save as template?