A new approach to species delimitation in Septoria - CBS - KNAW

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Verkley et al.Fig. 23. Septoria lactucae. A–D. Colonies CBS 108943 (15 °C, nUV). A. On OA. B. On MEA. C. Colony margin on OA. D. Colony margin on MEA. E–I. Conidia on OA (CBS108943). Scale bars = 10 µm.neck, holoblastic, proliferating sympodially, and towards the apexoften also percurrently 1–many times with indistinct annellations,5–10(–12) × 3.5–4(–5) µm. Conidia filiform to filiform-cylindrical,straight or slightly curved, rarely flexuous, with a rounded orsomewhat pointed apex, attenuated towards the narrowly truncatebase, (0–)3(–5)-septate, not constricted around the septa, hyaline,contents with several minute oil-droplets and granular material ineach cell in the living state, with inconspicuous oil-droplets andgranular contents in the rehydrated state, (26–)35–50(–54) × 1.5–2.5( –3) µm (living; rehydrated, 1–2 µm wide; V1032, rehydrated,33–52 × 1.5–2). Sexual morph unknown.Description in vitro: Colonies on OA 8–14 mm diam in 2 wk (40–45mm in 6 wk), with an even, glabrous, colourless margin; coloniesplane, immersed mycelium colourless to pale primrose or buff, laterbecoming homogeneously dark herbage green, soon appearingdarker by numerous immersed and superficial pycnidial conidiomata,that release dirty white to rosy-buff conidial slime; aerial myceliumabsent, only developing in the centre after several wk as a sharplydelimited, dense, white, woolly floccose mat; reverse buff at themargin, inwards dark olivaceous-grey. Colonies on CMA 4–8 mmdiam in 2 wk (20–27 mm in 6 wk), with an even, glabrous margin;as on OA but immersed mycelium more honey to pale luteousthroughout, later becoming more greenish, the pycnidial conidiomataas on OA, but in more regular concentric rings, releasing rosy-buff,later salmon conidial slime. Colonies on MEA 7–9 mm diam in 2wk (28–33 mm in 6 wk), with an even (later undulating), glabrous,buff to honey margin; colonies pustulate to almost hemispherical,immersed mycelium rather dark, locally covered by woolly to feltywhite aerial mycelium; mostly composed of spherical conidiomatal264
A new approach to species delimitation in SeptoriaFig. 24. Septoria lamiicola, CBS 102329. A–C. Colonies (15 °C, nUV). A. On OA. B. On CHA. C. On MEA. D. Conidia in planta (CBS H-21181). E. Ibid. (CBS H-21216). F.Conidia and conidiogenous cells on OA (CBS 102380). Scale bars = 10 µm.initials, superficial mature conidiomata releasing a dirty white, laterbuff conidial slime; reverse dark brick in the centre, near the margincinnamon to honey. Colonies on CHA 8–14 mm diam in 2 wk (35–42mm in 6 wk), with an even, but later irregular, buff margin coveredby a diffuse, felty white aerial mycelium; further as on MEA, but thecolony surface less elevated, and more homogeneously coveredby diffuse, felty, white aerial mycelium; conidial slime abundantlyproduced, first milky white, later dirty honey; reverse in the centreblood colour, dark brick at the margin.Conidiomata pycnidial, first olivaceous, then almostblack, glabrous, 150–450 µm diam, with 1–5 ostioli placed onshort papillae or more elongated necks up to 350 µm long;conidiogenous cells as in planta, proliferating sympodially andmostly also percurrently with distinct annellations, 8–16 × 3–8µm; conidia cylindrical, straight or slightly curved, tapering to arounded apex, lower part slightly attenuated into a broad truncatebase, (0–)1–5-septate, not constricted around the septa, hyaline,with several oil-droplets and minute granular material in each cell,(34–)50–65(–70) × 2–3 µm.Hosts: Lamium album, L. maculatum, L. purpureum and severalother Lamium spp.Material examined: Austria, Tirol, Ötztal, Sulztal, Gries, alt. 1570 m, on living leavesof Lamium album, 1 Aug. 2000, G. Verkley 1032, CBS H-21181, living culturesCBS 109112, 109113. Czech Republic, Moravia, Pavlov, forest around ruin, onliving leaves of Lamium sp., 18 Sep. 2008, G. Verkley 6020, CBS H-21251, livingcultures CBS 123882, 123883, and 6021, CBS H-21252, living culture CBS 123884.Netherlands, prov. Limburg, St. Jansberg near Plasmolen, on living leaves of L.album, 9 Sep. 1999, G. Verkley 925, CBS H-21207, living cultures CBS 102328,102329; prov. Gelderland, Millingen aan den Rijn, Millingerwaard, on living leaves ofL. album, 6 Oct. 1999, G. Verkley 936, CBS H-21216, living cultures CBS 102379,102380.Notes: According to Jørstad (1965), conidia of S. lamiicola are3-septate, 24–60 × 1–2 µm, while Teterevnikova-Babayan (1987)reported 35–50 × 0.75–1.5 µm from seven Lamium species.For the current study, only fresh material on Lamium album wasavailable. Jørstad (1965) mentioned the resemblance of theconidia with those in S. galeopsidis, but also noted a differencein the wall thickness of the pycnidia, which we did not observe. Awww.studiesinmycology.org265
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Page 18 and 19: Verkley et al.CaryophyllaceaeRutace
Page 20 and 21: Verkley et al.CaryophyllaceaeRutace
Page 22 and 23: Verkley et al.Fig. 3. Caryophyllose
Page 38 and 39: Verkley et al.wk (15-18 mm in 7 wk)
Page 40 and 41: Verkley et al.Conidiomata simple or
Page 42 and 43: Verkley et al.Fig. 16. Septoria cuc
Page 44 and 45: Verkley et al.Fig. 17. Septoria dig
Page 46 and 47: Verkley et al.Baarnsche bos, ex lea
Page 48 and 49: Verkley et al.Septoria galeopsidis
Page 50 and 51: Verkley et al.already considered th
Page 54 and 55: Verkley et al.much more profound di
Page 56 and 57: Verkley et al.Fig. 26. Septoria lyc
Page 58 and 59: Verkley et al.Fig. 27. Septoria lys
Page 60 and 61: Verkley et al.to very dark dull gre
Page 62 and 63: Verkley et al.Fig. 30. Septoria nap
Page 64 and 65: Verkley et al.Fig. 31. Septoria par
Page 66 and 67: Verkley et al.Fig. 32. Septoria pet
Page 68 and 69: Verkley et al.Fig. 34. Septoria pol
Page 70 and 71: Verkley et al.CBS 179.77; same area
Page 72 and 73: Verkley et al.0(-9-11?)-septate con
Page 74 and 75: Verkley et al.to 60 µm diam, lacki
Page 76 and 77: Verkley et al.Fig. 38. Septoria sis
Page 78 and 79: Verkley et al.on OA, but olivaceous
Page 80 and 81: Verkley et al.Fig. 41. Septoria urt
Page 82 and 83: Verkley et al.ampulliform to almost
Page 84 and 85: Verkley et al.diam; Conidiogenous c
Page 86: Verkley et al.Notes: The type mater

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