Dubai Final-v20.indd - World Allergy Organization

ABstrACts
ABstrACts
1316
CHLAMYDOPHILA PNEUMONIAE-inFECTED HUman PEriPHEral mononUClEar CEllS rESiST CorTiCoSTEroiD- inDUCED
SUPPrESSion oF mETalloProTEinaSE-9 anD TiSSUE inHiBiTor mETalloProTEinaSE-1 SECrETion
Park, C. s. 1 , Kim, t. 2 , moon, K. 3 , Bae, Y. 2 , lee, Y. s. 2 , Jang, m. K. 3 , moon, H. 2 and Cho, Y. s. 2
1 2 Department of internal medicine, inje university Heaundae Paik Hospital, Busan, south Korea. Department of Allergy and Clinical
immunology, Asan medical Center, University of Ulsan College of medicine, seoul, south Korea. 3Asan institute for life science,
seoul, south Korea.
Background: it has been suggested that Chlamydophila pneumoniae infection contributes to the development of severe asthma.
the major characteristics of severe asthma include a reduced response to corticosteroid treatment and progressive airway
remodeling in which an imbalance of metalloproteinase-9 (mmP-9) and tissue inhibitor metalloproteinase-1 (timP-1) is believed
to have an important role. We hypothesized that C. pneumoniae infection affects the secretion of mmP-9 and timP-1 and induces
altered responsiveness to corticosteroids in inflammatory cells.
methods: Human peripheral blood mononuclear cells (PBmCs) were cultured in vitro in the presence or absence of C. pneumonia
infection. Dexamethasone was used in each experiment to assess the responsiveness to the corticosteroid. the values of secreted
mmP-9 and timP-1 were measured by ElisA. to evaluate the underlying mechanism, the expression of human glucocorticoid
receptor (gr)-β, known as an endogenous antidote for gr, was observed in PBmCs with or without C. pneumoniae infection using
immunohistochemistry.
results: the secretion of mmP-9 and timP-1 was remarkably suppressed by corticosteroid treatment in PBmCs without
C. pneumoniae infection. in C. pneumoniea-infected PBmCs, the suppressed secretion of mmP-9 by the corticosteroid was
significantly inhibited, while the level of timP-1 secretion did not change compared with those levels in untreated PBmCs.
therefore, the molar ratio of secreted mmP-9/timP-1 was decreased by C. pneumonia infection and was more exaggerated under
corticosteroid treatment. the expression of gr-β was significantly increased in C. pneumoniae-infected PBmCs.
Conclusion: C. pneumoniae infection in inflammatory cells may induce altered secretion of tissue enzymes associated with airway
remodeling through a decreased responsiveness to corticosteroids and may be linked to the pathogenesis of severe asthma.
Key words: Asthma, Chlamydophila pneumoniae, Peripheral blood mononuclear cells, mmP-9, timP-1, glucocorticoid receptor (gr)
- β
1317
EFFECTS oF TraFFiC air PollUTion on rESPiraTorY HEalTH anD allErgiES in EgYPTian SCHoolCHilDrEn
shamssain, m. 1 , Al Qerem, W. 2 , mcgarry, K. 1 and neshat, l. 1
1 2 Pharmacy, Health and Wellbeing, University of sunderland, U.K, sunderland, United Kingdom. Pharmacy, Health and Wellbeing,
University of sunderland, sunderland, United Kingdom.
Background
studies suggest that traffic exposures can influence asthma and allergic symptoms among children; air pollution is associated with
exacerbation of asthma symptoms in children with asthma. there has been few studies about the susceptibility of subgroups and
on new onset asthma.the objective of the study is to investigate the effects of traffic air pollution on allergies with emphasis on
gender differences in the respiratory effects of air pollution.
methods
We studied 1400 schoolchildren from two locations in Egypt: Cairo with high level of air pollution and shbeen Al Koom in the
Delata with low level of air pollution. lung function testing was done by the Vitalograph spirometer. the Arabic version of isAAC
questionnaire was used (the international study of Asthma and Allergies in Childhood). Air pollution measurements were collected
from the government sites in both locations.
results
mean values of sO2, nO2, Ozone, CO, and Pm10 in Cairo were significantly lower than shbeen Al Koom (39.0 vs 17.0;62.8 vs
55.8 ug/m3; 93.0 vs 28.8 ug/m3; 3.0 vs 1.3 mg/m3; and 263.5 vs 94.0 ug/m3, respectively. Forced vital capacity (FVC), forced
expiratory volume in the first second (FEV1) and peak expiratory flow rate (PEFr) were lower in children in Cairo compared to shben
Al Koom (1.99 vs 2.03 l; 1.70 vs 1.79 l; and 204.4 vs 207.4 l/min, respectively. the prevalence rates of current wheeze, asthma,
rhinitis, hay fever and eczema in Cairo were higher than shbeen Al Koom (6.9 vs 5.6% ; 5.5 vs 3.3%; 24.2 vs 17.9; 9.5 vs 6.4%;
and 9.1 vs 5.6% , respectively. the prevalence rates of ever rhinitis were 6.0% and 3.0% higher in boys and girls in Cairo compared
to shbeen Al Koom, respectively. Children who developed rash less than 2 years of age were 2.0% and 5.0% higher in boys and
girls in Cairo compared to shbeen Al Koom, respectively.
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