Dubai Final-v20.indd - World Allergy Organization

ABstrACts
1326
analYSiS oF gEnE EXPrESSion ProFilES oF PEriPHEral BlooD B lYmPHoCYTES in VarioUS CliniCal PHEnoTYPES oF
aSTHma
Ahmad, n. E. Q. 1 , Harun, r. 2 and Othman, r. 2
1 2 institute of medical science technology (mEstECH), Universiti Kuala lumpur (UniKl), Kajang, malaysia. UKm medical molecular
Biology institute (UmBi), Universiti Kebangsaan malaysia (UKm), Cheras, malaysia.
Background B lymphocytes play important roles in the inflammatory response in asthma including igE production, antigen
presentation and secretion of certain cytokines. However the exact molecular pathways are still largely unknown. the objectives of
this study were to identify gene expression profiles of peripheral blood B lymphocytes and subsequently determine gene signatures
that were associated with different clinical phenotypes of asthma. in addition we aimed to identify biological processes and
pathways involved in the molecular mechanisms of asthma.
method Peripheral blood B cells were isolated from subjects with different manifestations of asthma: mild (n=8), moderate
controlled (n=8), moderate uncontrolled (n=10), severe steroid dependant (n=7), severe steroid resistant (n=7) and normal
control (n=8). total rnA were extracted from the B lymphocytes, reverse transcribed and amplified linearly before being labelled
and hybridized on illumina Humanref-8 Expression BeadChips that had 24,355 elements. microarray data was analysed using
genespring gX 10.0.02 software and real-time PCr was performed to validate the microarray gene expression.
results Data were analysed according to several conditions including asthma control status, steroid response, asthma severity and
disease status. Differential gene analyses, corrected for multiple testing, revealed 7 (p