Dubai Final-v20.indd - World Allergy Organization

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smaller than 100 nm. Cell cycle and apoptosis were performed by flow cytometry. After 72hrs treatment, the viability decreased
slightly related with the increasing ranges of size of the metal fume particles, counted by trypan Blue stain and Propidium iodide
(Pi) uptake, and apoptosis was estimated by cytosolic caspase 3 activation. the rates of apoptosis in each treatment were about
7~12% of the control. the increase rate of caspase 3, is according to the order of particle size reduction. Different size of welding
fume particles also affect sub g cell cycle from 1~9 %, and 23~26% of g /m stage of the total cells. With the reduction of particle
1 2
size, we observed a decrease tendency of sub g1 phase and an increase tendency of g /m phase in total cell percentages.
2
We conclude nano-sized particles in metal fume increases the production of inflammatory cytokines of t cell in a short term
treatment, and makes cells arrested at g /m phase and increases cell apoptosis in a long term exposure. statistical results support
2
the ultra-fine and nano-scale particles in welding fume cause larger effects on inflammatory cytokine production, Caspase 3 and
g /m arrest (r = -0.425, P= 0.019). some metals, such as manganese, Copper (Cu) and nickel were analyzed by Atomic Absorption,
2
only the concentrations of smaller sized Cu particles exhibited significant correlation with Pi (r=-0.20, P=0.04). the mechanisms
induce production of cytokines; apoptosis and g2 arrest with nano-sized welding fume remain to be clarified in future.
3105
THE EFFiCaCY oF a naSal anTiHiSTaminE oloPaTaDinE For THE TrEaTmEnT oF SEroUS oTiTiS mEDia in CHilDrEn
nsouli, s.
Asthma and Allergy, DAnVillE AstHmA AnD AllErgY CliniC, DAnVillE, CAliFOrniA, UsA, Danville, CA.
Previously we have shown that the combination of a nasal Corticosteroid mometasone with an oral antibiotic may be more
efficacious than monotherapy with an oral antibiotic in the treatment of serous otitis media in children.
since chronic inflammation is the histopathologic landmark of otitis media with effusion, clinical observations have led us to believe
that the combination of a nasal Antihistamine Olopatadine with an oral antibiotic may be more effective than monotherapy with an
oral antibiotic in the treatment of serous otitis media.
We studied forty pediatric patients (age 6 years to 11 years) in a randomized open labeled 2-week trial to compare the efficacy of
the combination nasal Olopatadine 665 mcg/nostril twice daily with an oral antibiotic Amoxicillin/Clavulanate potassium (90mg/kg/
day in 2 divided doses every 12 house) for the treatment of otitis media with effusion
the efficacy of the treatment options was assessed using pneumatic otoscopy, impedance tympanometry, and audiometry to
monitor the clinical course of the middle ear effusion in both treatment groups.
in the combination group nasal Olopatadine and oral antibiotic a resolution of otitis media with effusion occurred at the 7th day. in
contrast in the group treated with monotherapy with the oral antibiotic the resolution of otitis media with effusion occurred on the
14th day.
in conclusion, the combination of nasal antihistamine Olopatadine plus an oral antibiotic is more effective than monotherapy with an
oral antibiotic. the combination nasal antihistamine Olopatadine plus an oral antibiotic may be a safer and shorter therapy given the
safety issues with long term use of system antibiotics.
3106
ComBinaTion oF a naSal anTiHiSTaminE oloPaTaDinE anD a naSal CorTiCoSTEroiD, momETaSonE For THE
TrEaTmEnT oF SEaSonal allErgiC rHiniTiS PaTiEnTS noT CUrrEnTlY ConTrollED on monoTHEraPY inTranaSal
anTiHiSTaminE or inTranaSal CorTiCoSTEroiD
nsouli, s.
Asthma and Allergy, DAnVillE AstHmA AnD AllErgY CliniC, CAliFOrniA, UsA, Danville, CA.
For seasonal Allergic rhinitis (sAr) patients that remain symptomatic on an intranasal antihistamine, Olopatadine or intranasal
corticosteroid, mometasone furoate, the combination of intranasal antihistamine, Olopatadine with an intranasal cortiscosteroid
mometasone may provide additional efficacy in sub-optimally controlled seasonal Allergic rhinitis Patients.
in this open labeled 8-week trial 40 patients with symptomatic sAr currently using Olopatadine 1330 mgc/nostril BiD or
mometasone furoate, 100 mcg/nostril QD were randomized to receive the combination Olopatadine 1330 mcg/nostril BiD +
mometasone, 100 mcg/nostril QD. the end points of the trial include: rhinomanometry, nasal symptom score (composite score of
nasal congestion, rhinorrhea, sneezing post nasal drip and itching) and flexible rhinopharyngolaryngoscopy examination.
mean efficacy measurements at the end of the 8-week trial revealed significant improvements in all parameters examined in the
combination treatment group as compared to baseline measurements.
in conclusion, the combination nasal Olopatadine plus nasal mometasone is more effective than monothereapy nasal Olopatadine
or nasal mometasone. it appears that in the combination treatment Olopatadine and mometasone, the primary end points
(rhinomanometry and symptom scores) are significantly improved.
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