Dubai Final-v20.indd - World Allergy Organization

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3107
EXPErimEnTallY UnraVEling THE aToPiC marCH
Wilson, m. s.
molecular immunology, nimr, mrC, london, United Kingdom.
Experimentally Unraveling the atopic march.
mark s. Wilson. molecular immunology Division. national instiutes for medical research, mrC. mill Hill. london. UK.
the sequential progression of allergic symptoms from atopic eczema, food allergy, rhinitis and asthma have been widely reported
and referred to as ‘the Atopic march’. Co-morbidities of allergic conditions and the over-dispersed distribution of allergic diseases
within few individuals have also been identified. these data suggest that allergic diseases may be linked; however the relationships
between allergic symptoms are unclear. to separate cause from consequence, we are investigating the immunological interactions
between these disease phenotypes and have developed experimental models of allergic asthma, allergic rhinitis and food allergy.
these models are used to ask whether food allergy, and/or upper airway rhinitis influence the development of lower airway
asthma. mice sensitized and orally challenged with crude peanut antigen (CPA) are subsequently sensitized and challenged, in the
lower airways, with house dust mite (HDm). A cohort of peanut-allergic mice will also be sensitized and challenged in the upper
airways with ragweed (rW) prior to lower airway HDm challenge. We hypothesize that peanut allergy and rW-induced rhinitis will
exacerbate HDm-sensitization (igE) and HDm-induced airway inflammation. the molecular mechanisms of such interactions will
be investigated and tested. Our studies and latest observations will be presented. This work is supported by the Medical Research
Council (MRC), UK.
3108
a noVEl rolE For THE ComPlEmEnT rEgUlaTor CD46 in EPiTHElial TigHT JUnCTion FormaTion/rEgUlaTion
Al-shouli, s. t. 1 and Kemper, C. 2
1 2 Clinical immunology and Allergy., King’s College london guy’s and s’t thomas’ Hospital, london, United Kingdom. mrC Centre for
transplantation, london, United Kingdom.
Background: the complement regulator CD46 has been shown to play a role in epithelial cell polarization and is overexpressed on
epithelial tumor cells. it is clear that our understanding of the many functions of the complement system in health is growing but
still not complete. it is therefore to be expected that complement will be connected in the future with additional human diseases.
this research project focuses on just such a prediction: We have obtained data suggesting a novel role for CD46 in epithelial cell
tight junction regulation and growth induction or restriction. these data suggest that complement may also play a previously
unacknowledged role in another important human disease, colon cancer. Colon cancer is the third most common cancer in the
UK with a poor prognosis because of a high mortality and recurrence rate, thus, there is a need to develop better treatments.
methods: 1-Culture PtECs or Caco-2 cells for several days on 24 wells-plates to measure E-cadherin expression, CD46 expression,
Cell-proliferation assessed and apoptosis induced in cells. 2- Culture the human kidney proximal tubular epithelial cells (PtECs)
or the human intestinal cell line (Caco-2) for several days on transwells to measure transepithelial resistance (tEr) and Beads
dextran. results: we found that CD46 interacts with E-cadherin and sPAK, both vital proteins in the maintenance of epithelial cell
layer integrity. mutations in either protein cause colon cancer or iBD, respectively. Further, we observed that CD46 regulates tight
junctions and by this transepithelial resistance and paracellular permeability. Based on these data we hypothesize that complement/
CD46 communicates with the E-cadherin/catenin network in epithelial cells (via interaction/activation of sPAK) and contributes to
normal epithelial cell barrier integrity. Further, defects in CD46-mediated signals leading to disturbance in its crosstalk with the
E-cadherin/catenin network may be a factor in malignant transformation. Conclusion: our study was conducted to ascertain the
biological properties of CD46 in a cell-cell adhesion network. We believe that this study helped to further solidify this idea and set
the ground for more detailed future studies.
3109
THE rolE oF EXTErnal PHYSiCal ForCES in TriggEring THE allErgiC rEaCTionS
Athota, r. r. 1 , reddy, r. r. K. 2 , neerupudi, K. B. 1 and sam, r. A. 3
1 2 3 Biochemsitry, Andhra University, Visakhapatnam, india. Department of Physics, s K University, Anantapur, india. internal medicine,
Christian medical College, Vellore, india.
Background: in the era of scientific advancements, the allergic population is increasing at an alarming rate in modern urban
society. in this context, the influence of the external forces like humidity and other physical forces in triggering the allergic
symptoms is investigated.
methods: the humidity has been measured in two cities namely Visakhapatnam, a coastal city and Anantapur, a non-coastal
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