Dubai Final-v20.indd - World Allergy Organization

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abscesses are visible at her thigh. there was no other site of local or systemic infection and the general status remained good
during and after gamma- interferon therapy.
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FloW CYTomETrY aS a Tool For DElaYED DrUg allErgY rEaCTionS DiagnoSiS: oUr EXPEriEnCE
Ardito, s. 1 , De Donno, m. 2 , Aiello, V. 2 , iaia, m. l. 1 and maietta, g. 2
1 2 Allergy Department, Perrino Hospital, Brindisi, italy. Cellular immunology, Pignatelli institute, lecce, italy.
introduction.
the lymphocyte transformation test (ltt) is the only test to detect a sensitization of t cells to drugs in the peripheral blood of drugallergic
patients, but it is often considered more of a research than a diagnostic tool. in a pilot study, Pichler et al. evaluated surface
molecule CD69 expression as a marker of t-cell activation in a group of patients with t-cell-mediated drug allergic reactions. CD69
was detectable after 48 hrs by flow cytometry in all patients tested.
Aim of the study.
We analysed 11 clinical cases of adverse drug reactions we observed in our department of allergy. in all cases clinical patterns and
the time of reaction indicated a delayed type of immune mechanism.
the patients underwent clinical evaluation, skin tests for the suspected drug and CD69 analysis with flow cytometry.
methods.
Clinical evaluation was made by allergy specialist doctor in agreement with the questionnaire of EAACi interest group on drug
hypersensitivity. 8 patients experienced maculo-papular rush or generalized urticaria after amoxicillin intake, 1 patient after
levofloxacin and 2 patients after ibuprofen or ketoprofen administration
skin test were performed as described by torres mJ et al. (1).
CD69 analysis were performed with flow cytometry by using monoclonal antibodies anti CD3, CD4, CD69, as described from
Pichler et al. (2) and results were expressed as s.i. (stimulation index).
results.
in the group of 8 patients with delayed adverse reactions after amoxicillin intake, 3 patients showed positive intradermal skin tests
with the drug, while 5 patients were negative. CD69 analisys was positive in 6 of the 8 patients.
the patient with maculo-papular rush after levofloxacine intake showed positive patch tests and positive CD69 analisys.
the 2 patients with adverse reactions to nsAiDs showed negative patch tests, but positive CD69 analisys.
Conclusions.
On the basis of our clinical experience, CD69 measurement seems to be a promising tool to detect drug-reactive t cells in the
peripheral blood of drug allergic patients.
references.
(1) m.J. torres , m. Blanca, J. Fernandez , A. romano, A. Weck, W. Aberer , et al. Diagnosis of immediate allergic reactions to betalactam
antibiotics. Allergy. 2003: 58(10):961-72.
(2) A. Beeler, l. Zaccaria,t. Kawabata, B.O. gerber, W.J. Pichler CD69 upregulation on t cells as an in vitro marker for delayed-type
drug hypersensitivity. Allergy 2008: 63: 181–188
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CliniCal CHaraCTEriSTiCS oF oral TolEranCE inDUCTion oF non-igE mEDiaTED FooD allErgY USing iFn-gamma
lee, sr., J. H. and noh, g.
Department of Pediatrics, school of medicine, Chungnam national University, taejeon, south Korea.
Background: Food allergies are classified as igE-mediated (iFA)and non-igE-mediated (nFA). late eczematous reactions of the skin
were found to be one of typical and diagnostic symptoms of nFA. Clinically, oral immunotherapy for food allergy has been tried to
induce oral tolerance.
Objective: recently, oral immunotherapy for food allergy has been successful in nFA using subcutaneous iFn-gamma injection.
We are to characterize the clinical findings of nFA and the induction of the oral tolerance to food allergen in nFA after oral
immunotherapy using iFn-gamma.
methods: non-igE-mediated food allergies were diagnosed by food challenge, skin prick test and food-specific igE. total 77 nFA
patients with atopic dermatitis that is characterized by late eczematous skin reaction were selected in this study. 37 subjets were
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