Dubai Final-v20.indd - World Allergy Organization

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rhinitis patients in Hospital Universiti sains malaysia (HUsm), malaysia before proceed with evaluation of possible association
between this parameter with severity of allergic rhinitis.
in this cross sectional study, total and specific igE to 28 types of allergens were measured in serum samples from 128 allergic
rhinitis patients. the interviewer guided questionnaire was used to evaluate the severity of symptoms using AriA criteria.
in this study, we found that among the aeroallergens, Dermatogoides farinae (97%) was the most common followed by
Dermatogoides pteronyssinus (83%) and house dust mite (43%). Among the food allergen group, shrimp (28.9%) was the most
common implicated food followed by soybean (26.6%), crab (23.4%), clam (22.7%), wheat (21.9%), peanut (20.3%), yolk egg
(18.8%), cow’s milk (18.0%), citrus mix (18.0%), beef(14.1%), white egg (12.5%), tuna (11.7%) and chicken (11.7%). For fungal
allergen, aspergillus spp (23.4%) was found to be the most common fungal followed by candida spp (20.3%), altenaria spp (16.4%),
mucor spp (14.8%), penicillium spp (12.5%) and clasdosporium spp (10.9%). Cockroach (49.2%) was the most common animal
allergen. Cat (44.5%), dog (43.0%), latex (42.2%), Bermuda (28.9%) and Johnson (21.9%) grass were also common. in this study,
we also found a significant association between the specific igE levels and the severity of allergic rhinitis for Dermatogoides farinae
(p=0.024), Dermatogoides pteronyssinus (p=.039), cockroach (p = 0.038), cat (p = 0.042) and latex (p = 0.044).
in conclusion this study revealed a significant association between specific igE levels and the severity of allergic rhinitis symptoms
for Dermatogoides farinae, Dermatogoides pteronyssinus, cockroach, cat and latex.
1402
Co-aDminiSTraTion oF CHEnoPoDiUm alBUm allErgEnS anD CPg oligoDEoXYnUClEoTiDES EFFECTS on PEriPHEral
BlooD mononUClEar CEllS oF PaTiEnTS WiTH allErgiC rHiniTiS TrEaTED WiTH inTranaSal CorTiCoSTEroiDS anD
anTiHiSTaminES
Farrokhi, sr., s. 1 , mousavi, t. 1 , Arshi, s. 1 , salekmoghadam, A. 1 , Varasteh, A. 2 and rezaei, n. 3
1 2 Department of immunology, iran University of medical sciences, tehran, iran. 6. immunobiochemistry lab, immunology research
Center, Avicenna research institute, 6. immunobiochemistry lab, immunology research Center, Avicenna research institute,
mashhad, iD, iran. 3growth and Development research Center, Children’s medical Center, tehran, iran.
Objective. Allergic rhinitis (Ar) is one of the most common chronic diseases in the developed countries, associated with substantial
economic burden and morbidity. this study was performed to investigate the effect of Cpg-ODn in alteration of t-helper (th)1/th2
balance of patients with Ar treated with intranasal corticosteroids (inCs) and antihistamines.
methods. Peripheral blood mononuclear cells (PBmCs) of 20 patients with Ar were isolated before and after 45 days treatment
with inCs and antihistamines. Cytokine production (il-4, il-10, il-13, iFn-γ) and specific Ch.a igE in response to Cpg-ODn
co-administration of natural chenopodium album (Cpg/Ch.a) or recombinant Ch.a (Cpg/rCh.a) allergen were investigated using
ElisA method. intracellular il-10 was also assessed in CD4 + cells using flow cytometry. For statistical analysis sPss 16 software
packagresults. significant increases in production of iFn-γ and il-10 and a significant decrease in production of il-4 were found
in PBmCs stimulated with either Cpg/Ch.a or Cpg/rCh.a compared to stimulated cells with allergens alone, before and after therapy
of patients. After therapy, only iFn-γ production with Cpg/Ch.a stimulant was significantly increased in comparison with before
treatment (237 vs. 44 pg/ml, p=0.001). iFn-γ and il-10 production with Cpg/rCh.a stimulant was significantly increased after
therapy compared to before (407.6 vs. 109 pg/ml, p=0.01 for iFn-γ; 171.7 vs. 52.6 pg/ml, p=0.008 for il-10), whilst production
of il-4 was significantly decreased (2.1 vs. 5.8 pg/ml, p=0.02). intracellular il-10 expression was also significantly increased
in response to either Cpg/Ch.a or Cpg/rCh.a. Altogether, the comparison of all results obtained from il-10 assay with either
intracellular or secreted form showed that intracellular assay could be more sensitive than ElisA. specific igE was significantly
decreased after therapy in response to either Cpg/Ch.a or Cpg/rCh.a stimulant.
Conclusion. treatment of PBmCs from Ar patients with Cpg/Ch.a or Cpg/rCh.a inhibits th2 cytokine responses and induces th1biased
immune responses. Also, treatment with intranasal corticosteroids and antihistamines could enhance this Cpg effect, in vitro.
However, further clinical studies are recommended to confirm these findings.
1403
TrEaTmET oF CHroniC allErgiC rHiniTiS:inTranaSal VS. oral THEraPY?
Eatemadi, A.
immunology & infectious disease, Ahvaz univrsity of medical siences, Ahvaz, iran.
Background:Allergic rhinitis is common among iranian people.the aim of this study was compare the efficacy of intranasal vs. oral
therpy for chronic allergic rhinitis( CAr). method: A randomised controlled study was conducted in 51 patients with diagnosis of
CAr who divided to received either intranasal budesonide 256 mg ( group 1) or combination therapy wih 10 mg cetirizine plus 10
mg montelukast( group 2) daily for two months. results: At the end of treatment, group 1 had significantly better total nasal score
and subjective symptoms than group 2, but did not have any diffrences in post nasal drip. Conclusion: intranasal budesonide is mor
effective than cetirizine plus montelukst for treatment of chronic allergic rhinitis.
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