ABstrACts ABstrACts Conclusions: An unbalanced diet is a frequent feature in allergic children. this condition could be prevented and resolved by an adequate nutritional counselling. in particular, in our population the dietary intervention improved the quality of protein intake. Plasmatic non essential/essential aminoacids may be an useful marker of protein status. 2210 THE rolE oF SKin PriCK TEST in DiagnoSiS oF CroSS –allErgY Bartuzi, Z. 1 and napiórkowska, K. 2 1Department Allergology, Clinical immunology and internal Diseases, Collegium medicum nicolaus Copernicus University, Bydgoszcz, Poland. 2Allergology, Clinical immunology and internal Diseases, Collegium medicum nicolaus Copernicus University, Bydgoszcz, Poland. Background: Patients with birch pollen allergy frequently develop hypersensitive reactions to certain food e.g. apples, celery and carrots due to cross-reactivity reactivity between these allergens. these reactions result from the similarity of allergen proteins structure, which are sometimes unbound phylogenetically. the aim of this study was to investigate the role of cross-reactivity and the diagnostic value of skin prick test (using commercial and native extracts of allergens), total and specific igE in diagnosis such kind of allergy. material and methods: Fifty eight patients at the age above 16 were included in the study. the clinical history and the positive result of the skin prick test with the birch extract were the condition for qualifications. Patients were divided into two groups. Patients included in the first group were birch allergic without any symptoms after eating food (23 persons). Patients in the other group had birch pollen allergy and they had reported clinical symptoms after eating foods such as: apple, celery, carrot, tomato, banana, peach, peanut and hazelnut. (35 persons). the skin prick tests with pollen and food commercial extract and with native food allergens were determined for all individuals. results: All patients showed positive skin prick test with other pollen allergen extracts. Particularly a high percentage of positive skin reaction was find for hazel (78,3% in the first group vs. 97,1% in the second group) alder (82,6% vs. 97,1%). Patients included in the first group had positive skin reaction more often for grass and cereals (61% vs. 48,6%) and poplar (30,4% vs. 11,4%). Patients from the other group were characterized by a significantly higher percentage of positive skin tests with weeds. generally, skin prick tests with native allergens showed more positive skin reaction and a better agreement with clinical history than skin prick tests with commercial extracts. Conclusions: the skin prick tests with native allergens seems to be the method of verifying and supplementing other diagnostic tests in patients with food allergy. 2211 THE EFFECTiVEnESS oF THE mEaSUrEmEnT oF ToTal anD SPECiFiC igE in DiagnoSiS oF CroSS allErgY Bartuzi, Z. 1 and napiórkowska, K. 2 1Department Allergology, Clinical immunology and internal Diseases, Collegium medicum nicolaus Copernicus University, Bydgoszcz, Poland. 2Allergology, Clinical immunology and internal Diseases, Collegium medicum nicolaus Copernicus University, Bydgoszcz, Poland. Background: some patients with birch pollinosis frequently develop hypersensitive reactions to certain plant food (i.e. apples, celery and carrots). this phenomenon is caused by cross-reactivity between inhalant and food allergens. Exposure to inhalant allergens causes hypersensitivity to the other allergens. the aim of this study was to investigate the diagnostic value of measurement total and specific igE in diagnostic such kind of allergy. material and methods: Fifty eight patients at the age above 16 were included in the study. the clinical history and the positive result of the skin prick test with the birch extract were the condition for qualifications. Patients were divided into two groups. Patients included in the first group were birch allergic without any symptoms after eating food (23 persons). Patients in the other group had birch pollen allergy and they reported clinical symptoms after eating foods such as: apple, celery, carrot, tomato, banana, peach, peanut and hazelnut (35 persons). igE concentration (total and specific) were determined for all individuals. results: no difference in total igE levels was found between the two groups (271,5+/-403,8 iU/ml vs 242,5+/- 340,9 iU/ml). Patients with birch allergy and hypersensitivity to food allergens showed significantly higher birch pollen specific igE levels and it might be the predictive factor of developing cross allergy to distinct plant food which may develop later (11,8 +/- 14,1 iU/ml vs 4,1 +/- 6,6 iU/ml). the measurement of total and specific igE was characterized by the lowest effectiveness in diagnostic of allergy in comparison with skin tests (with commercial and native extracts of allergens). Conclusions: the measurement of total and specific igE seems to be the valuable method of verifying and supplementing other diagnostic tests in patients with food allergy. igE may be an alternative in case of questionable or negative skin prick tests and when the sPt cannot be performed. www.worldallergy.org 128 FinAl PrOgrAm
ABstrACts 2212 aSSESSing PoTEnTial DETErminanTS oF PoSiTiVE ProVoCaTion TESTS in SUBJECTS WiTH nSaiD HYPErSEnSiTiViTY Viola, m. , rumi, g. , Valluzzi, r. l. , gaeta, F. , Caruso, C. and romano, A. <strong>Allergy</strong> Unit, Complesso integrato Columbus, rome, italy. Background: Provocation tests (Pts) with the suspected compounds are considered the “gold standard” for establishing or excluding a diagnosis of hypersensitivity to nonsteroidal anti-inflammatory drugs (nsAiDs) [1]. However, only a few studies have evaluated the potential determinants of positive responses to Pts. the aims of this study are to asses the reliability of clinical histories as indicators of nsAiD hypersensitivity, as well as the risk factors for a positive Pt. methods: 275 subjects with an unequivocal history of nsAiD hypersensitivity reactions underwent Pts with the suspected drugs. to establish the potential determinants of positive Pts, we examined the following variables: gender, age at the time of reaction (12 months), and inclusion in a category of the stevenson et al. classification [2]. results: 214 (77.8%) subjects tolerated the suspected drugs and 61 (22.2%) reacted. Age
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