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Official Journal of the American College of Sports Medicine<br />

Mean Local Sweat Rate (mg/min/cm2) ± SD<br />

Drink 15 min 30 min 50 min 60 min 75 min<br />

1.5ºC 0.20 ± 0.09 0.40 ± 0.11 0.53 ± 0.12 0.58 ± 0.14 0.69 ± 0.15<br />

37ºC 0.23 ± 0.11 0.50 ± 0.17 0.61 ± 0.22 0.66 ± 0.24 0.72 ± 0.27<br />

50ºC 0.29 ± 0.15 0.61 ± 0.18 0.74 ± 0.21 0.79 ± 0.22 0.78 ± 0.23<br />

NG 50ºC 0.39 ± 0.23 0.65 ± 0.23 0.70 ± 0.23 0.71 ± 0.23 0.69 ± 0.24<br />

NG 1.5ºC 0.27 ± 0.11 0.43 ± 0.15 0.49 ± 0.16 0.51 ± 0.15 0.57 ± 0.16<br />

SW 50ºC 0.29 ± 0.17 0.50 ± 0.20 0.57 ± 0.24 0.60 ± 0.21 0.61 ± 0.21<br />

SW 1.5ºC 0.36 ± 0.29 0.51 ± 0.24 0.62 ± 0.22 0.60 ± 0.24 0.60 ± 0.24<br />

1493 Board #85 May 30, 3:30 PM - 5:00 PM<br />

sweat sodium Content: Concurrent Validity Of The horiba<br />

C-122 Tm and Wescor sweat Chek Tm analysers<br />

Eric DB Goulet, Tommy Dion, Audrey Asselin, Félix A. Savoie.<br />

University of Sherbrooke, Sherbrooke, QC, Canada.<br />

(No relationships reported)<br />

Currently, there exists two instruments that coaches and scientists can use to measure<br />

sweat sodium content under field conditions: the Horiba C-122TM and Wescor Sweat<br />

ChekTM. The former device (ion selective electrode technology) is low weight (~<br />

40g), low cost (~250 US$), pocket-sized and battery operated, whereas the latter (ion<br />

conductance technology) is semi portable (~1 kg), medium-priced (~2000 US$) and<br />

electrically operated. No studies have yet concurrently compared the validity of both<br />

instruments. This is an important issue, given the discrepant cost and physical and<br />

technical characteristics of the two devices.<br />

PurPOsE: To simultaneously determine the validity of the Horiba C-122TM and<br />

Wescor Sweat ChekTM against sweat sodium concentration values obtained with<br />

the flame photometry technique, which is the accepted reference method for the<br />

measurement of bodily sodium contents.<br />

METhOds: Sweat sodium samples (N=74) (patch collection technique, 5 x 7 cm<br />

non-adherent pads covered with highly-adhesive transparent dressings) were obtained<br />

from 26 exercising, heat-acclimatized soccer players, runners and triathletes, who<br />

each provided between 1 to 4 sweat samples from 1 to 4 different anatomical sites<br />

(forefront, thigh, upper back or forearm).<br />

rEsuLTs: Data were normally distributed (kolmogorov-smirnov test) and showed<br />

no sign of heteroscedasticity (standardized residuals vs. dependent variables). Sweat<br />

sodium concentration values obtained with the Horiba C-122TM and Wescor Sweat<br />

ChekTM correlated highly with those determined by flame photometry (Pearson<br />

product-moment correlation coefficient: r=0.98 vs. r=0.99; single measures (two-way<br />

random/absolute agreement) intra-class correlation coefficient: r=0.97 vs. r=0.98). In<br />

comparison to flame photometry, the Horiba C-122TM and Wescor Sweat ChekTM<br />

demonstrated similar biases (-3 mmol/L vs. 1 mmol/L), pure error of measurements (6<br />

mmol/L vs. 5 mmol/L), standard error of the estimates (5.51 mmol/L vs. 3.53 mmol/L)<br />

and 95% Bland and Altman level of agreement confidence intervals (-3 ± 12 mmol/L<br />

vs. 1 ± 10 mmol/L).<br />

CONCLusION: These results indicate that both the Horiba C-122TM and Wescor<br />

Sweat ChekTM portable sodium analysers demonstrate a moderate level of agreement<br />

with the flame photometry technique.<br />

1494 Board #86 May 30, 3:30 PM - 5:00 PM<br />

sweat Electrolyte Content and Fluid Loss in Elite Triathletes<br />

Riana R. Pryor, Amy B. Mausser, Douglas J. Casa, FACSM,<br />

J Luke Pryor, Lawrence E. Armstrong, FACSM, Evan C.<br />

Johnson, Brittanie M. Volk, Amy L. McKenzie, Luke N. Belval.<br />

University of Connecticut, Storrs, CT.<br />

(No relationships reported)<br />

Knowledge of 24-hour sodium and water turnover in trained triathletes during<br />

training in a warm environment is important for personalized nutrition, hydration and<br />

performance.<br />

PurPOsE: This study investigated triathlete urine and sodium 24-hour turnover<br />

during a period that included endurance exercise in a warm environment to determine<br />

if increases or decreases in consumption are necessary.<br />

METhOds: Thirty-three sponsored triathletes (19 men, 14 women; age: 37 ± 11 y,<br />

32 ± 6 y; height: 181 ± 6 cm, 170 ± 4 cm; weight: 75.7 ± 7.6 kg, 60.1 ± 5.9 kg; best<br />

half Ironman time 4:16 ± 0:24 hr:min, 4:42 ± 0:20 hr:min, respectively) completed a<br />

43.4 ± 3.8 minute treadmill run or friction resisted cycle ergometer ride (28.0 ± 0.5 ºC;<br />

27.2 ± 4.9 % RH) at a self-selected intensity which was estimated to be similar to half<br />

Ironman triathlon race pace for the chosen modality. Whole body wash-down (WBW)<br />

determined sweat electrolyte content. Twenty-four hour urine and dietary records were<br />

collected to calculate sodium and water intake and losses.<br />

rEsuLTs: Both 24-hour water and sodium balance were similar between men and<br />

women.<br />

Vol. 45 No. 5 Supplement S289<br />

Water (L·d-1 ) Sodium (mg)<br />

Male Female Male Female<br />

Total Intake 4.1 ± 1.9 3.0 ± 1.2 5081 ± 2277 3589 ± 1005*<br />

Exercise Intake 0.4 ± 0.4 0.3 ± 0.2 --- ---<br />

Sweat Output 1.4 ± 0.3 1.0 ± 0.3* 1423 ± 438 941 ± 476*<br />

Urine Output 2.8 ± 0.8 2.4 ± 0.7 4687 ± 1419 2791 ± 1162*<br />

Net Balance -0.2 ± 1.9 -0.5 ± 1.4 -1048 ± 2229 -161 ± 1435<br />

Note. *Difference between sexes, p ≤ 0.05. Total water intake includes exercise<br />

intake.<br />

CONCLusION: Twenty-four hour sodium and water turnover were similar<br />

between trained male and female triathletes. When exercising in warm conditions,<br />

trained triathletes may need to slightly increase water replacement (5-17%) and<br />

moderately increase sodium supplementation (4-21%) to remain homeostatic.<br />

Supported by Timex, Inc., Middlebury, CT.<br />

1495 Board #87 May 30, 3:30 PM - 5:00 PM<br />

Physiological Effects of Compression Garments during<br />

uncompensable heat stress<br />

Joshua T. Bautz, David Hostler, FACSM, Priya Khorana, Joe<br />

Suyama. Emergency Responder Human Performance Lab,<br />

University of Pittsburgh, Pittsburgh, PA.<br />

(No relationships reported)<br />

Thermal protective clothing (TPC) worn by firefighters protect users from flames but<br />

prevent evaporative cooling. Volume lost from sweating combined with heat stress<br />

and physical exertion places considerable strain on the cardiovascular system. Lower<br />

extremity compression garments (LECGs) have gained popularity with endurance<br />

athletes and are purported to improve performance by augmenting venous return. We<br />

hypothesized that the use of LECGs would therefore preserve intravascular volume<br />

leading to a lower heart rate (HR) while working in a hot environment.<br />

PurPOsE. Examine the effect of wearing LECGs under TPC on HR, core<br />

temperature (Tc), and markers of hydration during treadmill exercise.<br />

METhOds. Ten male subjects (mean age 27± 6 years, height 1.78± 0.092 m, mass<br />

74.75± 6.974 kg, body fat 10.6± 4.22%, VO2 max 57.8± 9.29 mL/kg/min) completed<br />

the control (no LECG) and LECG experimental conditions in randomly assigned<br />

order. Protocols were separated by a minimum of three days. Subjects exercised for<br />

90 minutes on a treadmill while wearing wilderness firefighter TPC and helmet in a<br />

heated room. Venous blood was drawn pre and post exercise to measure hemoglobin<br />

(Hgb), hematocrit (Hct), serum OSM (OSM), and serum CPK (CPK). Vital signs and<br />

perceptual measures of comfort and exertion were recorded every five minutes during<br />

the protocol. Paired samples t-tests were performed to compare the change in metrics<br />

between the two conditions.<br />

rEsuLTs. In comparing the LECG condition with the control condition, there were<br />

no differences in the change in HR (84±27 vs. 85±14 bpm, p=0.9), Hgb (0.49±0.66<br />

vs. 0.33±1.11 g/dL, p=0.7), Hct (1.22±1.92 vs. 1.11±3.62%, p=0.9), or body mass<br />

(1.72±0.78 vs. 1.77±0.58 kg, p=0.7). There were no differences in distance walked<br />

(3.9±0.5 vs. 4.0±0.5 miles, p=0.2), exercise interval (88.6±3.5 vs. 88.4±3.6 minutes,<br />

p= 0.8), or perceptual measures of comfort or exertion. Additionally, there were<br />

no differences in Tc rise (1.8±0.6 vs. 1.9±0.5°C, p=0.394), OSM (1.67±6.34 vs.<br />

6.22±11.39 mOsm/kg, p = 0.3), or CPK (22.2±30.2 vs. 29.8±19.4 IU/L, p = 0.5).<br />

Conclusions. LECG worn beneath TPC did not acutely alter the physiologic response<br />

to exertion in TPC. These data neither encourage nor discourage LECG use during<br />

uncompensable heat stress.<br />

1496 Board #88 May 30, 3:30 PM - 5:00 PM<br />

Effects of different Types of Clothing on Thermoregulation<br />

and Microenvironments during simulated american<br />

Football<br />

Eric J. Jones, Nathan Frischmann, Mark D. Faries, Wesley<br />

Kephart. Stephen F. Austin State University, Nacogdoches, TX.<br />

(Sponsor: Thomas J. Pujol, FACSM)<br />

(No relationships reported)<br />

PurPOsE: This study assessed the effects of two different types of clothing on core<br />

temperature (Tc), skin temperature (Ts), and the microenvironments (ME) of collegeaged<br />

males during a simulated football game within hot environments.<br />

METhOds: Trained college-aged males (n = 10) participated in a series of 5-second<br />

ergometer sprints in a climate-controlled chamber (35°C, 55%RH). The sprint protocol<br />

was based on the average number and length of plays within NCCA Division I<br />

football games. Subjects performed two separate trials, each consisting of 85 minutes,<br />

separated by a 48-hour washout period. During trials, either cotton or channeled fabric<br />

clothing (CFC) was donned under protective football gear. Tc, Ts, and ME temperature<br />

and humidity was monitored throughout trials.<br />

rEsuLTs: Paired samples t-test revealed significant differences (p

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