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Official Journal of the American College of Sports Medicine 1358 Board #6 May 30, 1:00 PM - 3:00 PM Effects Of a Freeze-dried Fruit-vegetable Juice Powder On Exercise-induced Metabolic Outcomes: a Metabolomics approach Amy M. Knab 1 , David C. Nieman, FACSM 1 , Nicholas D. Gillitt 2 , R. Andrew Shanely 1 , Lynn Cialdella-Kam 1 , Dru A. Henson 3 , Wei Sha 4 , Mary Pat Meaney 1 . 1 Appalachian State University, Human Performance Laboratory, North Carolina Research Campus, Kannapolis, NC. 2 Dole Nutrition Research Laboratory, North Carolina Research Campus, Kannapolis, NC. 3 Appalachian State University, Boone, NC. 4 Bioinformatics Services Division, University of North Carolina at Charlotte, North Carolina Research Campus, Kannapolis, NC. Supported by a.M. Knab: Contracted Research - Including Principle Investigator; Dole Foods Inc.. PurPOsE: To investigate a freeze-dried fruit-vegetable juice powder (JUICE) in decreasing exercise-induced inflammation, oxidative stress, and immune perturbations. METhOds: Thirty-four endurance cyclists (25 male, 9 female) were randomized to control (non-JUICE) or JUICE for 17 days. JUICE provided 230 mg of flavonoids/ day, doubling the typical adult daily intake. On days 15-17, subjects cycled at 70-75% VO2max for 2.25-h, followed by a 15 min time trial. Blood samples were collected pre-suppl, post-suppl (pre-exercise), and immediately post-exercise and 14-h postexercise on the third day. Samples were analyzed for inflammation [interleukin (IL)- 6, IL-8, tumor necrosis factor alpha (TNFα), monocyte chemoattractant protein 1 (MCP-1)], oxidative stress (ORAC, FRAP, reduced and oxidized glutathione, protein carbonyls), and immune function [granulocyte (G-PHAG) and monocyte (M-PHAG) phagocytosis and oxidative burst activity] biomarkers, with metabolomics conducted using gas and liquid chromatographic mass spectrometry (GC-MS; LC-MS). rEsuLTs: A 2 (group) x 4 (time points) repeated measures ANOVA revealed significant time effects due to 3 days of exercise for IL-6 (396% increase), IL-8 (78% increase), TNFα (12% increase), MCP-1 (30% increase), G-PHAG (38% increase), M-PHAG (36% increase), FRAP (12.6% increase), ORAC (11% decrease at 14-h post-exercise), and protein carbonyls (82% increase at 14-h post-exercise) (p < 0.01). No significant interaction effects were found for all of the inflammation, oxidative stress, and immune function measures. Repeated measures ANOVA revealed a >2-fold shift in 103 metabolites due to exercise in the non-JUICE condition (FDR p-values all 20% DV) of five nutrients (22% vitamin A, 245% vitamin C, 20.5% vitamin B6, 23.6% potassium, and 33.7% folate). Funded by Dole Foods Inc. D-15 Thematic Poster - Immunology May 30, 2013, 1:00 PM - 3:00 PM Room: 209 1359 Chair: Jeffrey A. Woods, FACSM. University of Illinois, Urbana, IL. (No relationships reported) 1360 Board #1 May 30, 1:00 PM - 3:00 PM Microarray analysis revealed Gene Expression Profiling In Lps- stimulated Whole Blood Following Prolonged Exhaustive Exercise Asghar Abbasi1 , Melanie Hauth1 , Michael Walter2 , Jens Hudemann3 , Veit Wank4 , Andreas M. Niess3 , Hinnak Northoff1 . 1Clinical and Experimental Transfusion Medicine, Tübingen, Germany. 2Medical Genetics, Tübingen, Germany. 3Institute of Sports Medicine, Tübingen, Germany. 4Institute of Sports Science, Tübingen, Germany. (No relationships reported) An acute bout of prolonged exhaustive exercise can cause adverse effects on the immunity reflected by transient immunosuppression following the event. PurPOsE: To gain more insight into these mechanisms, the capacity of whole blood cultures in profiling gene expression in response to endotoxin (LPS) was studied in athletes before, 30min after, 3h after and 24h after a half-marathon run. METhOds: Eight well trained men and 8 well trained women were participated Vol. 45 No. 5 Supplement S257 and gene expression patterns were assessed in LPS-stimulated (1h) and un-stimulated whole blood using Affymetrix GeneChip microarrays. rEsuLTs: Results showed that prolonged exhaustive exercise altered several genes in LPS-stimulated cultures relative to un-stimulated cultures. These included genes related to inflammatory response (30min and 3h post-exercise/both sexes), activation of AMPK activity, response to hypoxia (3h post exercise/men), angiogenesis (3h post exercise/women), prostaglandin biosynthetic process (30min and 3h post-exercise/ both sexes), myeloid cell differentiation (24h post exercise/women) Functional categorization (KEGG pathway analysis) showed that genes associated with TLRs signalling were the most significantly over presented in both sexes. CONCLusION: The results of the present study indicate that prolonged exhaustive exercise resulted in a rich variety of changes in expression of inflammatory and antiinflammatory genes in short-time LPS stimulated whole blood cultures. This may provides novel insights into the molecular processes involved in the immune response following exhaustive exercise. This work was supported by a grant from the Bundesinstitut für Sportwissenschaften (Bonn, Germany, KZIIA1-070108/08-09). 1361 Board #2 May 30, 1:00 PM - 3:00 PM The Impact of Ironman Triathlon on Innate Immune Cell Numbers and Function Shlomit Radom-Aizik 1 , Carl M. Maresh, FACSM 2 , Fadia Haddad 1 , Dan M. Cooper 1 , Cherryl Nugas 1 , Douglas J. Casa, FACSM 2 , Jenna M. Apicella 2 , Elaine C. Lee 2 , Julie K. DeMartini 2 , Colleen X. Munoz 2 , Frank Zaldivar 1 . 1 Pediatric Exercise Research Center, UC Irvine, Irvine, CA. 2 Korey Stringer Institute, University of Connecticut, Storrs, CT. (Sponsor: Carl M. Maresh, FACSM) (No relationships reported) Prolonged, high-intensity endurance exercise activities such as the Ironman Triathlon (typically lasting 10-12 hours) profoundly perturb homeostasis in a variety of physiological systems, and would likely impact key cellular components of innate immunity (e.g., natural killer cells and monocytes), a “first responder” to stress and infection. The effect of such exercise on numbers and functional indexes of circulating innate immune cells is poorly understood. PurPOsE: To study the impact of the Ironman Triathlon on innate immune cell number and function. We hypothesized that there would be immediate effects on measures of number of cells and cellular activation in NK cells (KIR, killer cell immunoglobulin-like receptors) and in monocytes (toll-like receptors, TLR) which would continue throughout the recovery period. METhOds: 10 elite male triathletes, 32-64 y/o volunteered. Blood was drawn between 2-3 days before the triathlon (baseline), and during recovery at 1-, 24-, and 48-h after completion. CBC and standard flow cytometry methods were used to determine monocyte and NK cell (CD3 - CD56+) count, NK dim and bright subpopulation (CD56 dim and CD56 bright ), and cell function (NK, KIR2DL2; and monocyte, TLR4). Repeated Measure ANOVA was applied (p
<strong>Thursday</strong>, May 30, 2013 S258 Vol. 45 No. 5 Supplement rEsuLTs: Elite marathon runners tend to have lower concentrations of salivary antimicrobial peptides (HBD-2 and LL-37) than sedentary subjects(P
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