Annual Progress Report on Malting Barley Research March, 2007

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towards the development of RWA and Greenbug resistant winter hulless barleys adapted to Oklahoma with the end use of ethanol production. Barley accessions recently added to the NSGC were screened for RWA1 resistance. Other barley research and future direction of the program A cooperative project with Lynn Dahleen, USDA-ARS, Fargo, ND, entered its second year to find genetic markers for RWA resistance in STARS 9301B and STARS 9577B to aid in genetic diversity studies and in marker assisted selection of resistant germplasm lines. 300 F2:F3 lines from the STARS 9577B population were phenotyped twice for this project in 2006. A project has been initiated to develop hulless winter barleys for ethanol production in the Southern Plains. 80 winter hulless barley accessions and 240 hulled winter barley lines were grown replicated tests and evaluated at 2 locations in Oklahoma in 2006. The hulled barleys were in a Post 90 background with RWA resistance from 10 different sources. Post 90 is also resistant to all currently identified biotypes of Greenbug. Barley grown in Oklahoma will need resistance to both RWA and Greenbug. All hulless lines were evaluated for %starch by Patricia Rayas-Duarte at Oklahoma State University. 19 hulless lines were selected for crossing with 25 RWA-resistant, hulled germplasm lines. Project Personnel Germplasm Enhancement Dolores W. Mornhinweg, Geneticist David R. Porter, Research Geneticist Gary Puterka, Research Entomologist Cooperators Don Obert, Research Agronomist, USDA-ARS, Aberdeen, ID Phil Bregitzer, Research Geneticist, USDA-ARS, Aberdeen, ID Frank Peairs, Professor, Colorado State University An, Hang, Research Geneticist, USDA-ARS, Aberdeen, ID Dave Blatensperger, Professor, University of Nebraska Lynn Dahleen, USDA-ARS, Fargo, ND Tim Springer, Research Geneticist, USDA-ARS, Woodward, OK Brett Carver, Professor, Oklahoma State University Wynse Brooks, Professor, Virginia Polytechnic Institute and State University 100
Kevin Hicks, Research Leader, USDA-ARS, Wyndmoor, PA Patricia Rayas-Duarte, Professor, Oklahoma State University Recent Publications Bregitzer, P., D.W. Mornhinweg, and B.L. Jones. 2003. Resistance to Russian wheat aphid damage derived from STARS-9301B protects agronomic performance and malting quality when transferred to adapted barley germplasm. Crop Sci. 43(6): 2050-2057. Bregitzer, P., D.W. Mornhinweg, R. Hammon, M. Stack, D.D. Baltensperger, G.L. Hein, M.K. o’Neill, J.C. Whitmore and D.J. Fiedler. 2005. Registration of ‘Burton’ Barley. Crop Sci. 45: 1166. Mornhinweg, D.W., D.R. Porter and J.A. Webster. 1999. Registration of Stars-9577B Russian Wheat Aphid Resistant Barley Germplasm. Crop Sci. 39: 882-883. Mornhinweg, D.W. 2003. Invasion of Barley Insects – Yield and Quality Snatchers: We dare not rest. Proceedings 34 th Barley Improvement Conference. Morhninweg, D.W., L.H. Edwards, E.L. Smith, G.A. Morgan, D.R. Porter, and B.F. Carver. 2004. Registration of Post 90 Barley. Crop Sci. 44:2263. Mornhinweg, D.W.,D.E. Obert, D.M. Wesenberg, C.A. Erickson, and D.R. Porter. 2006. Registration of seven winter feed barley germplasms resistant to Russian wheat aphid. Crop Sci. 46: 1826-1827. Mornhinweg, D.W., M.J. Brewer and D.R. Porter. 2006. Effect of RWA on yield and yield components of field grown susceptible and resistant spring barley. Crop Sci. 46:36-42. Porter, D.R., D.W. Mornhinweg, and J.A. Webster. 2004. New Sources of Resistance to Greenbug in Barley. Crop Sci. 44: 1245-1247. Porter, D.R. and D.W. Mornhinweg. 2004. Characterization of greenbug resistance in barley. Plant Breeding 123:5 pp. 493-494. 101
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
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EARLY GENERATION LINES Forty F5 pop
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have evaluated a PCR based marker,
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Edward Schiefelbein, Research Scien
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small grains pathology input to thi
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Project Personnel Small Grains Path
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Investigations on Barley Diseases a
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Minnesota barley disease survey and
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pathotypes at the seedling stage in
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germplasm with the highest level of
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Mirlohi, A., Brueggeman, R., Steffe
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Developing Improved Malt Barley Var
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Table 2. Montana’s Statewide Tria
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As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74: 6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76: Objectives Met in the One-Year Fund
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Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85 and 86: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
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Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125: Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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