Annual Progress Report on Malting Barley Research March, 2007

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MALTING AND BREWING QUALITY OF BARLEY Dr Paul Schwarz Department of Plant Sciences North Dakota State University Fargo, ND 58105 EXECUTIVE SUMMARY The primary goals of the Barley Quality Program at NDSU are to provide individuals in the barley improvement program with barley and malt quality analytical services, 2.) to conduct applied research that addresses issues of immediate concern and improves the understanding of malting quality, 3.) to conduct the annual survey of regional barley crop quality, and 4.) to provide training in cereal chemistry with particular emphasis on the science of malting and brewing. Major Objectives and Expected Benefits (goals 1 and 2: AMBA funded projects): Barley and Malt Quality Analyses: Malt quality analyses provided to individuals in the NDSU barley variety development program (and others) directly assists AMBA in meeting its mission of providing the malting and brewing industries with an abundant supply of high quality malting barley. Early generation screening for malt quality is important in streamlining the overall malting barley variety development process. Elimination of undesirable materials at an early stage increases the overall quality of materials submitted for laboratory malting. Barley and malt quality analyses conducted for breeding research projects contribute to a more complete understanding of the genetics of malting quality. Likewise, quality analyses conducted for barley production studies help maintain barley as a competitive crop within the region. Research Studies - Applied quality research directly addresses the AMBA mission to increase the understanding of malting barley. Projects which have addressed immediate issues have had major impact. Our past research work on pre-harvest sprouting and deoxynivalenol are important examples of immediate issues. 74
Objectives Met in the One-Year Funding period Barley and Malt Quality Analyses • Screened 3350 early generation samples for barley quality, and 130 variety plot samples for malt quality. o Data for the NDSU Variety Development Program was used to make decisions on lines submitted for advanced testing. • Micro-malted and provided malt quality analyses (n=181) for a study on transgenic barley showing partial resistance to FHB. • Micro-malted and provided malt quality analyses (n=98) for a study on adaptation of European germplasm in western ND and eastern MT. o These lines may prove of value under irrigation conditions, and may provide growers with a competitive crop (barley) until adapted domestic cultivars are released. Research • Identified significant interference from beta-amylase in the standard ASBC method for the determination of alpha-amylase in malt (method Malt-7) Most Significant Accomplishment • Provided value services to barley grower, industry and researcher stakeholders, while maintaining a good balance of applied barley research. 75
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
Page 23 and 24: EARLY GENERATION LINES Forty F5 pop
Page 25 and 26: have evaluated a PCR based marker,
Page 27 and 28: Edward Schiefelbein, Research Scien
Page 29 and 30: small grains pathology input to thi
Page 31 and 32: Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73: 6. Lee, S. and Neate, S.M. (2006) P
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85 and 86: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102: Kevin Hicks, Research Leader, USDA-
Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125:
Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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