Annual Progress Report on Malting Barley Research March, 2007

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quality, on the other hand, remain largely unchanged since the 1940’s, and do not necessarily address the needs of brewers. Research directed at re-evaluation of some test methods, and their foundations may be particularly pertinent. We are proposing to continue with evaluation of factors that control laboratory malt extract, and their relationship to extract factors that are not routinely determined, such as fermentability. A chemometrics approach will be utilized. Nevertheless, Fusarium Head Blight remains the most critical problem facing barley producers and the malting and brewing industries. We will continue to work in conjunction with breeders and pathologists on the development of FHB resistant cultivars. LABORATORY PERSONNEL Dr Paul Schwarz Professor Dr Yin Li Post-Doctoral Scientist Dr Dennis Tobias Post-Doctoral Scientist Mr. John Barr Chemist (Malt Quality) Ms Karen Hertsgaard IBMS Barley Information Specialist Mr. James Gillespie Chemist (DON) Ms Debra Hatzenbeller Chemist (DON) Mr Javier Delgado Graduate Research Assistant (Plant Pathology) Ms Xinrong Dong Graduate Research Assistant Ms Zhou Bing Graduate Research Assistant Ms Jun Ying Yan Laboratory Assistant (DON) Mr. Evan Cummings Laboratory Assistant (DON) COOPERATORS Dr Phil Bregitzer USDA-ARS, Aberdeen, ID Dr Lynn Dahleen USDA-ARS NCSL, Fargo, ND Dr Leif-Alexander Gabe Technical University Berlin, Germany Dr Neil Gudmestad Dept. of Plant Pathology, NDSU Dr Guo-Qing He Zhejiang University, PRC Dr Charlene Wolf-Hall Dept. of Veterinary Sciences, NDSU Dr Nick Hill Dept. of Crop and Soil Sci., University of Georgia Dr Richard Horsley Dept. of Plant Sciences, NDSU Dr Stephen Meinhardt Dept. of Plant Pathology, NDSU Dr Stephen Neate Dept. of Plant Pathology, NDSU Dr Gary Secor Dept. of Plant Pathology, NDSU 90
PUBLICATIONS (2006-07) Schwarz, P.B., Li, Y., Barr, J.M., and Horlsey, R.D. Effect of operational parameters on the determination of laboratory extract and associated wort quality factors. Accepted: J. Am. Soc. Brew. Chem. February 2007. Li, Y., Xu, Y., Schwarz. P.B., and Gu, G. Organic acids of commercial beers in China: A chemometric study. Accepted: J. Am. Soc. Brew. Chem. September 2006. Manoharana, M., Dahleen, L.S., Hohnc, T.M., Neate, S.M., Yue, X.-H., Alexander, N.J., McCormick, S.P. Bregitzer, P., Schwarz, P.B., and Horsley, R.D. Expression of 3-OH trichothecene acetyltransferase in barley (Hordeum vulgare L.) and effects on deoxynivalenol. Plant Science (6): 699-706, 2006. Schwarz, P.B., Hill, N., and Rottinghaus, G.E. Fate of ergot (Claviceps purpurea) alkaloids during malting and brewing. J. Am. Soc. Brew. Chem. 65(1):1-8, 2007. Hill, N.S., Schwarz, P.B., Dahleen, L. S. Neate, S.M., Horsley, R., Glenn, A.E. and O'Donnell , K. ELISA analysis for Fusarium in barley: development of methodology and field assessment. Crop Sci. 46: 2636-2642, 2006. Schwarz, P.B., Neate, S.M., and Rottinghaus, G.E. Widespread occurrence of ergot (Claviceps pupurea) in upper Midwestern U.S. barley. Plant Disease 90:527, 2006. Horsley, R. D., Franckowiak, J.D. P.B. Schwarz, P.B. and S.M. Neate, S.M. Registration of ‘Stellar-ND’ Barley. Crop Sci 46:980-981, 2006 Horsley, R.D. Schmierer, D. Maier, C., Kudrna, D. Urrea, C., Steffenson, B.J., Schwarz, P.B., Franckowiak, J.D., Green, M., Zhang, B., Kleinhofs, A. Identification of QTLs associated with Fusarium Head Blight resistance in barley accession CIho 4196. Crop Sci. 46: 145-156, 2006. He, G.-Q., Wang, Z.-Y., Liu, Z.-S., Chen, Q.H., Ruan, H., and Schwarz, P.B. Relationship of proteinase activity, foam proteins and head retention in unpasteurized beer. J. Am. Soc. Brew. Chem. 64(1):33-38, 2006. Schwarz, P.B., Horsley, R.D., Steffenson, B.J., Salas, B., and Barr, J.M. Quality risks associated with the utilization of Fusarium Head Blight infected malting barley. J. Am. Soc. Brew. Chem. 64(1):1-7, 2006 91
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
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EARLY GENERATION LINES Forty F5 pop
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have evaluated a PCR based marker,
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Edward Schiefelbein, Research Scien
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small grains pathology input to thi
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Project Personnel Small Grains Path
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Investigations on Barley Diseases a
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Minnesota barley disease survey and
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pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74: 6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76: Objectives Met in the One-Year Fund
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85 and 86: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89: forms (through enzymolysis during m
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102: Kevin Hicks, Research Leader, USDA-
Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125: Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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