Annual Progress Report on Malting Barley Research March, 2007

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FHB, Spot Blotch, Net Blotch and CRR Germplasm screening Dr Phil Bregitzer, USDA-ARS, Aberdeen ID Dr. Flavio Capettini, ICARDA/CIMMYT, Mexico. Dr. Lynn Dahleen, USDA-ARS, Fargo, ND Mr Scott Halley Langdon Research Extension Centre, ND Prof. Richard Horsley, NDSU, Fargo, ND Prof Brian Steffenson, University of Minnesota, MN Leaf Rust Genetics Dr. T Friesen, USDA-ARS, Fargo, ND Prof. Shaobin Zhong, University of Hawaii, Honolulu, HI Septoria Genetics Dr. Stephen Goodwin, UDSA-ARS, Purdue University, IN Prof Bruce McDonald, Federal Institute of Technology, Zurich, Switzerland FHB Disease Management Prof Nick Hill, University of Georgia, GA Mr. Mark Halvorsen, North Central Research and Extension Centre, Minot ND FHB Barley Disease Modeling Prof Jeff Stein, Small Grains Pathology, South Dakota State University Publications: 1. Hill, N.S., Neate, S.M., Cooper, B., Horsley, R.D., Schwarz, P.B., Dahleen, L.S., Smith, K.P. and Dill-Macky, R. (2006) ELISA Analysis for Fusarium in Barley: Application in Field Nurseries. ASA-CSSA-SSSA International <strong>Annual</strong> Meetings, Indianapolis, November 12-16, 2006. 2. Hill, N.S., Schwarz, P., Dahleen, L.S., Neate, S.M., Horsley, R., Glenn, A.E., O’Donnell, K. (2006) ELISA Analysis for Fusarium in Barley: Development of Methodology and Field Assessment. Crop Science 2006 46: 2636-2642. 3. Horsley, R.D, Franckowiak, J.D., Schwarz, P.B. and Neate, S.M. (2006) Registration of 'Stellar-ND' Barley. Crop Science 46:980-981. 4. Lee, S. and Neate, S.M. (2006) Distribution and frequency of mating types in Septoria passerinii in North Dakota Barley. p135. Proceedings 3 rd International Workshop on Barley Leaf Blights, Edmonton Canada July 2006. Agriculture and Agri-Food Canada, Lacombe, Alberta. 5. Lee, S. and Neate, S.M. (2006) Molecular mapping of Rsp1 and Rsp3 resistance genes against Septoria passerinii in barley using DArT technology. Phytopathology 96:S66. 72
6. Lee, S. and Neate, S.M. (2006) Population genetic structure of Septoria paserinii in North Dakota Barley. p136. Proceedings 3 rd International Workshop on Barley Leaf Blights, Edmonton Canada July 2006. Agriculture and Agri-Food Canada, Lacombe, Alberta. 7. Lee, S-H. and Neate, S.M. (2006) Molecular Mapping of Rsp1, Rsp2, and Rsp3 Genes Conferring Resistance to Septoria Speckled Leaf Blotch in Barley. Phytopathology 97:155- 161. 8. Lee, S-H. and Neate, S.M. (2006) Population genetic structure of Septoria passerinii in North Dakota barley. Phytopathology (Accepted October 24 th 2006) 9. Lee, S-H. and Neate, S.M. (2006) Sequence tagged site (STS) markers to Rsp1, Rsp2, and Rsp3 genes for resistance to septoria speckled leaf blotch in barley. Phytopathology 97:162- 169. 10. Manoharan, M., Dahleen, L.S., Hohn, T., Neate, S.M., Yu, , X.-H., Alexander, N.J., McCormick, S., Schwarz, P., and Horsley, R. (2006) Expression of 3-OH trichothecene acetyltransferase in barley (Hordeum vulgare L.) and effects on Fusarium head blight. Plant Science 161:699-706. 11. Schwarz, P.B., Neate, S.M. and Rottinghaus, G.E. (2006) Widespread Occurrence of Ergot in Upper Midwestern U.S. Barley, 2005. Plant Disease 90:527. 12. Sun, Y., Zhong, S., Steffenson, B.J., Friesen, T.L. and Neate S.M., Relationship between amplified fragment length polymorphism (AFLP) and virulence variation in Puccinia hordei Can. J. Plant Pathol. (accepted September 2006) 73
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
Page 21 and 22: Lines M122 and M124 were rated sati
Page 23 and 24: EARLY GENERATION LINES Forty F5 pop
Page 25 and 26: have evaluated a PCR based marker,
Page 27 and 28: Edward Schiefelbein, Research Scien
Page 29 and 30: small grains pathology input to thi
Page 31 and 32: Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71: 2. United States Wheat and Barley S
Page 75 and 76: Objectives Met in the One-Year Fund
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85 and 86: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102: Kevin Hicks, Research Leader, USDA-
Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
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Establishing that sequence variatio
Page 125:
Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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