Annual Progress Report on Malting Barley Research March, 2007

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OTHER BARLEY SERVICE AND RESEARCH WORK (supported outside AMBA funding) 1.) Quality Testing Services (A) Regional Barley Crop Quality. The regional survey of North Dakota and Minnesota barley has been conducted by this laboratory for over 25 years, and represents an excellent example of industry-grower cooperation. The survey provides timely information on the quality of regional barley as harvest progresses, and has been an important means of documenting crop quality issues such as pre-harvest sprouting and DON. The NDBC utilizes the survey in marketing efforts. Samples and data from the crop survey have been widely utilized in research and economic studies. With all impacts considered, the annual crop survey is an important tool in maintaining barley as a competitive crop within the region. The 2006 survey of the regional barley crop was prepared in cooperation with the North Dakota Barley Council and the USDA North Dakota Agricultural Statistics Service. AMBA. A total of 233 six-rowed and 35 two-rowed samples were collected throughout North Dakota and the primary barley producing regions of Minnesota. Samples (n=9) were also collected in eastern Montana. Data was provided to AMBA. A comparative summary of data is presented in Table I Table I. North Dakota and Minnesota Six-rowed Barley Crop Quality, 2003–2006 Kernel Plumpness (%) Test Weight (lbs/bu) Protein (%) Kernel Color 2006 58.7 45.9 12.8 3 2005 75.7 46.0 12.9 5 2004 80.4 47.5 12.6 7 2003 76.3 47.8 12.8 6 Samples (n=282) were tested for DON. The average level of DON observed in the 2006 North Dakota and Minnesota Regional six-rowed barley crop was
10 9 8 7 6 5 4 3 2 1 0 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 Figure 8. Average DON levels present in the North Dakota and Minnesota Barley Crop, 1993-2005. (B) Deoxynivalenol Testing Program. The overall goal of DON testing in our laboratory is to expedite the development of scab resistant malting barley cultivars for the upper Midwest. This portion of the program is largely funded through USDA and ND state appropriated funds. DON analysis services are provided to barley breeders, geneticists, and pathologists at NDSU, University of Minnesota, Virginia Polytech., and Busch Agricultural Resources, Inc. Allotments are provided to all cooperators at no charge. Samples beyond individual allotments are analyzed at a minimal charge, which includes only the cost of expendable supplies, chemicals, and sample grinding labor. Approximately 15,000 barley samples from the 2006 crop were analyzed (or are in process) for DON by GC-ECD (Table II). This number compares to 10,000 in 2005. Samples included breeder’s lines, crop survey samples, and samples from research studies. The 2005 crop samples were analyzed beginning August 2006 and will be complete in April 2006. A barley DON check sample service is operated as a service to 15 academic and industry labs. Many of these are AMBA member companies, or are affiliated with member companies. Two samples are shipped to each participant on a monthly basis. 85
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
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EARLY GENERATION LINES Forty F5 pop
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have evaluated a PCR based marker,
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Edward Schiefelbein, Research Scien
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small grains pathology input to thi
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Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74: 6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76: Objectives Met in the One-Year Fund
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83: Figure 7. Time course of I2-binding
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102: Kevin Hicks, Research Leader, USDA-
Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125: Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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