Annual Progress Report on Malting Barley Research March, 2007

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Project Personnel: Don Obert, Dave Burrup, Chris Evans, Karla Reynolds, Kathy Satterfield Recent Publications: (2005-06): Obert, DE, DM Wesenberg, DE Burrup, BL Jones, and CA Erickson. 2006. Registration of ‘Charles’ Barley. Crop Sci. 46:468-469. Obert, DE, DM Wesenberg, DE Burrup, CA Erickson, JC Whitmore, and BL Jones. 2006. Registration of ‘Sublette’ Barley. Crop Sci. 46:989-991. J. Michael Bonman, Harold E. Bockleman, Blair J. Goates, Don E. Obert, Patrick E. McGuire, Calvin O. Qualset, and Robert J. Hijmans. 2006. Geographic distribution of common and dwarf bunt resistance in landraces of Triticum aestivum subsp. aestivum. Crop Sci. 46:1622-1629. CA Erickson, D Obert, DE Burrup, JC Whitmore, and DM Wesenberg. 2006. Registration of ‘Creel’ Barley. Crop Sci. 46:1812-1813. DW Mornhinweg, DE Obert, DM Wesenberg, CA Erickson, and DR Porter. 2006. Registration of seven winter feed barley germplasm lines resistant to Russian wheat aphid. Crop Sci 46:1826-1827. Hoffman, DL, J Chong, EW Jackson, and DE Obert. 2006. Characterization and mapping of a crown rust gene complex (Pc58) in TAM O-301. Crop Sci. 46:2630-2635. EW Jackson, DE Obert, M Menz, G Hu, JB Avant, J Chong, and JM Bonman. Characterization and Mapping Oat Crown Rust Resistance Using Three Assessment Methods. Phytopathology (Accepted). P Bregitzer, V Raboy, DE. Obert, J Windes, and JC Whitmore. Registration of ‘Herald’ barley. Crop Sci. (Accepted). An Hang, Don Obert, Ann Inez N. Gironella and Charlotte S. Burton. Evaluation of Amylose Starch and β-glucan in Barley: Their Relationships to Protein Content, Agronomic traits, and Environmental Factors. Crop Sci. (Accepted). P Bregitzer and DE Obert. Registration of 95SR316A barley germplasm. 2006. Crop Sci: Submitted. 16
Executive Summary AMBA <strong>Progress</strong> <strong>Report</strong> – 3/09/07 MINNESOTA BARLEY IMPROVEMENT PROJECT Kevin P. Smith Department of Agronomy and Plant Genetics University of Minnesota, St. Paul, MN 55108, U.S.A. The overall aim of this research is to develop new six-rowed barley varieties with acceptable malt quality, improved disease resistance, and high yield potential. This research will directly assist in AMBA’s mission to provide an adequate supply of high quality malting barley. Barley improvement at the University of Minnesota is a cooperative effort of the Department of Agronomy and Plant Genetics, the Department of Plant Pathology, and the Research and Outreach Centers of the University of Minnesota. Specific breeding goals include high yield, enhanced lodging resistance, resistance to Fusarium head blight (FHB), net blotch, spot blotch, stem rust, and Septoria speckled leaf blotch (SSLB), and favorable malting and brewing characteristics. To meet these objectives, we are conducting a comprehensive breeding and genetics research effort funded by state and federal grants. This AMBA project supports breeding activities (making crosses, population development, trait evaluation, breeding line selection) directed toward the development of new varieties. This past year one breeding line, M109, was entered into AMBA plant-scale brewing evaluation with the 2006 crop. Breeding line M115 was rated satisfactory for its second year in AMBA pilot-scale malting and will advance to plant-scale in 2008. Our first breeding line with enhanced FHB resistance was rated satisfactory in AMBA pilot malt testing (2005 crop) and entered into a second year of testing with the 2006 crop. During the previous year, we made crosses, among elite parents, designed to combine desirable traits. We developed populations through single seed decent and successfully conducted our standard set of yield and disease trials in Minnesota to evaluate breeding lines. Growing conditions were generally dry during most of the season, however, we obtained good quality data from all of our planned trials and experiments at five locations in Minnesota. Specific expected outputs on a yearly basis are: 1) development of breeding populations segregating for useful genes; 2) barley germplasm with specific desirable traits; 3) a steady flow of variety candidates into the AMBA pilot malting and plant-scale brewing programs. The following are some of the most significant accomplishments from the previous year: • Variety candidate M109 was grown for AMBA plant scale brewing evaluation with the 2006 crop. Sufficient production was obtained from 3 locations in Minnesota and North Dakota to permit plant-scale brewing. • Three of the four variety candidates (M115, M122, M124) we submitted to AMBA pilot malting evaluation were rated satisfactory with the 2005 crop. This is the second year M115 was rated satisfactory. We should have sufficient seed of M115 in 2008 to grow 17
Page 1 and 2: Annual Pro
Page 3 and 4: -i- INTRODUCTION The March 2007 <st
Page 5 and 6: USDA-ARS NORTHERN CROP SCIENCE LABO
Page 7 and 8: Spring Regional over two years at D
Page 9 and 10: Table 1. 2006 UCD Two-Rowed Malting
Page 11 and 12: environments was practiced, 2) elit
Page 13 and 14: Table 4. Selected six-rowed lines c
Page 15: Other Barley Research: Feed Program
Page 19 and 20: Table 1. Agronomic performance of M
Page 21 and 22: Lines M122 and M124 were rated sati
Page 23 and 24: EARLY GENERATION LINES Forty F5 pop
Page 25 and 26: have evaluated a PCR based marker,
Page 27 and 28: Edward Schiefelbein, Research Scien
Page 29 and 30: small grains pathology input to thi
Page 31 and 32: Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
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5. Collect isolates of barley patho
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oot rot. In collaboration with Scot
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2. United States Wheat and Barley S
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6. Lee, S. and Neate, S.M. (2006) P
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Objectives Met in the One-Year Fund
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These automated methods have been t
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Beta-limit dextrin Alpha-Amylase Al
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M w kDa 800 600 400 200 0 0.4 0 2 4
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Figure 7. Time course of I2-binding
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10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
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2. Other Research (A) Predicting Se
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forms (through enzymolysis during m
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PUBLICATIONS (2006-07) Schwarz, P.B
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CI9214, PI552963 (Heartland), and N
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showing good levels of resistance t
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Germplasm Enhancement for RWA Resis
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Gary Hein in Colorado and Nebraska.
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Kevin Hicks, Research Leader, USDA-
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Detailed Report on
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Other Barley Research and Future Di
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Table 1. 2006/2007 Oregon winter ba
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Table 4. Agronomic data for OSU win
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MALTING QUALITY ANALYSIS OF NEW BAR
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We recently secured an industrial c
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2006-2007 Progress
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enzyme it encodes. We found that se
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horseradish peroxidase-conjugated g
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found in plant cell walls. The degr
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Establishing that sequence variatio
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Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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