Annual Progress Report on Malting Barley Research March, 2007

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Table II 2006 Crop Samples Analyzed for DON in the Department of Plant Sciences Sample Type Sample Number Barley Breeding 4,668 (BARI-Cooper, Dr Griffey, Dr. Horsley, Dr Smith) 2006 Regional Crop Survey 282 Barley Pathology (Dr Neate) 4652 Barley Genetics (Dr Dahleen) 899 Research (Dr Schwarz) 2993 AMBA 20 Standards 1282 Other 503 Tot 15,299 (C) IBMS Grower Survey. A barley production and information survey was sent to 5000 barley producers in Idaho, Montana, and North Dakota by the Institute of Barley and Malt Sciences (IBMS) in late November 2006. The questionnaire was produced through the cooperative efforts of the IBMS, AMBA, the Idaho Barley Commission, the North Dakota Barley Council, the Montana Wheat and Barley Committee, the USDA/CSREES funded barley Coordinated Agricultural Project (CAP) The survey was mailed by the ND Ag Statistics Service, who also conducted telephone follow-up. Questions on the survey include such topics as yield, acreage, transportation, factors influencing barley growers decisions to produce barley, information sources, farming practices, producer support system effectiveness and satisfaction levels for growers. Approximately 1200 surveys had been received by December 15, 2006. Results are currently being analyzed. Survey incentive prizes were donated by AMBA, Anheuser- Busch, Cargill Malt, International Malting Co, Molson-Coors Brewing, and Rahr Malting Co. 86
2. Other Research (A) Predicting Seed Storage Viability with Accelerated Aging: Widespread occurrence of pre-harvest sprouting in 2002 raised concern over the storage viability of sprouted grain. We found that while severely sprouted samples rapidly lost viability in storage, common measures of sprouting were not good overall predictors (r≤0.50) of germination following storage for 4 and 7 months (Schwarz et al. J. ASBC 62(4):147, 2004). Germination following accelerated seed aging was found to be a more reliable predictor (r=0.73) of germination following 7 months of storage. Accelerated aging is performed by heating seed at 41 o C for 48 hr at 80% RH prior to germination testing. This treatment resulted in a 0 to 50% reduction in the observed germinative energy. The objective of this study is to evaluate the utility accelerated seed aging to predict the long term storage viability of barley. The study was initiated with the 2004 crop, and sample collection will be continued for through 2006. Where possible, samples will be selected to include some sprouting. Storage is at room temperature (24 o C), 7 o C, and under outdoor ambient conditions. Germinative will be determined 3 times each year, and for a total of 3 years storage. Accelerated aging is performed, as described above, following harvest. Data for the 2004 -2006 samples is shown in Table III. Table III Germination and Sprouting Parameters of the 2004 - 2006Barley Samples Selected for Study of Storage Viability (data is 4 months post-harvest) Germinative Energy (4 ml, %) 2004 2005 2006 2004 Accelerated Aging Germinative Energy (%) 2005 2006 2004 Water Sensitivity (4-8 ml, %) 2005 2006 2004 RVA Stirring Number 2005 2006 N 15 15 15 15 15 15 15 15 15 15 15 15 Mean 92 95 98 72 86 93 33 24 7 101 127 135 Minimum 85 77 84 8 77 40 9 4 0 5 65 4 Maximum 100 100 100 93 100 100 75 48 40 216 191 242 (B) Barley CAP Project (Dr Yin Li). We are screening 960 breeder’s lines for betaglucanase activity as part of the Barley CAP Project (Barley Coordinated Agricultural Project). Lines will be screened at low and elevated temperatures to detect possible differences in thermostability. A subset of the 960 lines will also be analyzed for lipoxygenase activity. The barley Coordinated Agricultural Project (CAP) is a community effort of 30 scientists from 19 institutions (http://barleycap.coafes.umn.edu/ ). The University of Minnesota is 87
Page 1 and 2:
Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
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EARLY GENERATION LINES Forty F5 pop
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have evaluated a PCR based marker,
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Edward Schiefelbein, Research Scien
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small grains pathology input to thi
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Project Personnel Small Grains Path
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Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74: 6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76: Objectives Met in the One-Year Fund
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102: Kevin Hicks, Research Leader, USDA-
Page 103 and 104: Detailed Report on
Page 105 and 106: Other Barley Research and Future Di
Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
Page 119 and 120: horseradish peroxidase-conjugated g
Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125: Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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