Annual Progress Report on Malting Barley Research March, 2007

induced to sporulate followed by a second round of single sporing to ensure purity.
Phenotypic data was collected by inoculating each field isolate on each of the 22
barley lines. The barley cultivar Heartland was added to the set in 2005 (Table 1)
and therefore no data is available for 2004 collections. For 2005 the differential set
was inoculated with conidia of 25 individual isolates collected at Langdon and 10
isolates collected at Fargo. Inoculations were completed at the two-to-three-leaf
stage as follows. Individual differential lines were planted along with controls using
three conetainers (Stuewe and Sons, Inc., Corvallis, OR) per line and three plants
per conetainer. Conidia were grown and harvested as described by Weiland et al.
(1999). Conidia were diluted to 3000 spores/ml, and 2 drops of Tween 20
(polyoxyethylene sorbitan monolaurate) was added per 100 ml of inoculum. Plants
were inoculated to a heavy mist but not to runoff. Following inoculation, plants were
placed in 100% relative humidity at 21˚C for 24 h, and then placed in a controlled
chamber under a 12 h photoperiod at 21˚C. Disease readings were taken at 7 d
post-inoculation using the 1-10 scale developed by Tekauz (1985) where average
disease reactions of less than 5 were considered resistant and those 5 or greater
were considered susceptible. At least two replicates were completed for each
isolate collected.
Results
The comparison of 2004 and 2005 virulence data showed some striking changes in
virulence patterns found across the isolates tested. The 2004 data showed that
resistance found in Canadian Lake Shore, CI4922, Harbin, Manchuria, Manchurian
and Tifang were not effective against any of the isolates collected at either location
(Table 1). In 2005 this was also the case for isolates collected in Fargo whereas in
Langdon these sources of resistance were much more effective indicating a shift
away from virulence on these
barley lines in the Langdon area
(Table 1). Barley lines Algerian,
CI5791, CI9214, NDB112, and
Prato showed high levels of
resistance in both years whereas
lines Rika and SM89010 had a
reduction in effective resistance
in 2005 especially in the Langdon
collections.
Barley lines NDB112 and
Heartland are used as disease
resistance sources in the North
Dakota and Minnesota barley
breeding programs respectively.
Both of these lines look to be very
effective against the current
pathotypes, with both lines
94
%
%
%
%
virulence virulence virulence virulence
Fargo, ND Langdon, ND Fargo, ND Langdon,
2005
2005
2006
ND
CI
(10 isolates) 2006
Barley line number
(25 isolates)
Algerian 1179 0 0 0 0
Atlas 4118 32 40 90 0
Beecher 6566 24 20 70 0
Can. Lk. Sh. 2750 100 100 100 0
Cape 1026 80 60 90 84
CI11458 11458 92 40 80 0
CI4922 4922 100 100 100 0
CI5791 5791 0 0 0 0
CI9214 4929 0 0 0 0
Harbin 12673 100 100 100 0
Hazera 145351 20 12 90 0
Heartland 552963 0 0
Hector 15514 100 100 100 100
Kombar 15694 32 40 100 68
Manchuria 2330 100 100 100 60
Manchurian 739 100 100 100 56
Ming 4797 100 88 80 0
NDB112 11531 12 4 0 0
Prato 15815 4 0 0 4
Rika 8069 20 20 30 84
SM89010 4 12 0 60
Tifang 4407-1 100 100 100 0