Annual Progress Report on Malting Barley Research March, 2007

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derived from the backcross: Strider/88Ab536//88Ab536. STAB 47 and STAB 113 are doubled haploid lines from the cross of Strider/88Ab536. KAB 50 and KAB 51 and KAB lines are doubled haploid lines from the cross of Kold/88Ab536. Quality: Quality data from the 2006 harvest have not been received, due to the movement of the CCRU to a new facility. Past data on the three lines in first year AMBA Pilot, plus a fourth selection that will be submitted in 2007, are summarized in Table 3. These data show a pattern of lower grain protein (and consequently lower enzyme activity) but as is apparent from the pre-screening data from Aberdeen, ID, these selections can achieve high grain protein. It remains to be seen what happens to extract and enzyme activity at these high levels. An advantage of winter over spring barley will be nitrogen management options. Fall/Spring split applications of N will allow for more precise targeting of protein and enzyme levels. Agronomics: The selections all have high test-weight and a high percentage of plump seed. The yield potential is truly impressive, as is shown in Table 4. Irrigated winter barley will save at least one application of scarce and expensive water compared to spring barley grown at the same location. Disease data, as compared to check varieties, are shown in Table 5. All selections showed excellent stripe rust resistance. No BYDV symptoms were reported on any of the four selections at UC Davis nor observed at Corvallis. The selections show a range of reaction to scald under intense disease pressure at Corvallis – such intense scald levels are usually not observed in target production areas. Winter hardiness: We obtained very useful winter hardiness data from two locations. The data on selections are shown in Table 6 and perspectives on the entire germplasm array are shown in Figure 1. Two features stand out. One is that we have a limited number of winter x spring cross progeny with good winter hardiness profiles. Since both the high and low lines are part of the CAP set, we will have complete genotype data on this germplasm, allowing new insights into the genetic control of winter hardiness. This should further facilitate the conversion of elite spring malting lines to winter habit. Secondly, a set of elite winter lines from Europe was included in the controlled freeze tests in Hungary. It is gratifying that our germplasm is competitive with the best European material. Advanced, preliminary, and early generation materials These nurseries, and the numbers of lines therein, are shown in Table 1. 104
Other Barley Research and Future Direction of Program In addition to winter malting barley development, the Oregon Barley Project is engaged in a number of other endeavors: Basic research: • Winter hardiness genetics • Barley CAP Applied research • Winter barley for human nutrition • Barley straw for algae control We plan to continue these areas of endeavor in the future. Project Personnel Patrick Hayes, Professor Jennifer Kling, Senior Research Professor Peter Szucs, post-doc Ann Corey, Senior Research Assistant Tanya Filichkin, Senior Research Assistant Kale Haggard, Graduate Research Assistant Juan Rey, Graduate Research Assistant Phinyarat Kongprakhon, Visiting Scholar 105 Recent Publications (2006 - 2007) 1. Druka, A., G. Muehlbauer, I. Druka,, R..Caldo, U. Baumann, N. Rostoks, A. Schreiber, R. Wise, T. Close, A. Kleinhofs, A. Graner, A. Schulman, P. Langridge, K. Sato, P. Hayes, J. McNicol, D. Marshall, and R. Waugh. 2006. An atlas of gene expression from seed to seed through barley development. Func. Int. Genomics. 6:202-211. 2. Szűcs, P., I. Karsai, J. von Zitzewitz, K. Mészáros, L.L.D. Cooper, Y.Q. Gu, T.H.H. Chen, P.M. Hayes, and J.S. Skinner. 2006. Positional relationships between photoperiod response QTL and photoreceptor and vernalization genes in barley. Theor. Appl. Genet. 112:1277-1285. 3. Richardson K.L,, M. I. Vales, J. G. Kling, C. C. Mundt, and P. M. Hayes. 2006. Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theor. Appl. Genet. 113:485-495. 4. Yun, S.J., L. Gyenis, E. Bossolini, P.M. Hayes, I. Matus, K.P. Smith, B.J. Steffenson, R. Tuberosa and G. J. Muehlbauer. 2006. Validation of quantitative trait loci for multiple disease resistances using advanced backcross lines developed with a wild barley (Hordeum vulgare subsp. spontaneum). Crop Sci. 46: 1179-1186.
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Annual Pro
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-i- INTRODUCTION The March 2007 <st
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USDA-ARS NORTHERN CROP SCIENCE LABO
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Spring Regional over two years at D
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Table 1. 2006 UCD Two-Rowed Malting
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environments was practiced, 2) elit
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Table 4. Selected six-rowed lines c
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Other Barley Research: Feed Program
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Executive Summary AMBA Prog
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Table 1. Agronomic performance of M
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Lines M122 and M124 were rated sati
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EARLY GENERATION LINES Forty F5 pop
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have evaluated a PCR based marker,
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Edward Schiefelbein, Research Scien
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small grains pathology input to thi
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Project Personnel Small Grains Path
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Investigations on Barley Diseases a
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Minnesota barley disease survey and
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pathotypes at the seedling stage in
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germplasm with the highest level of
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Mirlohi, A., Brueggeman, R., Steffe
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Developing Improved Malt Barley Var
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Table 2. Montana’s Statewide Tria
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As a Barley CAP collaborator, our C
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3.2. A barley nursery composed of d
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5. Related Barley Projects Barley P
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Page 55 and 56: Table 3. Agronomic comparisons of N
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Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
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Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
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Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
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Page 107 and 108: Table 1. 2006/2007 Oregon winter ba
Page 109 and 110: Table 4. Agronomic data for OSU win
Page 111 and 112: MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114: We recently secured an industrial c
Page 115 and 116: 2006-2007 Progress
Page 117 and 118: enzyme it encodes. We found that se
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Page 121 and 122: found in plant cell walls. The degr
Page 123 and 124: Establishing that sequence variatio
Page 125: Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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