Annual Progress Report on Malting Barley Research March, 2007

More documents

Recommendations

Info

Two first year entries, M128 and M129, were submitted to AMBA pilot malt evaluation with the 2006 crop. Both of these lines are from our FHB breeding program and have levels of resistance equal to or greater than MNBrite. M128 has the resistance sources Atahualpa, MNBrite and Zheddar in its pedigree. M128 is slightly higher in yield and similar in lodging to Robust. It is intermediate in height to Robust and Lacey with about a 30% reduction in FHB severity and DON compared to Robust (Table 6). Table 6. Agronomic and FHB performance of M128 compared to check varieties 2003- 2006. Days to Plant FHB Variety/Line Yield Lodging Heading Height Severity DON (Bu/A) (%) (Days) (Inches) (% of Robust) (% of Robust) Sta. Yrs. 14 3 14 14 10 11 M128 101 34 57 33 65 73 Robust 100 38 57 35 100 100 Stander 103 36 57 31 135 157 Lacey 102 35 57 31 -- -- MNBrite -- -- -- -- 84 75 M129 has the resistance source AC Oxbow in its pedigree. M129 is equal in yield, slightly shorter, and slightly better in lodging resistance compared to Robust. M129 exhibits about a 50% reduction in disease severity and 23% reduction in DON (Table 7). Table 7. Agronomic and FHB performance of M129 compared to check varieties 2003- 2006. Days to Plant FHB Yield Lodging Variety/Line Heading Height Severity DON (Bu/A) (%) (Days) (Inches) (% of Robust) (% of Robust) Sta. Yrs. 14 3 14 14 10 10 M129 100 30 57 34 52 77 Robust 100 38 57 35 100 100 Stander 103 36 57 31 156 153 Lacey 102 35 57 31 -- -- MNBrite -- -- -- -- 83 83 INTERMEDIATE AND PRELIMINARY YIELD TRIALS Fourty-five lines were grown and evaluated in our intermediate yield trials at 3 locations (St. Paul, Morris, and Crookston). Nineteen of these lines have partial resistance to FHB and another three have enhanced resistance to Septoria and one to net blotch. Our preliminary yield trials grown at St. Paul, Morris, and Crookston included 183 lines. Of these lines, 94 came from our FHB screening program and have partial resistance to FHB. 22
EARLY GENERATION LINES Forty F5 populations were grown at Crookston and St. Paul. The populations were largely of two types, i.e., traditional elite by elite and elite by FHB resistant. Nine of the F5 populations (elite by elite) along with eight populations segregating for resistance to net blotch or septoria were advanced through the F3 and F4 generations via single seed descent (SSD) in greenhouses in fall and winter 2005-2006. These populations were planted as F5 head rows in Crookston or Stephen (net blotch) in the summer of 2006. Selection in these crosses was based on heading date, height, lodging, kernel plumpness, and spike characteristics. The Septoria and net blotch populations were planted in inoculated nurseries. We were unable to get good Septoria disease expression and therefore unable to select for resistance. These lines were screened for resistance to Septoria in the Fall greenhouse and resistant lines will be advanced to 2007 trials. Disease pressure at our net blotch nursery was light, so we also conducted greenhouse disease screening in the Fall. Twenty-four of the F5 populations (elite by FHB) were advanced to F3 in a fall 2005 greenhouse in Minnesota and then to the F4 as single plants in Christchurch, New Zealand. The New Zealand nursery allowed us to harvest enough seed from individual plants for replicated FHB nurseries in a timely manner for planting. Selection on FHB populations in the 2006 field season was based on FHB resistance, heading date, height, and lodging. Grain harvested from selected rows was analyzed for DON. In addition, we plant a single row outside of the disease nursery for malting quality analysis in Madison, WI. Heads from selected F5’s in 2006 were planted in single rows in a winter nursery (2006/2007) in Yuma, AZ to produce seed for 2006 PYTs. Based on 2006 FHB, DON, and malting quality data and also phenotype in the Yuma nursery, about 200 lines will be selected for entry to preliminary yield trials in 2007. A total of 51 F2 populations were grown in the field at St. Paul and Crookston in 2005. In addition, we grew eight F2 populations in the summer greenhouse in cones arranged in a 96-well format to facilitate marker assisted selection (MAS). In these populations, we selected for markers linked to a QTL identified on chromosome 6(6) (Wingbermuehle et al., 2004) and markers linked to a QTL for resistance to Septoria speckled leaf blotch. Marker genotyping was done in collaboration with Dr. Shiaoman Chao at the USDA Genotyping Lab in Fargo, ND. SCREENING FOR DISEASE RESISTANCE FHB Steady progress has been made in breeding for resistance to FHB. Funding through the US Wheat and Barley Scab Initiative and the Minnesota Small Grains Initiative have allowed us to sustain a major effort in disease resistance breeding and studies aimed to understand and exploit the genetics of disease resistance. Our FHB screening effort is conducted in collaboration with Dr. Ruth Dill-Macky and Dr. Charla Hollingsworth. We are advancing resistant lines in our program tracing to different sources of resistance and continue to make crosses with new sources of resistance as they are identified. We have been working with Dr. Brian Steffenson in selecting new sources of FHB resistance to introduce into the breeding program. Each year we have increased the number of entries 23
Page 1 and 2: Annual Pro
Page 3 and 4: -i- INTRODUCTION The March 2007 <st
Page 5 and 6: USDA-ARS NORTHERN CROP SCIENCE LABO
Page 7 and 8: Spring Regional over two years at D
Page 9 and 10: Table 1. 2006 UCD Two-Rowed Malting
Page 11 and 12: environments was practiced, 2) elit
Page 13 and 14: Table 4. Selected six-rowed lines c
Page 15 and 16: Other Barley Research: Feed Program
Page 17 and 18: Executive Summary AMBA Prog
Page 19 and 20: Table 1. Agronomic performance of M
Page 21: Lines M122 and M124 were rated sati
Page 25 and 26: have evaluated a PCR based marker,
Page 27 and 28: Edward Schiefelbein, Research Scien
Page 29 and 30: small grains pathology input to thi
Page 31 and 32: Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49 and 50: 3.2. A barley nursery composed of d
Page 51 and 52: 5. Related Barley Projects Barley P
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74:
6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76:
Objectives Met in the One-Year Fund
Page 77 and 78:
These automated methods have been t
Page 79 and 80:
Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82:
M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84:
Figure 7. Time course of I2-binding
Page 85 and 86:
10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88:
2. Other Research (A) Predicting Se
Page 89 and 90:
forms (through enzymolysis during m
Page 91 and 92:
PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94:
CI9214, PI552963 (Heartland), and N
Page 95 and 96:
showing good levels of resistance t
Page 97 and 98:
Germplasm Enhancement for RWA Resis
Page 99 and 100:
Gary Hein in Colorado and Nebraska.
Page 101 and 102:
Kevin Hicks, Research Leader, USDA-
Page 103 and 104:
Detailed Report on
Page 105 and 106:
Other Barley Research and Future Di
Page 107 and 108:
Table 1. 2006/2007 Oregon winter ba
Page 109 and 110:
Table 4. Agronomic data for OSU win
Page 111 and 112:
MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114:
We recently secured an industrial c
Page 115 and 116:
2006-2007 Progress
Page 117 and 118:
enzyme it encodes. We found that se
Page 119 and 120:
horseradish peroxidase-conjugated g
Page 121 and 122:
found in plant cell walls. The degr
Page 123 and 124:
Establishing that sequence variatio
Page 125:
Charles Karpelenia, technician Joe
show all

Annual Progress Report on Malting Barley Research March, 2007

Create successful ePaper yourself

Delete template?

Save as template?