Annual Progress Report on Malting Barley Research March, 2007

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Table 1. Barley stripe rust reactions of QTL lines, parents and F1 plants from crosses of malting barley cultivars Metcalfe(M) and Legacy(L). # Bison Line QTL BSTR Reaction 1 F1 BSTR Seedling Reaction Sdlg 2 Tllrg 2 Hdg 2 M L 4 69 5 3 R 3 7 7 5 6 128 191 1H 7 4 6 1 R R 7 - 8 - 7 8 104 129 4H 3 4 1 1 R R - - 7 - 9 87 8 7 S 7 - 10 11 111 136 5H 8 7 7 8 I I 7 3 7 7 12 217 4 2 I - 3 13 2-22 7H 0 1 R 0 14 216-4 4 1 R - 7 15 243-4 1Hx4H 5 3 I - 7 16 136-2 4 1 R - - 17 18 218-1 110-3 1Hx5H 5 6 1 5 R I - - - 7 19 20 217-2 22-4 4Hx5H 4 4 1 2 I R - - 7 7 21 95-2 1Hx4Hx5H 4 1 R - 7 22 Metcalfe Malting Parent 8 - - - - 23 Legacy Malting Parent 8 - - - - 24 Baronesse 7 - - - - 25 BCD12 4 - - - - 26 BCD47 4 - - - - 1 Scale 0 – 9; 0-3 = resistant, 4-6 = intermediate, 7-9 = susceptible 2 Sdlg = seedling; Tllrg = tillering; Hdg = heading 3 R = resistant, I = intermediate, S = susceptible 4.2. Barley stripe rust was not observed in any nursery in 2006, although we experienced a severe outbreak of wheat stripe rust on susceptible cultivars in western and central Montana. Stripe rust of wheat can over-winter on winter wheat under snow cover in Montana, whereas winter barley is not grown in the state. Any over-wintering would Thus have to occur on susceptible wild grasses. 4.3. We continue to maintain collections of numerous barley pathogen; Puccinia hordei, leaf rust; Puccinia graminis ,stem rust; Puccinia striiformis hordei, stripe rust; Rhynchosporium secalis, scald; Pyrenophora teres, net blotch and Erysiphe graminis, powdery mildew. All cultures with the exception of powdery mildew are preserved by various means. Powdery mildew is maintained on living plants in isolation in a cold room at 40F. 50
5. Related Barley Projects Barley Portion of USDA/ARS stripe rust initiative: Precise phenotyping of barley stripe rust resistance gene introgression. Barley elite lines or cultivars from areas at greatest risk for stripe rust development (Montana, California, Idaho and Washington) are being utilized for gene introgression. Numerous crosses have been made by cooperators. First F1 populations are currently being screened at MSU. 6. Publications Richardson, K., Chen, X., Johnston, M., Capettini, F Vales, I., Kling, J., Mundt, C., and Hayes, P. Barley stripe rust resistance QTL alleles are effective across growth stages, races, and environments. Submitted to Phytopathology. 51
Page 1 and 2: Annual Pro
Page 3 and 4: -i- INTRODUCTION The March 2007 <st
Page 5 and 6: USDA-ARS NORTHERN CROP SCIENCE LABO
Page 7 and 8: Spring Regional over two years at D
Page 9 and 10: Table 1. 2006 UCD Two-Rowed Malting
Page 11 and 12: environments was practiced, 2) elit
Page 13 and 14: Table 4. Selected six-rowed lines c
Page 15 and 16: Other Barley Research: Feed Program
Page 17 and 18: Executive Summary AMBA Prog
Page 19 and 20: Table 1. Agronomic performance of M
Page 21 and 22: Lines M122 and M124 were rated sati
Page 23 and 24: EARLY GENERATION LINES Forty F5 pop
Page 25 and 26: have evaluated a PCR based marker,
Page 27 and 28: Edward Schiefelbein, Research Scien
Page 29 and 30: small grains pathology input to thi
Page 31 and 32: Project Personnel Small Grains Path
Page 33 and 34: Investigations on Barley Diseases a
Page 35 and 36: Minnesota barley disease survey and
Page 37 and 38: pathotypes at the seedling stage in
Page 39 and 40: germplasm with the highest level of
Page 41 and 42: Mirlohi, A., Brueggeman, R., Steffe
Page 43 and 44: Developing Improved Malt Barley Var
Page 45 and 46: Table 2. Montana’s Statewide Tria
Page 47 and 48: As a Barley CAP collaborator, our C
Page 49: 3.2. A barley nursery composed of d
Page 53 and 54: Barley Breeding - Advanced Testing
Page 55 and 56: Table 3. Agronomic comparisons of N
Page 57 and 58: Preliminary Malting Barley Yield Tr
Page 59 and 60: dwarf genotypes with the convention
Page 61 and 62: STUDIES ON BARLEY DISEASES AND THEI
Page 63 and 64: inoculated plots. The inoculum used
Page 65 and 66: much of North Dakota in 2006, few f
Page 67 and 68: 5. Collect isolates of barley patho
Page 69 and 70: oot rot. In collaboration with Scot
Page 71 and 72: 2. United States Wheat and Barley S
Page 73 and 74: 6. Lee, S. and Neate, S.M. (2006) P
Page 75 and 76: Objectives Met in the One-Year Fund
Page 77 and 78: These automated methods have been t
Page 79 and 80: Beta-limit dextrin Alpha-Amylase Al
Page 81 and 82: M w kDa 800 600 400 200 0 0.4 0 2 4
Page 83 and 84: Figure 7. Time course of I2-binding
Page 85 and 86: 10 9 8 7 6 5 4 3 2 1 0 1993 1994 19
Page 87 and 88: 2. Other Research (A) Predicting Se
Page 89 and 90: forms (through enzymolysis during m
Page 91 and 92: PUBLICATIONS (2006-07) Schwarz, P.B
Page 93 and 94: CI9214, PI552963 (Heartland), and N
Page 95 and 96: showing good levels of resistance t
Page 97 and 98: Germplasm Enhancement for RWA Resis
Page 99 and 100: Gary Hein in Colorado and Nebraska.
Page 101 and 102:
Kevin Hicks, Research Leader, USDA-
Page 103 and 104:
Detailed Report on
Page 105 and 106:
Other Barley Research and Future Di
Page 107 and 108:
Table 1. 2006/2007 Oregon winter ba
Page 109 and 110:
Table 4. Agronomic data for OSU win
Page 111 and 112:
MALTING QUALITY ANALYSIS OF NEW BAR
Page 113 and 114:
We recently secured an industrial c
Page 115 and 116:
2006-2007 Progress
Page 117 and 118:
enzyme it encodes. We found that se
Page 119 and 120:
horseradish peroxidase-conjugated g
Page 121 and 122:
found in plant cell walls. The degr
Page 123 and 124:
Establishing that sequence variatio
Page 125:
Charles Karpelenia, technician Joe
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Annual Progress Report on Malting Barley Research March, 2007

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