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Table of Contents
Chapter 1: Introduction 1
Objectives 4
Chapter 2: Materials and methods 6
2.1 Localities and voucher specimens of plants collected 6
2.2 Ethnobotanical data 6
2.2.1 Plants information collected from the survey 9
2.3 Bacteria used in the study 12
2.4 List of chemicals and reagents 14
2.5 Standard experimental procedures 15
2.5.1 Preparation of plant extracts 15
2.5.2 Culture and media preparation 15
2.5.3 Antibacterial assay 16
2.5.3.1 Agar well diffusion assay 16
2.5.3.2 Disc diffusion assay 17
2.5.3.3 Serial dilution for determination of the minimal inhibitory
concentration (MIC) 17
2.6 Phytochemical screening 18
2.6.1 Alkaloids 18
2.6.2 Flavonoids 18
2.6.3 Saponins 18
2.6.4 Anthraquinones 19
2.6.5 Cardiac glycosides 19
2.6.6 Tanins 19
2.7 Bio-autographic assay 20
Chapter 3: Ethnobotany 21