TiHo Bibliothek elib - Tierärztliche Hochschule Hannover

Untersuchung des Chemokins MIP-3 β/CCL19
chemotactic activity for mononuclear cells in the migration assay, proving that the
measured CSF protein is available in the active form.
Conclusions: The hypothesis was confirmed that MIP-3β/CCL19 is involved in the
pathogenesis of SRMA/MUO, possibly perpetuating an autoimmune reaction.
The marked chemotactic activity for mononuclear cells leads to the assumption that
this chemokine could be associated with the migration of mononuclear cells into the
subarachnoid space of dogs. The elevation of CSF MIP-3β/CCL19 in IVDD suggests
that it also plays a role in the secondary wave in spinal cord injuries (SCI). CSF MIP-
3β/CCL19 concentration may serve as a prognostic indicator in SCI, be a precious
biomarker for developing new treatment schemes and for verifying the success of
treatment.
1. Introduction
In several neurologic diseases a pleocytosis is often detected in the cerebrospinal
fluid (CSF). Lymphocytes and plasma cells have been shown to prevail in the cell
count in viral infections (Wamsley and Alleman 2004), chronic steroid responsive
meningitis-arteritis (SRMA) (Tipold and Jaggy 1994), granulomatous
meningoencephalomyelitis (GME) and in necrotizing encephalitides (NE) (Wamsley
and Alleman 2004; Talarico and Schatzberg 2010). A neutrophilic pleocytosis is
characteristic for bacterial infections and the acute stage of SRMA (Tipold and Jaggy
1994). A mixed cell population can be seen in protozoal diseases, chronic bacterial
infections, necrotic lesions, and in GME (Wamsley and Alleman 2004). In addition to
inflammatory lesions, mild mixed cell pleocytosis can also be seen with situations
such as acute intervertebral disc herniation that results in central nervous system
(CNS) myelomalacia or infarction (Chrisman 1992).
Chemokines are important factors in the mechanism of cell migration and thus play a
relevant role in the pathogenesis of inflammation in the CNS (Dogan and Karpus
2004). They upregulate surface molecules on circulating inflammatory cells in the
periphery which enable them to more efficiently adhere to the endothelial cells of the
blood-brain barrier (BBB) and react to the CNS chemokine gradients (Constantin
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