TiHo Bibliothek elib - Tierärztliche Hochschule Hannover

Untersuchung des Chemokins MIP-3 β/CCL19
mean CCL 19 concentration showed a pronounced impact on cell migration, which
indicates the presents of a functional chemotactic protein. This function of CCL19
might play an important role in secondary damages in spinal cord injuries (SCI).
A study from Pineau and Lacroix (2007) on rodent models revealed that cytokine
production is time dependent. CNS resident cells, including neurons, synthesized
cytokines (IL-1, TNF, IL-6, and leukemia inhibitory factor) between 3 and 24 hours
post SCI and some infiltrating leukocytes were responsible for the cytokine
production 12 hours after the trauma. This would suggest that neural cells are
responsible for the initial inflammatory response following SCI but, the recruitment of
additional immune cells is responsible for the maintenance of inflammation (Pineau
and Lacroix 2007). Additional studies would be necessary to determine the time at
which CCL19 becomes a major inflammatory mediator.
Kwon et al. observed in his study that IL-6, IL-8, MCP-1, tau, S100b and glial fibrillary
acidic protein (GFAP) were elevated depending on severity of the initial injury.
Evaluating this group of inflammatory cytokines a biochemical model was developed
enabling to predict the ASIA (American Spinal Injury Association) grade in 89% of
their subjects. It was found that the pattern of protein expression within the CSF over
the first few days following injury were strong indicators of neurologic outcome and
thus, are now a focus of therapeutic research for SCI (Kwon et al. 2010).
Using CCL19 CSF concentration as a prognostic marker could be also a precious
tool in the research of SCI in dogs, which should be followed up in further studies.
In conclusion CCL19 may play an important role in the pathogenesis of SRMA/ MUO,
possibly perpetuating an autoimmune reaction and may also be involved with the
secondary wave of spinal cord injuries (SCI) in IVDD. The marked chemotactic
activity for mononuclear cells leads to the assumption that this chemokine could be
associated with the migration of mononuclear cells into the subarachnoid space of
dogs. CCL19 concentration therefore, may serve as a prognostic indicator in SCI, be
a precious biomarker for developing new treatment schemes and for verifying the
success of treatment.
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