TiHo Bibliothek elib - Tierärztliche Hochschule Hannover

Summary
Kapitel 8 Summary
Janina Bartels: Detection of the Chemokine MIP-3β/CCL19 in cerebrospinal fluid
and its association with the invasion of mononuclear cells in
neuroinflammatory and non-neuroinflammatory diseases in dogs.
Chemokines are important factors for the cell migration and pathogenesis of
inflammatory reactions in the central nervous system (CNS). They can be upregulated
in tissues under pathologic conditions. Certain chemokine gradients can
influence the cell migration and lead to the activation of immune cells. The first
aspect of this study covered the quantification of chemokine MIP-3β (macrophage
inflammatory protein-3 beta)/CCL19 concentrations in the cerebrospinal fluid (CSF)
and serum samples in dogs with inflammatory and non-inflammatory diseases of the
central nervous system (CNS).
The second aspect of this study addressed the determination of the chemotactic
activity of mononuclear cells induced by CSF containing elevated MIP-3β/CCL19
concentrations. The hypothesis should be proven that MIP-3β/CCL19 concentrations
are increased in CSF samples of selected neurological diseases and that higher
concentrations of MIP-3β/CCL19 within the CNS induce mononuclear cell migration.
MIP-3β/CCL19 concentration measurements were performed using a commercially
available ELISA in 141 paired serum and CSF samples of dogs. Elevated MIP-
3β/CCL19 values could be measured in CSF samples of dogs with inflammatory and
non- inflammatory neurological diseases. MIP-3β/CCL19 CSF concentrations in dogs
with steroid-responsive meningitis-arteritis (SRMA) and meningoencephalitides of
unknown origin (MUO) were significantly higher compared to the control group
consisting of healthy dogs and patients with idiopathic epilepsy (p < 0.001 ; median
CCL19 concentration SRMA: 1690 pg / ml; MUO: 373.5 pg / ml).
Additionally, a significant correlation between MIP-3β/CCL19 CSF concentrations
and CSF cell count in dogs affected with SRMA and MUO (SRMA r = 0.82, p