2110XP Ammonia Analyzer User Guide (1574 Kb) - Thermo Scientific

Calibration
Before Performing
DKA Calibration
Recommendations
Maximum system accuracy is ensured through a fast, easy, and accurate
calibration performed in the expected sample range. This calibration
procedure uses equipment supplied in the calibration kit (Cat. No.
2110XPCAL).
• Wait at least one hour after changing reagent.
• Check that the present sample concentration is equal or less than
standard 1; if not, calibration can still be performed with a loss in
accuracy as long as background concentration is not significantly larger.
The best results are achieved when standard 1 is twice the level of the
present sample concentration.
• Proper pipette technique is crucial to calibration. If user is not familiar
with pipette equipment or technique, refer to Appendix A-11, Pipette
Operation.
• Do not touch pipette tip with bare fingers. Risk of contamination is
possible. Use powder free gloves to prevent contamination.
• When using a pipette to inject standards during calibration, use a fresh
pipette tip for each standard and each injection.
• When a fresh tip is installed, rinse the tip with the same concentration
standard as the intended injection.
• Fill the tip and dispense the standard 1-3 times before injecting the
standard into the flow cell. Be sure to discard the standard in the
drain between the rinses.
• Wipe any excess liquid from the outside of the tip with out touching
the opening.
• Insert the pipette tip into calibration port on flow cell cap.
Figure IV-4
Pipette Dispensing
• Be sure to hold the pipette completely vertical while dispensing. Do
not inject the standard on the side of the flow cell, as a film may be
retained on the inside of the wall, resulting in errors in precision of
calibration. Refer to Figure IV-4.
Thermo Scientific Orion 2110XP Ammonia Monitor User Guide
IV-5