第117å›žæ—¥æœ¬è§£å‰–å­¦ä¼šç·ä¼šãƒ»å ¨å›½å­¦è¡“é›†ä¼š 講演プログラム・抄録集 PDF ...

117 117
OFPMVIII
α
NPC1L1
LC3II
NPC1L1
Lamp1 Ragulator
p18 Lamp1 mTOR
α1 ATZ
α1
OFPMVIII
E HRD
ERassociated
degradation; ERAD unfolded
protein response; UPR E3
HRD1 ERAD UPR
ERAD UPR
HRD1
ICR 6 4%
HRD1 Abgent1.25 μg/ml
HRD1
HRD1
OFPMVIII
1 2 2
1
2
[Aim] We investigated the influence of the adrenal glands over the preservation
of gap junctions between folliculostellate cells in the anterior pituitary glands of
rats. [Maerials and Methods] 60 dayold WistarImamichi strain male rats were
prepared. The animals were divided into six groups: untreated control, untreated
but given 0.9 % NaCl drinking water, castrated, adrenalectomized, castrated and
adrenalectomized, and castrated and adrenalectomized with the administration
of testosterone. Five rats from each group were killed 1, 2, 3, 4, 5, 6 and 7
days after the operation, and prepared for observation by transmission electron
microscopy. [Results] The simultaneous removal of adrenal glands with castration
resulted in the significantly decreasing effects of gap junctions, and the additional
administration of testosterone to these rats could compensate for the decreasing
effects of gap junctions, while few mitotic hormoneproducing granular cells
were found. [Conclusion] These observations indicate that the preservation of gap
junctions between folliculostellate cells is mainly dependent on the testosterone
from both testes and adrenal glands in adult male rats.
OGPMIV
NEX
NEX Math2 bHLHtype transcriptional factor
Cre
NEX
OGPMIV
FLRT Unc
1,2 Falko Hampel 2 3 Daniel del Toro 2
Manuela Schwark 4 Elena Kvachnina 4 Martin Bastmeyer 5 3
Victor Tarabykin 4 Joaquim Egea 2 Ruediger Klein 2
1
2 MaxPlanck Institute of
Neurobiology 3 4 MaxPlanck
Institute for Experimental Medicine 5 Karlsruher Institute fuer Technologie
FLRT
Unc5
FLRT2 Unc5D UL2
Unc5D UL2
RNA
FLRT2 VVI UL2
IV Unc5D
FLRT2/ UL2
FLRT2 UL2
OGPMIV
Implications for cooperative versus noncooperative actions of the
subunits of NatB, Mdm and Nat in mouse embryonic brain
Kyoji Ohyama 1 , Kunihiko Yasuda 1 , Kazuko Onga 1 , Akira Kakizuka 2 ,
Nozomu Mori 1
1
Dept. of Anatomy and Neurobiology, Grad. Sch. Biomedical Sciences, Nagasaki
Univ., 1124 Sakamoto, Nagasaki 8528523, Japan, 2 Lab. of Functional Biology,
Kyoto Univ. Grad. Sch.Biostudies, Kyoto 6068501, Japan
The NatB complex, Nat5/Mdm20 acetyltransferase mediates Nacetylation to
control cell cycle progression and actin dynamics in yeast. As yet, little is known
about their expression patterns in multicellular organisms. Here we show that
Mdm20 is highly expressed in mouse embryonic brain. At E11.5, Mdm20 was
widely expressed in both neural progenitors and early differentiating neurons,
whereas Nat5 was expressed in Sox1/3+/Mdm20+ neural progenitors. By E14.5,
both Mdm20 and Nat5 were downregulated in ventricular zone neural progenitors,
whereas both protein expression were found in differentiating neurons and
maintained at E18.5 in derivatives of these cells, such as midbrain dopaminergic
DA neurons. Intriguingly, our data also showed that Mdm20 is not always co
expressed with Nat5 in all differentiated neurons, for example deep cerebellar
neurons. Moreover, Mdm20 is also not necessarily colocalised with Nat5 within
Nat5+/Mdm20+ midbrain DA neurons. Given that Nat5 is only a known member
of Nat family protein that interacts with Mdm20, our data imply that Mdm20 may
function either with an unidentified Nat protein partners or possibly in a Nat
independent manner.