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140 117 P Characterization of the RNAbinding protein Musashi in zebrafish Musashi Msi is an evolutionarily conserved gene family of RNAbinding proteins RBPs that is preferentially expressed in the nervous system. The first member of the Msi family was identified in Drosophila. Drosophila Msi plays an important role in regulating asymmetric cell division of the sensory organ precursor cells. The mammalian orthologs, including human and mouse Msi1, are neural RBPs that are strongly expressed in neural stem cells NSCs. Mammalian Msi1 contributes to self renewal of NSCs through translational regulation of several target mRNAs. In this study, the zebrafish Msi ortholog zMsi1 was identified and characterized. The normal spatial and temporal expression profiles for both protein and mRNA were determined. Overall, zMsi1 was strongly expressed in neural tissue in early stages of development and exhibited similarity to mammalian Msi1 expression patterns. To reveal the in vivo function of zMsi1, morpholinos against Msi1 were introduced into onecell stage zebrafish embryos. Knock down of zmsi1 frequently resulted in aberrant formation of the CNS. These results suggest that Msi1 plays roles in CNS development in vertebrates. P PSANCAM 1 2 1 1 2 PSANCAMpolysialic acidneural cell adhesion molecule PSANCAM 57 E5E7 PSANCAM PSANCAM BrdU E5E7 BrdU FITC PSANCAM BrdU 10 E6 BrdU E5 BrdU E6 E7 BrdU PSANCAM E5E7 PSANCAM P mRNA 1 2 1 1 2 GnRH mRNA mRNA 2.5 3.5 3.5 4 GnRH mRNA mRNA Tol2GFP 2.5 1619 GFP GnRH GFP mRNA mRNA mRNA GnRH GnRH P CNTF cuprizone MBPCNPase cuprizone MBPCNPase CNTF CNTF P E1313.5 E11.5 Islet1Lim3Lhx3 MNR2 MMCl Lim3 Islet1Lim3Foxp1 E11.5 Islet1 Lim3 Foxp1 P 6
117 141 P kirrel 1 1 1 1 2 1 1 2 A member of the immunoglobulin superfamily, kirrel3, is expressed in the dorsal root ganglia DRGs. In the previous report, we have suggested that kirrel3 may play a role in the development of tyrosine kinase receptor Trk Cpositive neurons. To get further insights into the function of kirrel3 in the DRG neurons, we characterized kirrel3expressing neurons in the DRGs of kirrel3lacZ Z knockin mice. In the lumber DRG neurons, the expression of βgalactosidase βgal was first observed at embryonic day 12.5, gradually increased, and reached the maximum level at postnatal day P 7. During embryonic stages, βgal was predominantly expressed in TrkA and TrkCpositive neurons, while some βgal positive neurons contained TrkB or Ret. The expression of Ret was increased in βgalpositive neurons between P7 and P14, and was observed in approximately 43% of βgalpositive neurons of the adult DRGs. These results suggest that kirrel3 may be involved in the development of nociceptive and mechanoceptive neurons in addition to proprioceptive neurons. This work was supported by a GrantinAid for Scientific Research B from Japan Society for the Promotion of Science 22390036. P BMP4 FGF2 Dlx5Six1Eya2 PCR Dlx5 10 GATA3/keratin19 Slug Sox2 FGF2 FGF8 BMP4 FGF2 P / Sprouty 1,2 2 3 2 1 2 3 in vitro bFGF Receptor tyrosine kinase RTK Sprouty 4 Sprouty in situ hybridization Sprouty4 Sprouty4 Sprouty4 Sprouty4 Sprouty4 P 4 Oct3/4Sox2Klf4cMyc induced pluripotent stem cell iPS iPS P 1 2 3 2 1 2 3 P Dlg 1 1 2 1 2 3 16 Hox11Tcf21 WT1Barx1Nkx3.2 mesothelium Fgf16 FGF receptor12 mesothelium Fgf16 FGF receptor12 Dlg1 MAGUK Dlg1 Dlg1 -/- Dlg1 Dlg1 -/- Dlg1 2010 Wnt Fzd1 2 Dlg1 -/- Fzd1 -/- Fzd2 -/- Dlg1 -/- Fzd1 -/- Fzd2 -/- Dlg1 -/- Notch Notch Dlg1 -/- Dlg1 Notch
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117 3 1 1 1980 4 2002 200
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117 5 100 http://yamanashiko
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117 9 1117TEL: 0552541000 URL:
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26 117 ::B M 15 30
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28 S S S S 117 ex vivo
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30 S 117 Coordinated branching mo
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32 117 ::A M M S. 2
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34 117 ::A M M S. S
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36 117 S kisspeptin S
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38 117 S S ::B M M S.
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40 117 E L C LC ::OEPM I
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42 117 E L C LC ::OEAM III
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44 117 2OHAMIII2 2OHAMIII3 1
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46 117 F L C LC ::OFPM VI 3
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48 117 :: I 1P001 S100A
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50 117 1P073 Makoto Naganuma Acti
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52 117 2P018 Conserved properti
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54 117 2P090 2P091 2P092
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56 117 3P027 3P028 ABC 3P0
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58 117 :: 3P102 3P103
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117 59 100 μm 20 nm 1 μ
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117 61 12 345
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117 63 SM G
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117 65 S Physiological roles of ce
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117 67 S 1,2 1 1 2 JST S
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117 69 S The role of autophagyrela
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117 71 S - ATP ionom
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117 73 S 3D 2 3D 3
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117 75 S 1 2 1 1 1
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117 77 S GSK3β gl
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117 79 S niche DNA endos
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117 81 S GABA GABA Cl KCC2C
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117 83 S By means of retrograd
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117 85 S S
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117 87 S 1,2,3 1 2 CREST, JS
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Page 99 and 100: 117 91 S Focused Ion BeamScanning
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Page 105 and 106: 117 97 W WG 1,2 1 2 WG
Page 107 and 108: 117 99 OEPMI Singleneuron tracing
Page 109 and 110: 117 101 OFPMII 1 2 3 2
Page 111 and 112: 117 103 OHPM Two immunogenic passe
Page 113 and 114: 117 105 OEAMIII PHAL
Page 115 and 116: 117 107 OFAMIV 1 1 1 2
Page 117 and 118: 117 109 OGAMII BrdU Labelr
Page 119 and 120: 117 111 OHAMIII 1 2 2 2
Page 121 and 122: 117 113 ODPMI Large Maf Large
Page 123 and 124: 117 115 OEPMVII 1 1 2 2
Page 125 and 126: 117 117 OFPMVIII α
Page 127 and 128: 117 119 OHPMIV 1
Page 129 and 130: 117 121 OIPMIV 1 2 1 1
Page 131 and 132: 117 123 P SA 1 2 1 2 Glial
Page 133 and 134: 117 125 P γ 4,52 P
Page 135 and 136: 117 127 P cFos 1 2 1 1
Page 137 and 138: 117 129 P Identification of CSPG c
Page 139 and 140: 117 131 P NOS 1 2 4 4
Page 141 and 142: 117 133 P DPEP 1 2 2 1
Page 143 and 144: 117 135 P Activities of human uppe
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Page 153 and 154: 117 145 P FIB/SEM 1,3 1 2
Page 155 and 156: 117 147 P VIP 12 VIP
Page 157 and 158: 117 149 P Motor protein KIFA is es
Page 159 and 160: 117 151 P parvic
Page 161 and 162: 117 153 P SEM 75 mM KC
Page 163 and 164: 117 155 P mAtgAAcGFP mDFCPmCherry
Page 165 and 166: 117 157 P αMangostin 1 2
Page 167 and 168: 117 159 P 1 1 1 1 2 1
Page 169 and 170: 117 161 P planar cell polarity
Page 171 and 172: 117 163 P The metabolism of choles
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Page 177 and 178: 117 169 P Agerelated changes in es
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Page 187 and 188: 117 179 P TIRF ECM ECM 1
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192 117 Marson, Lesley Masahiro,
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194 117 1OFPMI2 1OFPM
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196 117 3P042 2OFAMV2 3OEPM
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198 117 1P015 2P020
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200 117 2OHAMI1 2OHAMIII3 1P0
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202 117 S36 1P097 S95 S52
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204 117 3OEPMVII
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206 117 1P022 2P130
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117 209 117 2012 2 22
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