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146<br />
117 <br />
P<br />
GABA <br />
parvalbumin <br />
1 1 1 1 1 <br />
1 2 3 1<br />
1<br />
2 <br />
3 <br />
MG GABA <br />
parvalbuminPV <br />
<br />
ABC GABA <br />
PV ir GABAir MG<br />
MGd MGv MGm <br />
GABAir <br />
MGm GABAir MGv <br />
MGd MGv PVir calbindinir <br />
MGv MGd PVir <br />
MGv MGm MGm MGv PVir <br />
PVir PVir <br />
MGv MGm GABAir PVir <br />
MGd PVir <br />
GABAir <br />
<br />
<br />
<br />
P<br />
SNARE <br />
<br />
<br />
SNARE <br />
<br />
SNARE <br />
<br />
<br />
<br />
SNARE <br />
<br />
SNARE syntaxin1, SNAP25, VAMP2 <br />
<br />
<br />
<br />
SNARE <br />
<br />
<br />
SNARE <br />
SNARE <br />
<br />
P<br />
GABA <br />
1 2 3 4 2 1<br />
1<br />
2 <br />
3 4 <br />
, <br />
TGNC<br />
SC GABA GABA <br />
GABA NC <br />
GABA NC GABA <br />
DNDS GABA GAT<br />
SC DNDS <br />
NC <br />
GABA <br />
GABA free 24 TG <br />
NC GABA SC GABA <br />
100 μM GABA 24 NC <br />
SC GABA GABA GAD<br />
GABA GAT NC SC<br />
<br />
NC GAT GABA SC <br />
GABA <br />
GABA <br />
NC SC <br />
<br />
P<br />
Expression of AMPA type glutamate receptor in developing chick<br />
vestibulocochlear ganglia<br />
<br />
1<br />
Expression of AMPA type glutamate receptor GluR14 in the vestibular VG<br />
and cochlear ganglia CG in developing chick embryo was examined. VG and<br />
CG at the embryonic day 10 E10, E12, E14, E16, E18 and E20 were prepared<br />
for reverse transcriptasePCR RTPCR and immunohistochemistry IHC. In<br />
the RTPCR study, GluR1 mRNA was weakly expressed from E10 to E20 in both<br />
ganglia. Expression of GluR2 mRNA was intensive at E10 and E12 in VG and at<br />
E10E14 in CG, and then, it was gradually weakened from E14 in VG and from<br />
E16 in CG. Expression of GluR3 mRNA in both ganglia was weak at E12E18<br />
and it was extremely augmented at E20. GluR4 mRNA was moderately expressed<br />
at E10E14 in VG and at E10E16 in CG, and then, it was gradually increased<br />
from E16 in VG and from E18 in CG. In the IHC study, immunoreactivity of<br />
GluR2 was observed in the cytoplasm and process and that of GluR2/3 and<br />
GluR4 was in the cytoplasm of the VG and CG cells, while that of GluR1 was<br />
never detected. These results suggest that GluR2, GluR3 and GluR4 are the major<br />
subunits of AMPA receptor during the development of the chick embryo VG and<br />
CG cells. They would have different roles for the development.<br />
P<br />
<br />
<br />
1 Mohammad Rabiul Karim 1,2 1<br />
1<br />
2 Faculty of Veterinary Science,<br />
Bangladesh Agricultural University<br />
<br />
<br />
3 <br />
VGLUT 1 <br />
<br />
<br />
1 <br />
VGLUT <br />
<br />
<br />
RTPCR in situ hybridization RT<br />
PCR 2 3 VGLUT 3 VGLUT<br />
2 VGLUT mRNA <br />
in situ hybridization <br />
<br />
<br />
2 VGLUT <br />
P<br />
C <br />
<br />
<br />
N 2 <br />
C PapillonLefevre <br />
HaimMunk <br />
C <br />
<br />
D <br />
<br />
8 <br />
<br />
CA2fasciola cinereumindusium griseumtenia tecta<br />
<br />
<br />
<br />
<br />
25<br />
8
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