第117åæ¥æ¬è§£åå¦ä¼ç·ä¼ã»å ¨å½å¦è¡éä¼ è¬æ¼ããã°ã©ã ã»æé²é PDF ...
第117åæ¥æ¬è§£åå¦ä¼ç·ä¼ã»å ¨å½å¦è¡éä¼ è¬æ¼ããã°ã©ã ã»æé²é PDF ...
第117åæ¥æ¬è§£åå¦ä¼ç·ä¼ã»å ¨å½å¦è¡éä¼ è¬æ¼ããã°ã©ã ã»æé²é PDF ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
174<br />
117 <br />
P<br />
Minocycline<br />
<br />
1 1,2 1 1,2 1 1<br />
1<br />
2 <br />
P<br />
ITAM KO <br />
1 1 2 1 1 1 <br />
2 1<br />
1<br />
2 <br />
<br />
<br />
<br />
<br />
<br />
SD 2 <br />
<br />
Minocycline 100 μg /day PBS 10 μl /day 8 <br />
1 8 Minocycline PBS <br />
von Frey<br />
test 2 1 <br />
1 <br />
OX42<br />
Minocycline PBS <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
ITAM DAP12 CARD9 KO <br />
L4 1 <br />
<br />
Iba1 GFAP <br />
KO <br />
Iba1 <br />
KO <br />
Iba1 <br />
GFAP <br />
KO GFAP <br />
DAP12 CARD9 ITAM <br />
<br />
<br />
<br />
<br />
P<br />
Zitter <br />
<br />
<br />
<br />
<br />
Zitter <br />
Zi<br />
Zi <br />
<br />
Iba1 Ca2+ <br />
ED1<br />
3 <br />
1Iba1 <br />
ED1 <br />
2<br />
ED1 3<br />
1 2 Iba1 <br />
ED1 1 3<br />
2 <br />
<br />
qPCR <br />
<br />
P<br />
<br />
1 1 2 1,3 1<br />
1<br />
2 <br />
3 <br />
<br />
<br />
<br />
<br />
DRG<br />
ERα <br />
GPR30 <br />
<br />
OVX <br />
OVX+E 4 <br />
<br />
OVX+E <br />
OVX <br />
<br />
<br />
DRG <br />
CGRP <br />
OVX+E OVX CGRP <br />
<br />
P<br />
Scaffold attachment factor B SAFB and SAFB synergistically<br />
inhibit intranuclear mobility and function of ERα<br />
<br />
<br />
Estrogen receptor alpha ERα plays a key role in physiological processes as a<br />
transcriptional factor that is regulated by cofactors. ERαmediated transcriptional<br />
regulation is correlated with the mobility of ERα in the nucleus associating with<br />
the nuclear matrix, the framework for nuclear events including transcription.<br />
However, the relationship between ERα mobility and the cofactors is unclear.<br />
Scaffold attachment factor B1 SAFB1 and its paralog SAFB2 are nuclear matrix<br />
binding proteins that have been characterized as ERα corepressors. Here, using<br />
chimeric fluorescent proteins, we show that SAFB1 and SAFB2 colocalize and<br />
interact with ERα in the nucleus of living cells after 17βestradiol E2 treatment.<br />
Fluorescence recovery after photobleaching analysis revealed that SAFB1 and<br />
SAFB2 each decrease ERα mobility, and coexpression of SAFB1 and SAFB2<br />
causes a synergistic reduction in ERα dynamics under E2 treatment. In accordance<br />
with these changes, ERαmediated transcription and proliferation is cooperatively<br />
inhibited by SAFB1 and SAFB2. These results indicate that SAFB1 and SAFB2<br />
are crucial repressors for ERα dynamics and function in association with the<br />
nuclear matrix.<br />
P<br />
Estrogen αfetoprotein <br />
<br />
<br />
Estrogen Aromatase <br />
Estrogen 20 <br />
Estrogen <br />
Estrogen 20 <br />
Estrogen <br />
αfetoprotein AFP Estrogen<br />
AFP Estrogen <br />
5-40 <br />
Wistar AFP Western<br />
blotting Realtime PCR <br />
Estradiol17β EIA kit 1 AFP<br />
5 20 2 <br />
AFP 20 3 AFP <br />
20 4 <br />
Aromatase AFP 5 AFP<br />
Estrogen AFP <br />
Aromatase <br />
AFP Estrogen
Change language