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P<br />
Characterization of the RNAbinding protein Musashi in zebrafish<br />
<br />
<br />
Musashi Msi is an evolutionarily conserved gene family of RNAbinding<br />
proteins RBPs that is preferentially expressed in the nervous system. The first<br />
member of the Msi family was identified in Drosophila. Drosophila Msi plays<br />
an important role in regulating asymmetric cell division of the sensory organ<br />
precursor cells. The mammalian orthologs, including human and mouse Msi1, are<br />
neural RBPs that are strongly expressed in neural stem cells NSCs. Mammalian<br />
Msi1 contributes to self renewal of NSCs through translational regulation of<br />
several target mRNAs. In this study, the zebrafish Msi ortholog zMsi1 was<br />
identified and characterized. The normal spatial and temporal expression profiles<br />
for both protein and mRNA were determined. Overall, zMsi1 was strongly<br />
expressed in neural tissue in early stages of development and exhibited similarity<br />
to mammalian Msi1 expression patterns. To reveal the in vivo function of zMsi1,<br />
morpholinos against Msi1 were introduced into onecell stage zebrafish embryos.<br />
Knock down of zmsi1 frequently resulted in aberrant formation of the CNS. These<br />
results suggest that Msi1 plays roles in CNS development in vertebrates.<br />
P<br />
PSANCAM <br />
1 2 1<br />
1<br />
2 <br />
<br />
PSANCAMpolysialic acidneural cell adhesion molecule <br />
<br />
PSANCAM 57 E5E7 <br />
PSANCAM <br />
<br />
PSANCAM <br />
BrdU E5E7 BrdU <br />
FITC <br />
PSANCAM <br />
BrdU 10 E6 <br />
BrdU <br />
E5 BrdU E6 E7 <br />
BrdU PSANCAM <br />
E5E7 PSANCAM <br />
<br />
<br />
P<br />
mRNA <br />
1 2 1<br />
1<br />
2 <br />
<br />
<br />
GnRH <br />
mRNA <br />
mRNA 2.5 3.5 <br />
3.5 <br />
4 GnRH <br />
mRNA <br />
mRNA <br />
<br />
Tol2GFP 2.5 <br />
1619 GFP <br />
GnRH GFP <br />
mRNA <br />
mRNA <br />
mRNA GnRH <br />
GnRH <br />
<br />
P<br />
CNTF <br />
<br />
<br />
<br />
<br />
<br />
cuprizone <br />
MBPCNPase <br />
<br />
<br />
<br />
<br />
cuprizone<br />
<br />
<br />
MBPCNPase <br />
<br />
CNTF <br />
<br />
CNTF <br />
P<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
E1313.5 E11.5<br />
<br />
Islet1Lim3Lhx3 MNR2 <br />
MMCl Lim3 <br />
<br />
Islet1Lim3Foxp1<br />
E11.5 <br />
Islet1 Lim3 <br />
<br />
Foxp1 <br />
<br />
P<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
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6