第117å›žæ—¥æœ¬è§£å‰–å­¦ä¼šç·ä¼šãƒ»å ¨å›½å­¦è¡“é›†ä¼š 講演プログラム・抄録集 PDF ...

117 157
P
αMangostin
1 2 3 1
1
2
3
HER2ER, PgR
αMangostin
MDAMB231 αMangostin
Caspase
c Realtime PCR
Caspase389
Caspase4
c G1 S
p21 Cip1
PCNACyclin D1cdc25ACdK2 Cdk6
Aktp38α ERK1/2
αMangostin Akt
p21 Cip1 G1 G1
P
Sevoflurane rat Per
1,2 1 2 1
1
2
Per2 suprachiasmatic
nucleus: SCN 24
mouse Per2 sevoflurane
Ohe et al, 2010 SCN Per2
sevoflurane SCN
Wister rat Per2 rPer2 mRNA
RI in situ hybridizasion rPer2
Per2
rPer2
SCN rPer2
25 rPer2
sevofrurane
SCN
Per2
P
Transverse anchoring system of myofibril to sarcolemma: the
morphological study
Khairani Astrid Feinisa, Yuki Tajika, Maiko Takahashi, Hitoshi Ueno,
Tohru Murakami, Hiroshi Yorifuji
Grad.Sch.of Medicine Gunma Univ.
Transverse anchoring system of myofibril to sarcolemma is subplasmalemmal
cytoskeleton network that is anchored into the sarcolemma and provides an
overall diffuse protection system of skeletal muscle. Three types of filament have
been indicated as the transverse anchoring system; γ actin, desmin, and keratin.
We have examined these subsarcolemmal cytoskeletal components in diaphragm
and lower limb muscles of adult wild type and mdx mice by electron microscopy
EM and immunohistochemistry. Isolated single fibers as well as small blocks
of the muscles are used for both methods. EM revealed that the intermediate
filaments seemed to either connect between the sarcolemma and the Zdisc and
Mline or run as a part of subsarcolemmal density. These filamentous networks
apparently decrease in mdx mice. These data suggest a morphological model of
transverse anchoring system and how it affects the functions of skeletal muscle.
P
Khairani Astrid Feinisa
SNARE
2 SNARE
SNARE
P
Prosaposin expression in cardiac muscle of mdx mice in early period
of disease
1 1 1 1 1 2
1
1
2
This study investigated the expression of the trophic factor prosaposin in the
cardiac muscles of mdx mice. Prosaposin is strongly expressed in adult rat
cardiac and skeletal muscles and plays a myotrophic role in vitro. However, few
studies have focused on the role of prosaposin during muscle regeneration in mdx
mice, which do not express the dystrophin gene. Dystrophin deficiency leads to
progressive cardiac and skeletal muscle pathology. We examined the expression
of prosaposin in the cardiac muscle in mdx and C57BL mice using HE staining,
immunohistochemistry, and in situ hybridization in 4weekold mice. HE staining
showed no obvious change in morphology of mdx cardiac muscles compared
with control mice. The results of in situ hybridization showed that the main form
of prosaposin mRNA is PS+0 in the cardiac muscle of mdx mice, and the mRNA
expression is obviously lower in the cardiac muscles of mdx mice. Prosaposin
protein also decreased in mdx cardiac muscle. These results suggest that the
change of prosaposin expression happened earlier than morphological change in
cardiac muscle of mdx mice.
P
14
30%ST
96 21
34 56 58 42